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Literature summary extracted from

  • Ugochukwu, E.; Lovering, A.L.; Mather, O.C.; Young, T.W.; White, S.A.
    The crystal structure of the cytosolic exopolyphosphatase from Saccharomyces cerevisiae reveals the basis for substrate specificity (2007), J. Mol. Biol., 371, 1007-1021.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.6.1.11 gene PPX1, overexpression Saccharomyces cerevisiae

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.6.1.11 enzxme in complex with phosphate, sulfate, or ATP, X-ray diffraction structure determination and analysis at 1.6, 1.8, and 1.9 A resolution, multiple isomorphous replacement with anomalous scattering technique Saccharomyces cerevisiae

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.6.1.11 cytosol
-
 Saccharomyces cerevisiae 5829
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.6.1.11 45058
-
 x * 45058, recombinant enzyme, mass spectrometry Saccharomyces cerevisiae

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.6.1.11 (phosphate)n + H2O Saccharomyces cerevisiae
-
 (phosphate)n-1 + phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.6.1.11 Saccharomyces cerevisiae
-
 gene PPX1
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.6.1.11 recombinant PPX1 17.4fold in a two-step procedure Saccharomyces cerevisiae

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.6.1.11 2070
-
 purified recombinant enzyme Saccharomyces cerevisiae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.6.1.11 (phosphate)n + H2O
-
 Saccharomyces cerevisiae (phosphate)n-1 + phosphate
-
 ?
3.6.1.11 (phosphate)n + H2O the cytosolic exopolyphosphatase that processively cleaves the terminal phosphate group from the polyphosphate chain, until inorganic diphosphate is all that remains, structure of the substrate binding channel, overview Saccharomyces cerevisiae (phosphate)n-1 + phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
3.6.1.11 ? x * 45058, recombinant enzyme, mass spectrometry Saccharomyces cerevisiae

Synonyms

EC Number Synonyms Comment Organism
3.6.1.11 More PPX1 belongs to the DHH family of phosphoesterases Saccharomyces cerevisiae
3.6.1.11 PPX1
-
 Saccharomyces cerevisiae

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.6.1.11 1550
-
 (Phosphate)n 37°C, recombinant enzyme Saccharomyces cerevisiae