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Literature summary extracted from

  • Petit, P.; Granier, T.; dEstaintot, B.L.; Manigand, C.; Bathany, K.; Schmitter, J.M.; Lauvergeat, V.; Hamdi, S.; Gallois, B.
    Crystal structure of grape dihydroflavonol 4-reductase, a key enzyme in flavonoid biosynthesis (2007), J. Mol. Biol., 368, 1345-1357.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.219 expression in Escherichia coli Vitis vinifera

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.1.1.219 purified recombinant enzyme in complex with NADPH and dihydroquercetin, X-ray diffraction structure determination and analysis at 1.8 A resolution Vitis vinifera

Protein Variants

EC Number Protein Variants Comment Organism
1.1.1.219 N133D site-directed mutagenesis, Vitis vinifera

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.219 dihydrokaempferol + NADPH Vitis vinifera
-
 cis-3,4-leucopelargonidin + NADP+
-
 r
1.1.1.219 dihydromyricetin + NADPH Vitis vinifera
-
 leucodelphinidin + NADP+
-
 r
1.1.1.219 dihydroquercetin + NADPH Vitis vinifera
-
 leucocyanidin + NADP+
-
 r
1.1.1.219 additional information Vitis vinifera the key enzyme in flavonoid biosynthesis catalyzes a late step in the biosynthesis of anthocyanins and condensed tannins, two flavonoid classes of importance to plant survival and human nutrition, overview ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.219 Vitis vinifera
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.219 recombinanet enzyme from Escherichia coli Vitis vinifera

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.219 dihydrokaempferol + NADPH
-
 Vitis vinifera cis-3,4-leucopelargonidin + NADP+
-
 r
1.1.1.219 dihydromyricetin + NADPH
-
 Vitis vinifera leucodelphinidin + NADP+
-
 r
1.1.1.219 dihydroquercetin + NADPH
-
 Vitis vinifera leucocyanidin + NADP+
-
 r
1.1.1.219 additional information the key enzyme in flavonoid biosynthesis catalyzes a late step in the biosynthesis of anthocyanins and condensed tannins, two flavonoid classes of importance to plant survival and human nutrition, overview Vitis vinifera ?
-
 ?
1.1.1.219 additional information the specific residue at position 133, Asn or Asp, is involved in controling of the substrate recognition and recognition of the B-ring hydroxylation pattern of dihydroflavonols, structure-function relationship, overview Vitis vinifera ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.1.219 More 3D structure of the ternary complex, overview Vitis vinifera

Synonyms

EC Number Synonyms Comment Organism
1.1.1.219 DFR
-
 Vitis vinifera
1.1.1.219 dihydroflavonol 4-reductase
-
 Vitis vinifera

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.219 NADP+ binding site structure, overview Vitis vinifera
1.1.1.219 NADPH
-
 Vitis vinifera