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Literature summary extracted from

  • Bong, S.M.; Moon, J.H.; Jang, E.H.; Lee, K.S.; Chi, Y.M.
    Overexpression, purification, and preliminary X-ray crystallographic analysis of human brain-type creatine kinase (2008), J. Microbiol. Biotechnol., 18, 295-298.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.3.2 overexpressed as a soluble form in Escherichia coli Homo sapiens

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.7.3.2 crystallized at 22°C using PEG 4000 as a precipitant. X-ray diffraction data are collected to 2.2 A resolution using synchrotron radiation. The crystals belong to the tetragonal space group P4(3)2(1)2, with cell parameters of a = b = 97.963, c = 164.312 A, and alpha = beta = gamma = 90°. The symmetric unit contains two molecules of creatine kinase Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
2.7.3.2 Homo sapiens
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.3.2
-
 Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.3.2 brain
-
 Homo sapiens
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.3.2 ATP + creatine the brain-type cytosolic isoform of creatine kinase, which is found mainly in the brain and retina, is a key enzyme in brain energy metabolism, because high-energy phosphates are transfered through the creatine kinase/phosphocreatine shuttle system Homo sapiens ADP + phosphocreatine
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.7.3.2 BB-CK brain-type cytosolic isoform of creatine kinase Homo sapiens