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Literature summary extracted from

  • Das, A.; Davis, M.A.; Rudel, L.L.
    Identification of putative active site residues of ACAT enzymes (2008), J. Lipid Res., 49, 1770-1781.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.1.26 into the PCDNA3 vector Chlorocebus aethiops
2.3.1.26 into the PCDNA3 vector Chlorocebus sabaeus

Protein Variants

EC Number Protein Variants Comment Organism
2.3.1.26 A1D400N mutant, evaluation of conserved aspartic acid residues Chlorocebus aethiops
2.3.1.26 A1D406A mutant, evaluation of conserved aspartic acid residues Chlorocebus aethiops
2.3.1.26 A1H386N mutant, identification of essential histidines required for activity Chlorocebus aethiops
2.3.1.26 A1H425A mutant, identification of essential histidines required for activity Chlorocebus aethiops
2.3.1.26 A1H460A mutant, identification of essential histidines required for activity Chlorocebus aethiops
2.3.1.26 A1S128A mutant, identification of the role of conserved serine residues in determination of the activity Chlorocebus aethiops
2.3.1.26 A1S194A mutant, identification of the role of conserved serine residues in determination of the activity Chlorocebus aethiops
2.3.1.26 A1S269A mutant, identification of the role of conserved serine residues in determination of the activity Chlorocebus aethiops
2.3.1.26 A1S410A mutant, identification of the role of conserved serine residues in determination of the activity Chlorocebus aethiops
2.3.1.26 A1S412A mutant, identification of the role of conserved serine residues in determination of the activity Chlorocebus aethiops
2.3.1.26 A1S414L mutant, identification of the role of conserved serine residues in determination of the activity Chlorocebus aethiops
2.3.1.26 A1S456A mutant, identification of the role of conserved serine residues in determination of the activity Chlorocebus aethiops
2.3.1.26 A1Y142F mutant, evaluation of the role of conserved tyrosine residues as a requirement for the activity Chlorocebus aethiops
2.3.1.26 A1Y308F mutant, evaluation of the role of conserved tyrosine residues as a requirement for the activity Chlorocebus aethiops
2.3.1.26 A1Y312F mutant, evaluation of the role of conserved tyrosine residues as a requirement for the activity Chlorocebus aethiops
2.3.1.26 A1Y322F mutant, evaluation of the role of conserved tyrosine residues as a requirement for the activity Chlorocebus aethiops
2.3.1.26 A1Y404F mutant, evaluation of the role of conserved tyrosine residues as a requirement for the activity Chlorocebus aethiops
2.3.1.26 A1Y433F mutant, evaluation of the role of conserved tyrosine residues as a requirement for the activity Chlorocebus aethiops
2.3.1.26 A1Y518F mutant, evaluation of the role of conserved tyrosine residues as a requirement for the activity Chlorocebus aethiops
2.3.1.26 A2D378E mutant, evaluation of conserved aspartic acid residues Chlorocebus sabaeus
2.3.1.26 A2D384A mutant, evaluation of conserved aspartic acid residues Chlorocebus sabaeus
2.3.1.26 A2H364N mutant, identification of essential histidines required for activity Chlorocebus sabaeus
2.3.1.26 A2H403A mutant, identification of essential histidines required for activity Chlorocebus sabaeus
2.3.1.26 A2H438A mutant, identification of essential histidines required for activity Chlorocebus sabaeus
2.3.1.26 A2S109A mutant, identification of the role of conserved serine residues in determination of the activity Chlorocebus sabaeus
2.3.1.26 A2S176A mutant, identification of the role of conserved serine residues in determination of the activity Chlorocebus sabaeus
2.3.1.26 A2S249A mutant, identification of the role of conserved serine residues in determination of the activity Chlorocebus sabaeus
2.3.1.26 A2S388A mutant, identification of the role of conserved serine residues in determination of the activity Chlorocebus sabaeus
2.3.1.26 A2S390A mutant, identification of the role of conserved serine residues in determination of the activity Chlorocebus sabaeus
2.3.1.26 A2S392A mutant, identification of the role of conserved serine residues in determination of the activity Chlorocebus sabaeus
2.3.1.26 A2S434A mutant, identification of the role of conserved serine residues in determination of the activity Chlorocebus sabaeus
2.3.1.26 A2Y124F mutant, evaluation of the role of conserved tyrosine residues as a requirement for the activity Chlorocebus sabaeus
2.3.1.26 A2Y286F mutant, evaluation of the role of conserved tyrosine residues as a requirement for the activity Chlorocebus sabaeus
2.3.1.26 A2Y290F mutant, evaluation of the role of conserved tyrosine residues as a requirement for the activity Chlorocebus sabaeus
2.3.1.26 A2Y300F mutant, evaluation of the role of conserved tyrosine residues as a requirement for the activity Chlorocebus sabaeus
2.3.1.26 A2Y382F mutant, evaluation of the role of conserved tyrosine residues as a requirement for the activity Chlorocebus sabaeus
2.3.1.26 A2Y411F mutant, evaluation of the role of conserved tyrosine residues as a requirement for the activity Chlorocebus sabaeus
2.3.1.26 A2Y496F mutant, evaluation of the role of conserved tyrosine residues as a requirement for the activity Chlorocebus sabaeus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.26 acyl-CoA + cholesterol Chlorocebus aethiops
-
 CoA + cholesterol ester
-
 ?
2.3.1.26 acyl-CoA + cholesterol Chlorocebus sabaeus
-
 CoA + cholesterol ester
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.26 Chlorocebus aethiops
-
 African green monkey
-
2.3.1.26 Chlorocebus sabaeus
-
 African green monkey
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.1.26 microsomes from AC29 cells are prepared Chlorocebus aethiops
2.3.1.26 microsomes from AC29 cells are prepared Chlorocebus sabaeus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.26 acyl-CoA + cholesterol
-
 Chlorocebus aethiops CoA + cholesterol ester
-
 ?
2.3.1.26 acyl-CoA + cholesterol
-
 Chlorocebus sabaeus CoA + cholesterol ester
-
 ?
2.3.1.26 oleoyl-CoA + cholesterol activity assay Chlorocebus sabaeus CoA + cholesteryl oleate
-
 ?
2.3.1.26 oleoyl-CoA + cholesterol activity assay Chlorocebus aethiops ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.3.1.26 ACAT1
-
 Chlorocebus aethiops
2.3.1.26 ACAT2
-
 Chlorocebus sabaeus
2.3.1.26 acyl-CoA:cholesterol acyltransferase 1
-
 Chlorocebus aethiops
2.3.1.26 acyl-CoA:cholesterol acyltransferase 2
-
 Chlorocebus sabaeus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.3.1.26 37
-
 microsomal ACAT assay Chlorocebus aethiops
2.3.1.26 37
-
 microsomal ACAT assay Chlorocebus sabaeus