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Literature summary extracted from
- Widespread occurrence of glyceryl ether monooxygenase activity in rat tissues detected by a novel assay (2007), J. Lipid Res., 48, 1422-1427.
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.14.16.5 | 0.0026 | - |
(6R)-tetrahydrobiopterin | pH 8.5, 37°C | Rattus norvegicus |  |
| 1.14.16.5 | 0.0089 | - |
1-O-pyrenedecyl-sn-glycerol | pH 8.5, 37°C | Rattus norvegicus | |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 1.14.16.5 | microsome | - |
Rattus norvegicus | - |
- |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.14.16.5 | 1-alkyl-sn-glycerol + tetrahydrobiopterin + O2 | Rattus norvegicus | - |
1-hydroxyalkyl-sn-glycerol + dihydrobiopterin + H2O | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.14.16.5 | Rattus norvegicus | - |
Sprague-Dawley rats | - |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 1.14.16.5 | partial, 100fold purification of the native enzyme from liver microsomes | Rattus norvegicus |
Reaction
| EC Number | Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|---|
| 1.14.16.5 | 1-O-alkyl-sn-glycerol + a 5,6,7,8-tetrahydropteridine + O2 = 1-O-(1-hydroxyalkyl)-sn-glycerol + a 4a-hydroxy-5,6,7,8-tetrahydropteridine | The glyceryl ether substrate is first hydroxylated in a pteridine-dependent monooxygenase reaction to yield the unstable semiacetal product. The enzyme accepts a range of chain lengths from n = 11 to n = 20. The tetrahydrobiopterin cofactor is first converted to a 4alpha-hydroxy derivative and leaves the reaction after dehydration as 6,7[8H]-dihydrobiopterin, which is recycled to tetrahydrobiopterin by dihydropteridine reductase with NADH or NADPH. The semiacetal rearranges to the corresponding aldehyde and glycerol. In the presence of oxygen, the aldehyde is readily oxidized to the corresponding acid | Rattus norvegicus |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 1.14.16.5 | brain | - |
Rattus norvegicus | - |
| 1.14.16.5 | cerebellum | - |
Rattus norvegicus | - |
| 1.14.16.5 | cerebral cortex | - |
Rattus norvegicus | - |
| 1.14.16.5 | heart | - |
Rattus norvegicus | - |
| 1.14.16.5 | kidney | - |
Rattus norvegicus | - |
| 1.14.16.5 | large intestine | - |
Rattus norvegicus | - |
| 1.14.16.5 | liver | - |
Rattus norvegicus | - |
| 1.14.16.5 | lung | - |
Rattus norvegicus | - |
| 1.14.16.5 | additional information | wide tissue distribution, except female heart, with highest activities in liver, intestine, and cerebellum, overview | Rattus norvegicus | - |
| 1.14.16.5 | ovary | - |
Rattus norvegicus | - |
| 1.14.16.5 | skeletal muscle | - |
Rattus norvegicus | - |
| 1.14.16.5 | small intestine | - |
Rattus norvegicus | - |
| 1.14.16.5 | spleen | - |
Rattus norvegicus | - |
| 1.14.16.5 | stomach | - |
Rattus norvegicus | - |
| 1.14.16.5 | striatum | - |
Rattus norvegicus | - |
| 1.14.16.5 | testis | - |
Rattus norvegicus | - |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.14.16.5 | 1-alkyl-sn-glycerol + tetrahydrobiopterin + O2 | - |
Rattus norvegicus | 1-hydroxyalkyl-sn-glycerol + dihydrobiopterin + H2O | - |
? | |
| 1.14.16.5 | 1-O-pyrenedecyl-sn-glycerol + tetrahydrobiopterin + O2 | 1-O-pyrenedecyl-sn-glycerol and high-performance liquid chromatographic analysis of reaction mixtures with fluorescence detection, overview | Rattus norvegicus | 1-hydroxypyrenedecyl-sn-glycerol + dihydrobiopterin + H2O | further formation of 1-pyrenedecanoic acid | ? | |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 1.14.16.5 | glyceryl ether monooxygenase | - |
Rattus norvegicus |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.14.16.5 | 37 | - |
assay at | Rattus norvegicus |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.14.16.5 | 8.5 | - |
assay at | Rattus norvegicus |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.14.16.5 | (6R)-tetrahydrobiopterin | strictly dependent on, stereospecific, overview | Rattus norvegicus | |
| 1.14.16.5 | (6RS)-6,7-dimethyltetrahydropterin | 94.2% of the activity with 6R-tetrahydrobiopterin | Rattus norvegicus | |
| 1.14.16.5 | (6RS)-6-hydroxymethyltetrahydropterin | 16% of the activity with 6R-tetrahydrobiopterin | Rattus norvegicus | |
| 1.14.16.5 | (6RS)-6-methyltetrahydropterin | 60.9% of the activity with 6R-tetrahydrobiopterin | Rattus norvegicus | |
| 1.14.16.5 | (6RS)-tetrahydrodictyopterin | 44.7% of the activity with 6R-tetrahydrobiopterin | Rattus norvegicus | |
| 1.14.16.5 | (6RS)-tetrahydroneopterin | 124% of the activity with 6R-tetrahydrobiopterin | Rattus norvegicus | |
| 1.14.16.5 | (6S)-tetrahydrobiopterin | 3.5% of the activity with 6R-tetrahydrobiopterin | Rattus norvegicus | |
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