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Literature summary extracted from

  • Pirillo, A.; Uboldi, P.; Bolego, C.; Kuhn, H.; Catapano, A.L.
    The 15-lipoxygenase-modified high density lipoproteins 3 fail to inhibit the TNF-alpha-induced inflammatory response in human endothelial cells (2008), J. Immunol., 181, 2821-2830.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.13.11.33 overexpression of His-tagged enzyme in Escherichia coli Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.13.11.33 arachidonate + O2 Homo sapiens the enzyme modifies high density lipoprotein 3, the major and most antiatherogenic HDL subfraction, and impairs anti-inflammatory activity of the lipoprotein, which, after modification, fails to inhibit TNFalpha-mediated mRNA and protein induction of adhesion molecules and monocyte chemoattractant protein-1, MCP-1, in endothelial cells, overview (5Z,8Z,11Z,13E)-(15S)-15-hydroperoxyeicosa-5,8,11,13-tetraenoate is metabolized to 15(S)-hydroxyeicosatetranoic, i.e. 15(S)-HETE ?

Organism

EC Number Organism UniProt Comment Textmining
1.13.11.33 Homo sapiens
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.13.11.33 recombinant His-tagged enzyme from Escherichia coli coli by nickel affinity and anion exchange chromatography Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.13.11.33 additional information the enzyme is overexpressed in atherosclerotic lesions Homo sapiens
-
1.13.11.33 umbilical vein endothelial cell HUVEC Homo sapiens
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.13.11.33 arachidonate + O2
-
 Homo sapiens (5Z,8Z,11Z,13E)-(15S)-15-hydroperoxyeicosa-5,8,11,13-tetraenoate
-
 ?
1.13.11.33 arachidonate + O2 the enzyme modifies high density lipoprotein 3, the major and most antiatherogenic HDL subfraction, and impairs anti-inflammatory activity of the lipoprotein, which, after modification, fails to inhibit TNFalpha-mediated mRNA and protein induction of adhesion molecules and monocyte chemoattractant protein-1, MCP-1, in endothelial cells, overview Homo sapiens (5Z,8Z,11Z,13E)-(15S)-15-hydroperoxyeicosa-5,8,11,13-tetraenoate is metabolized to 15(S)-hydroxyeicosatetranoic, i.e. 15(S)-HETE ?
1.13.11.33 linoleic acid + O2
-
 Homo sapiens ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.13.11.33 15-lipoxygenase
-
 Homo sapiens

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.13.11.33 4
-
 linoleic acid recombinant enzyme Homo sapiens