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Literature summary extracted from

  • Isobe, K.; Kato, A.; Ogawa, J.; Kataoka, M.; Iwasaki, A.; Hasegawa, J.; Shimizu, S.
    Characterization of alcohol oxidase from Aspergillus ochraceus AIU 031 (2007), J. Gen. Appl. Microbiol., 53, 177-183.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.3.13 hydrazine 1 mM, complete inhibition Aspergillus ochraceus
1.1.3.13 hydroxylamine 1 mM, complete inhibition Aspergillus ochraceus
1.1.3.13 Monoiodoacetic acid 1 mM, 31% inhibition Aspergillus ochraceus
1.1.3.13 phenylhydrazine 1 mM, complete inhibition Aspergillus ochraceus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.3.13 1.25
-
 ethylene glycol
-
 Aspergillus ochraceus
1.1.3.13 12
-
 ethanol
-
 Aspergillus ochraceus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.3.13 68000
-
 4 * 68000, SDS-PAGE Aspergillus ochraceus
1.1.3.13 262000
-
 gel filtration Aspergillus ochraceus

Organism

EC Number Organism UniProt Comment Textmining
1.1.3.13 Aspergillus ochraceus
-
 AIU 031
-
1.1.3.13 Aspergillus ochraceus AIU 031
-
 AIU 031
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.3.13
-
 Aspergillus ochraceus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.3.13 culture condition when incubated in the cholesterol medium, the alcohol oxidase activity reaches the maximum at 2 days of cultivation, and then gradually decreases. Methanol, ethanol and isopropanol are also effective for production of alcohol oxidase, but glycerol, glucose and malic acid are not Aspergillus ochraceus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.3.13 3.59
-
 oxidation of ethanol Aspergillus ochraceus
1.1.3.13 3.64
-
 oxidation of ethylene glycol Aspergillus ochraceus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.3.13 1-butanol + O2 49% of the activity with methanol Aspergillus ochraceus n-butanal + H2O2
-
 ?
1.1.3.13 1-butanol + O2 49% of the activity with methanol Aspergillus ochraceus AIU 031 n-butanal + H2O2
-
 ?
1.1.3.13 1-propanol + O2 66% of the activity with methanol Aspergillus ochraceus n-propanal + H2O2
-
 ?
1.1.3.13 1-propanol + O2 66% of the activity with methanol Aspergillus ochraceus AIU 031 n-propanal + H2O2
-
 ?
1.1.3.13 ethanol + O2 97% of the activity with methanol Aspergillus ochraceus acetaldehyde + H2O2
-
 ?
1.1.3.13 ethanol + O2 97% of the activity with methanol Aspergillus ochraceus AIU 031 acetaldehyde + H2O2
-
 ?
1.1.3.13 ethylene glycol + O2 12% of the activity with methanol Aspergillus ochraceus ?
-
 ?
1.1.3.13 ethylene glycol + O2 12% of the activity with methanol Aspergillus ochraceus AIU 031 ?
-
 ?
1.1.3.13 methanol + O2
-
 Aspergillus ochraceus formaldehyde + H2O2
-
 ?
1.1.3.13 methanol + O2
-
 Aspergillus ochraceus AIU 031 formaldehyde + H2O2
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.3.13 tetramer 4 * 68000, SDS-PAGE Aspergillus ochraceus

Synonyms

EC Number Synonyms Comment Organism
1.1.3.13 AOd
-
 Aspergillus ochraceus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.3.13 50 55 ethanol oxidation Aspergillus ochraceus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.1.3.13 40
-
 0.1 M potassium phosphate buffer, pH 7.0, 20 min, almost all the enzyme activity remains Aspergillus ochraceus
1.1.3.13 50
-
 0.1 M potassium phosphate buffer, pH 7.0, 20 min, about 60% of the activity remains Aspergillus ochraceus
1.1.3.13 55
-
 0.1 M potassium phosphate buffer, pH 7.0, 20 min, about 25% of the activity remains Aspergillus ochraceus
1.1.3.13 60
-
 0.1 M potassium phosphate buffer, pH 7.0, 20 min, complete inactivation Aspergillus ochraceus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.3.13 5.5 7 ethanol oxidation Aspergillus ochraceus

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.1.3.13 6
-
 40°C, 20 min, about 60% loss of activity Aspergillus ochraceus
1.1.3.13 6.5 8.5 40°C, 20 min, more than 90% of the activity remains Aspergillus ochraceus

pI Value

EC Number Organism Comment pI Value Maximum pI Value
1.1.3.13 Aspergillus ochraceus isoelectric focusing, pH-range: 3.5-10
-
 5.4