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Literature summary extracted from

  • Lagana, G.; Giacobbe, S.; Bellocco, E.; Mannucci, C.; Galtieri, A.; Ficarra, S.; Kotyk, A.; Leuzzi, U.
    Lactate and malate dehydrogenase in the fan-shell associated shrimp, Pontonia pinnophylax (Otto): effects of temperature and urea (2007), J. Exp. Mar. Biol. Ecol., 349, 27-34.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.27 Urea enzyme activity and electrophoretic pattern of LDH-A4 and malate dehydrogenase, EC 1.1.1.37, compared in relation to heat and urea inactivation, LDH is more sensitive than MDH, overview Pontonia pinnophylax
1.1.1.37 Urea enzyme activity and electrophoretic pattern of MDH and lactate dehydrogenase, EC 1.1.1.27, compared in relation to heat and urea inactivation, LDH is more sensitive than MDH, overview Pontonia pinnophylax

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.1.1.27 cytosol
-
 Pontonia pinnophylax 5829
-
1.1.1.37 cytosol cytosolic isozyme Pontonia pinnophylax 5829
-
1.1.1.37 mitochondrion mitochondrial isozyme Pontonia pinnophylax 5739
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.27 (S)-lactate + NAD+ Pontonia pinnophylax
-
 pyruvate + NADH + H+
-
 r
1.1.1.37 (S)-malate + NAD+ Pontonia pinnophylax
-
 oxaloacetate + NADH + H+
-
 r

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.27 Pontonia pinnophylax
-
 inhabiting the mantle cavity of host Pinna nobilis
-
1.1.1.37 Pontonia pinnophylax
-
 inhabiting the mantle cavity of host Pinna nobilis
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.37 native enzyme partially by subcellular fractionation Pontonia pinnophylax

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.27 additional information
-
 enzyme activity and electrophoretic pattern of LDH-A4 and malate dehydrogenase, EC 1.1.1.37, compared in relation to heat and urea inactivation, overview Pontonia pinnophylax
1.1.1.37 additional information
-
 enzyme activity and electrophoretic pattern of MDH and lactate dehydrogenase, EC 1.1.1.27, compared in relation to heat and urea inactivation, overview Pontonia pinnophylax

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.27 (S)-lactate + NAD+
-
 Pontonia pinnophylax pyruvate + NADH + H+
-
 r
1.1.1.27 (S)-lactate + NAD+ enzyme activity and electrophoretic pattern of LDH-A4 and malate dehydrogenase, EC 1.1.1.37, compared in relation to heat and urea inactivation, overview Pontonia pinnophylax pyruvate + NADH + H+
-
 r
1.1.1.37 (S)-malate + NAD+
-
 Pontonia pinnophylax oxaloacetate + NADH + H+
-
 r
1.1.1.37 (S)-malate + NAD+ enzyme activity and electrophoretic pattern of MDH and lactate dehydrogenase, EC 1.1.1.27, compared in relation to heat and urea inactivation, overview Pontonia pinnophylax oxaloacetate + NADH + H+
-
 r

Subunits

EC Number Subunits Comment Organism
1.1.1.27 More enzyme activity and electrophoretic pattern of LDH-A4 and malate dehydrogenase, EC 1.1.1.37, compared in relation to heat and urea inactivation, overview Pontonia pinnophylax
1.1.1.37 More enzyme activity and electrophoretic pattern of MDH and lactate dehydrogenase, EC 1.1.1.27, compared in relation to heat and urea inactivation, overview Pontonia pinnophylax

Synonyms

EC Number Synonyms Comment Organism
1.1.1.27 lactate dehydrogenase
-
 Pontonia pinnophylax
1.1.1.27 LDH
-
 Pontonia pinnophylax
1.1.1.27 LDH-A4
-
 Pontonia pinnophylax
1.1.1.37 malate dehydrogenase
-
 Pontonia pinnophylax
1.1.1.37 MDH
-
 Pontonia pinnophylax

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.1.27 25
-
 assay at, pyruvate reduction Pontonia pinnophylax
1.1.1.37 25
-
 assay at, oxaloacetate reduction Pontonia pinnophylax

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.1.1.27 65
-
 enzyme activity and electrophoretic pattern of LDH-A4 and malate dehydrogenase, EC 1.1.1.37, compared in relation to heat and urea inactivation, MDH is more sensitive than LDH, overview Pontonia pinnophylax
1.1.1.37 65
-
 enzyme activity and electrophoretic pattern of MDH and lactate dehydrogenase, LDH EC 1.1.1.27, compared in relation to heat and urea inactivation, MDH is more sensitive than LDH, overview Pontonia pinnophylax

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.27 7.1
-
 assay at, pyruvate reduction Pontonia pinnophylax
1.1.1.37 7.5
-
 assay at, oxaloacetate reduction Pontonia pinnophylax

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.27 NAD+
-
 Pontonia pinnophylax
1.1.1.27 NADH
-
 Pontonia pinnophylax
1.1.1.37 NAD+
-
 Pontonia pinnophylax
1.1.1.37 NADH
-
 Pontonia pinnophylax