EC Number | Activating Compound | Comment | Organism | Structure |
---|---|---|---|---|
3.5.5.1 | indole-3-acetonitrile | induces protein expression | Zea mays | |
3.5.5.1 | additional information | a 2fold induction by light is observed | Zea mays | |
3.5.5.4 | 3-Cyano-L-alanine | induces protein expression | Zea mays | |
3.5.5.4 | additional information | a 2fold induction by light is observed | Zea mays |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
3.5.5.1 | jasmonic acid | application of jasmonic acid represses protein expression | Zea mays | |
3.5.5.1 | additional information | gibberellic acid, kinetin, abscisic acid, or epi-brassinolide have no obvious influence on activity | Zea mays | |
3.5.5.4 | jasmonic acid | application of jasmonic acid represses protein expression | Zea mays | |
3.5.5.4 | additional information | gibberellic acid, kinetin, abscisic acid, or epi-brassinolide have no obvious influence on activity | Zea mays |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.5.5.1 | 0.9 | - |
indole-3-acetonitrile | - |
Zea mays | |
3.5.5.4 | 1.8 | - |
3-Cyano-L-alanine | - |
Zea mays |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.5.5.1 | Zea mays | - |
line B73 | - |
3.5.5.4 | Zea mays | - |
line B73 | - |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.5.5.1 | Ni-NTA agarose column chromatography and HiLoad 16/60 Superdex 200 gel filtration | Zea mays |
3.5.5.4 | Ni-NTA agarose column chromatography and HiLoad 16/60 Superdex 200 gel filtration | Zea mays |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
3.5.5.1 | root | - |
Zea mays | - |
3.5.5.4 | root | - |
Zea mays | - |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.5.5.1 | indole-3-acetonitrile + H2O | preferred substrate of NIT2 | Zea mays | indole-3-acetic acid + NH3 | - |
? | |
3.5.5.1 | additional information | NIT2 does not use 3-cyano-L-alanine as a substrate, however, turnover of 3-cyano-L-alanine function is acquired when NIT2 forms heteromers with the homologue NIT1 | Zea mays | ? | - |
? | |
3.5.5.4 | 3-cyano-L-alanine + H2O | - |
Zea mays | L-aspartate + NH3 | - |
? | |
3.5.5.4 | additional information | does not use indole-3-acetonitrile as a substrate | Zea mays | ? | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.5.5.1 | NIT2 | - |
Zea mays |
3.5.5.4 | NIT1 | - |
Zea mays |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.5.5.1 | 0.13 | - |
indole-3-acetonitrile | - |
Zea mays | |
3.5.5.4 | 0.001 | - |
3-Cyano-L-alanine | - |
Zea mays |