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Literature summary extracted from

  • Sakiroglu, H.; Oztuerk, A.E.; Pepe, A.E.; Erat, M.
    Some kinetic properties of polyphenol oxidase obtained from dill (Anethum graveolens) (2008), J. Enzyme Inhib. Med. Chem., 23, 380-385.
    View publication on PubMed

Application

EC Number Application Comment Organism
1.14.18.1 nutrition the enzyme is a target for development of specific inhibitors to avoid unfavorable enzymatic browning of plant-derived foods. The inhibitors used in this study can be used safely in making herb cheese Anethum graveolens

General Stability

EC Number General Stability Organism
1.14.18.1 The stability of the enzyme is determined by measuring activity in the various buffers (0.1 M citrate/0.2 M phosphate for pH 3.5-5.0, 0.2 M phosphate for pH 5.5-7.5) every 3 days using catechol as substrate under optimum conditions (pH, temperature and ionic strength). Anethum graveolens

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.14.18.1 ascorbic acid noncompetitve Anethum graveolens
1.14.18.1 glutathione noncompetitve Anethum graveolens
1.14.18.1 L-cysteine most effective inhibitor, noncompetitve Anethum graveolens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.14.18.1 0.835
-
 chlorogenic acid PPO of dill has a great affinity towards chlorogenic acid. calculated from the Lineweaver-Burk graphs for substrates Anethum graveolens
1.14.18.1 1.67
-
 dopamine calculated from the Lineweaver-Burk graphs for substrates Anethum graveolens
1.14.18.1 2.17
-
 catechol calculated from the Lineweaver-Burk graphs for substrates Anethum graveolens

Organism

EC Number Organism UniProt Comment Textmining
1.14.18.1 Anethum graveolens
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.14.18.1 partially purified from dill by (NH4)2SO4 percipititation followed by dialysis and gel filtration chromatography Anethum graveolens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.14.18.1 leaf kept for 2 days in the refrigerator at 4°C before PPO extraction Anethum graveolens
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.14.18.1 additional information
-
 activity is measured spectrophotometrically at 420 nm, 13530.07 EU/mg of protein, gel filtration chromatography Anethum graveolens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.18.1 catechol + 1/2 O2
-
 Anethum graveolens 1,2-benzoquinone + H2O
-
 ?
1.14.18.1 chlorogenic acid + 1/2 O2 best substrate Anethum graveolens chlorogenoquinone + H2O
-
 ?
1.14.18.1 dopamine + 1/2 O2
-
 Anethum graveolens dopamine quinone + H2O
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.14.18.1 polyphenol oxidase responsible for browning reaction in demaged plant tissues and fruits Anethum graveolens
1.14.18.1 PPO
-
 Anethum graveolens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.14.18.1 10
-
 substrate-dependent, with catechol as a subsrate Anethum graveolens
1.14.18.1 10
-
 substrate-dependent, with chorogenic acid as a subsrate Anethum graveolens
1.14.18.1 55
-
 substrate-dependent, with dopamine as a subsrate Anethum graveolens

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
1.14.18.1
-
 85 the reaction is carried out at various temperatures and pH values with the substrates catechol, dopamine and chorogenic acid. PPO activity is measured at different temperatures in the range from 0 to 85°C using the 3 different substrates to determine the optimum temperature of the enzyme Anethum graveolens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.14.18.1 5.5
-
 with chorogenic acid as a substrate, phosphate buffer Anethum graveolens
1.14.18.1 6.5
-
 with catechol as a substrate, phosphate buffer. PPO activity is highest at pH 6.5, but decreases at similar rates at each of the pH values studied Anethum graveolens
1.14.18.1 8.5
-
 with dopamine as a substrate, Tris-HCl buffer Anethum graveolens

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.14.18.1 additional information
-
 the reaction is carried out at various temperatures and pH values with the substrates catechol, dopamine and chorogenic acid Anethum graveolens

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.14.18.1 0.022
-
 L-cysteine
-
 Anethum graveolens
1.14.18.1 0.0289
-
 L-cysteine
-
 Anethum graveolens
1.14.18.1 0.0292
-
 L-cysteine
-
 Anethum graveolens
1.14.18.1 0.0473
-
 ascorbic acid
-
 Anethum graveolens
1.14.18.1 0.0647
-
 ascorbic acid
-
 Anethum graveolens
1.14.18.1 0.0692
-
 ascorbic acid
-
 Anethum graveolens
1.14.18.1 0.0809
-
 glutathione
-
 Anethum graveolens
1.14.18.1 0.084
-
 glutathione
-
 Anethum graveolens
1.14.18.1 0.0897
-
 glutathione
-
 Anethum graveolens

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
1.14.18.1 0.0204
-
-
 Anethum graveolens L-cysteine
1.14.18.1 0.0325
-
-
 Anethum graveolens glutathione
1.14.18.1 0.0426
-
-
 Anethum graveolens ascorbic acid