Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Navarro-Martinez, M.D.; Cabezas-Herrera, J.; Garcia-Canovas, F.; Rodriguez-Lopez, J.N.
    Inhibition of Stenotrophomonas maltophilia dihydrofolate reductase by methotrexate: a single slow-binding process (2007), J. Enzyme Inhib. Med. Chem., 22, 377-382.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.5.1.3 methotrexate slow-binding inhibition due to the generation of a long-lived, slowly dissociating enzyme-NADPH-inhibitor complex. One-step inhibition mechanism Stenotrophomonas maltophilia

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.5.1.3 0.0018
-
 7,8-dihydrofolate 25°C, pH 7.4 Stenotrophomonas maltophilia
1.5.1.3 0.012
-
 NADPH 25°C, pH 7.4 Stenotrophomonas maltophilia

Organism

EC Number Organism UniProt Comment Textmining
1.5.1.3 Stenotrophomonas maltophilia
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.5.1.3
-
 Stenotrophomonas maltophilia

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.5.1.3 7,8-dihydrofolate + NADPH + H+
-
 Stenotrophomonas maltophilia 5,6,7,8-tetrahydrofolate + NADP+
-
 ?

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.5.1.3 123
-
 7,8-dihydrofolate 25°C, pH 7.4 Stenotrophomonas maltophilia

Cofactor

EC Number Cofactor Comment Organism Structure
1.5.1.3 NADPH
-
 Stenotrophomonas maltophilia

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.5.1.3 0.0000000289
-
 methotrexate 25°C Stenotrophomonas maltophilia