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Literature summary extracted from

  • Kamitaka, Y.; Tsujimura, S.; Kataoka, K.; Sakurai, T.; Ikeda, T.; Kano, K.
    Effects of axial ligand mutation of the type I copper site in bilirubin oxidase on direct electron transfer-type bioelectrocatalytic reduction of dioxygen (2007), J. Electroanal. Chem., 601, 119-124.
No PubMed abstract available

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.3.3.5 expressed in Pichia pastoris Albifimbria verrucaria

Protein Variants

EC Number Protein Variants Comment Organism
1.3.3.5 M467Q the enzymatic activity of the mutant is very low toward bilirubin but it works as a good catalyst in direct electron transfer-type bioelectrocatalytic reduction of dioxygen into water, the kcat value is 3fold increased Albifimbria verrucaria

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.3.3.5 0.0192
-
 K4[Fe(CN)6] wild type recombinant enzyme, at pH 7.0 and 25°C Albifimbria verrucaria
1.3.3.5 5.966
-
 K4[Fe(CN)6] mutant enzyme M467Q, at pH 7.0 and 25°C Albifimbria verrucaria

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.3.3.5 Cu2+ multi-copper oxidase Albifimbria verrucaria

Organism

EC Number Organism UniProt Comment Textmining
1.3.3.5 Albifimbria verrucaria
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.3.3.5
-
 Albifimbria verrucaria

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.3.3.5 bilirubin + O2
-
 Albifimbria verrucaria biliverdin + H2O
-
 ?
1.3.3.5 K4[Fe(CN)6] + O2
-
 Albifimbria verrucaria K3[Fe(CN)6] + H2O
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.3.3.5 BOD
-
 Albifimbria verrucaria

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.3.3.5 374
-
 K4[Fe(CN)6] wild type recombinant enzyme, at pH 7.0 and 25°C Albifimbria verrucaria
1.3.3.5 1259
-
 K4[Fe(CN)6] mutant enzyme M467Q, at pH 7.0 and 25°C Albifimbria verrucaria