Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Hong-Brown, L.Q.; Brown, C.R.; Huber, D.S.; Lang, C.H.
    Lopinavir impairs protein synthesis and induces eEF2 phosphorylation via the activation of AMP-activated protein kinase (2008), J. Cell. Biochem., 105, 814-823.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.7.11.20 Calmodulin required Mus musculus
2.7.11.20 additional information inhibition of eEF2 phosphorylation leads to increased activity of its upstream regulators AMP-activated protein kinase and eEF2 kinase, overview Mus musculus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.11.20 compound C AMPK inhibitor compound C blocks eEF2K and eEF2 phosphorylation Mus musculus
2.7.11.20 lopinavir i.e. LPV, increases the phosphorylation of eEF2 kinase on Ser366 reducing its activity, LPV affects eEF2 activity via an AMPK-eEF2K dependent pathway, overview Mus musculus
2.7.11.20 additional information rottlerin suppresses eEF2K, but not eEF2 phosphorylation in myocytes Mus musculus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.11.20 Ca2+ required Mus musculus
2.7.11.20 Mg2+ required Mus musculus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.11.20 ATP + [eukaryotic translation elongation factor 2] Mus musculus inhibition of eEF2 phosphorylation leads to increased activity of its upstream regulators AMP-activated protein kinase, AMPK, and eEF2 kinase, eEF2K, regulation, overview ADP + [eukaryotic translation elongation factor 2] phosphate
-
 ?
2.7.11.20 additional information Mus musculus rottlerin suppresses eEF2K, but not eEF2 phosphorylation in myocytes ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.11.20 Mus musculus
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
2.7.11.20 phosphoprotein phosphorylation of eEF2K can either inhibit or enhance the activity of its downstream substrate, depending on the site of phosphorylation and the type of stimul, lopinavir increases the phosphorylation of eEF2 kinase on Ser366 reducing its activity, LPV affects eEF2 activity via an AMPK-eEF2K dependent pathway, overview Mus musculus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.11.20 C2C12 cell murine myoblasts Mus musculus
-
2.7.11.20 myocyte
-
 Mus musculus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.11.20 additional information
-
-
 Mus musculus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.11.20 ATP + [eukaryotic translation elongation factor 2] inhibition of eEF2 phosphorylation leads to increased activity of its upstream regulators AMP-activated protein kinase, AMPK, and eEF2 kinase, eEF2K, regulation, overview Mus musculus ADP + [eukaryotic translation elongation factor 2] phosphate
-
 ?
2.7.11.20 ATP + [eukaryotic translation elongation factor 2] i.e. eEF2, both AMP-activated protein kinase, AMPK, and eEF2 kinase, eEF2K, phosphorylate eEF2, thus two distinct paths lead to eEF2 phosphorylation, overview Mus musculus ADP + [eukaryotic translation elongation factor 2] phosphate
-
 ?
2.7.11.20 additional information rottlerin suppresses eEF2K, but not eEF2 phosphorylation in myocytes Mus musculus ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.7.11.20 eEF2 kinase
-
 Mus musculus
2.7.11.20 eEF2K
-
 Mus musculus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.11.20 30
-
 assay at Mus musculus

Cofactor

EC Number Cofactor Comment Organism Structure
2.7.11.20 ATP
-
 Mus musculus