BRENDA - Enzyme Database show

Stat3 up-regulates expression of nicotinamide N-methyltransferase in human cancer cells

Tomida, M.; Ohtake, H.; Yokota, T.; Kobayashi, Y.; Kurosumi, M.; J. Cancer Res. Clin. Oncol. 134, 551-559 (2008)

Data extracted from this reference:

Application
EC Number
Application
Commentary
Organism
2.1.1.1
pharmacology
molecular targets for cancer therapy and diagnosis
Homo sapiens
Cloned(Commentary)
EC Number
Commentary
Organism
2.1.1.1
expressed in Escherichia coli, NNMT promoter region, promoter-luciferase reporter construct generated, pGL2 vector
Homo sapiens
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.1.1.1
Homo sapiens
P40261
-
-
Purification (Commentary)
EC Number
Commentary
Organism
2.1.1.1
recombinant protein, gel fitration
Homo sapiens
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
2.1.1.1
HEK-293 cell
no expression of NNMT in
Homo sapiens
-
2.1.1.1
Hep-G2 cell
NNMT expression increased on stimulation of the cells with interleukin 6, NNMT promoter activity dependent on the activation of Stat3
Homo sapiens
-
2.1.1.1
HT-29 cell
colon cancer cells with high and constitutive expression of NNMT
Homo sapiens
-
2.1.1.1
MCF-7 cell
no expression of NNMT in
Homo sapiens
-
2.1.1.1
MDA-MB-468 cell
breast cancer cells with high and constitutive expression of NNMT
Homo sapiens
-
2.1.1.1
SW-480 cell
colon cancer cells, NNMT expression increased on stimulation of the cells with interleukin 6
Homo sapiens
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
2.1.1.1
additional information
-
signal transducers and activators of transcription 3 (Stat3)-regulated genes analyzed, expression of NNMT in cancer cells, promoter activity determined after transfection experiments with NNMT promoter-luciferase reporter construct, real-time PCR, Western-Blot and immunohistochemistry, correlation between the expression of NNMT and activated Stat3 in colon cancer cells, NNMT determined as a Stat3-regulated gene and as a potential tumor marker
Homo sapiens
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.1.1.1
S-adenosyl-L-methionine + nicotinamide
NNMT as potential candidate for a tumor marker
687897
Homo sapiens
S-adenosyl-L-homocysteine + 1-methylnicotinamide
-
-
-
?
Application (protein specific)
EC Number
Application
Commentary
Organism
2.1.1.1
pharmacology
molecular targets for cancer therapy and diagnosis
Homo sapiens
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
2.1.1.1
expressed in Escherichia coli, NNMT promoter region, promoter-luciferase reporter construct generated, pGL2 vector
Homo sapiens
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
2.1.1.1
recombinant protein, gel fitration
Homo sapiens
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
2.1.1.1
HEK-293 cell
no expression of NNMT in
Homo sapiens
-
2.1.1.1
Hep-G2 cell
NNMT expression increased on stimulation of the cells with interleukin 6, NNMT promoter activity dependent on the activation of Stat3
Homo sapiens
-
2.1.1.1
HT-29 cell
colon cancer cells with high and constitutive expression of NNMT
Homo sapiens
-
2.1.1.1
MCF-7 cell
no expression of NNMT in
Homo sapiens
-
2.1.1.1
MDA-MB-468 cell
breast cancer cells with high and constitutive expression of NNMT
Homo sapiens
-
2.1.1.1
SW-480 cell
colon cancer cells, NNMT expression increased on stimulation of the cells with interleukin 6
Homo sapiens
-
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
2.1.1.1
additional information
-
signal transducers and activators of transcription 3 (Stat3)-regulated genes analyzed, expression of NNMT in cancer cells, promoter activity determined after transfection experiments with NNMT promoter-luciferase reporter construct, real-time PCR, Western-Blot and immunohistochemistry, correlation between the expression of NNMT and activated Stat3 in colon cancer cells, NNMT determined as a Stat3-regulated gene and as a potential tumor marker
Homo sapiens
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.1.1.1
S-adenosyl-L-methionine + nicotinamide
NNMT as potential candidate for a tumor marker
687897
Homo sapiens
S-adenosyl-L-homocysteine + 1-methylnicotinamide
-
-
-
?