BRENDA - Enzyme Database show

Expression in yeast of secreted lignin peroxidase with improved 2,4-dichlorophenol degradability by DNA shuffling

Ryu, K.; Kang, J.H.; Wang, L.; Lee, E.K.; J. Biotechnol. 135, 241-246 (2008)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Commentary
Organism
1.11.1.14
expression in Saccharomyces cerevisiae
Phanerochaete chrysosporium
Engineering
EC Number
Amino acid exchange
Commentary
Organism
1.11.1.14
A140G/S190P/P193A/S196F/E208Q
the variant shows increased 2,4-dichlorophenol degradation activity (ca. 1.6fold) and stability against H2O2. Kcat for H2O2 increases over the wild type value by about 6.5fold, the Km values for H2O2 is lower than wild type value
Phanerochaete chrysosporium
1.11.1.14
P106R/S119R/N120T/S228Y/A272G/L275V/A315G/A317T
the variant shows increased 2,4-dichlorophenol degradation activity (ca. 1.6fold) and stability against H2O2. Kcat for H2O2 increases over the wild type value by about 6.5fold, the Km values for H2O2 is lower than wild type value
Phanerochaete chrysosporium
1.11.1.14
S274L/L275F/A292
the variant shows increased 2,4-dichlorophenol degradation activity (ca. 1.6fold) and stability against H2O2. Kcat for H2O2 increases over the wild type value by about 6.5fold, the Km values for H2O2 is lower than wild type value
Phanerochaete chrysosporium
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.11.1.14
0.163
-
2,4-Dichlorophenol
mutant enzyme A140G/S190P/P193A/S196F/E208Q
Phanerochaete chrysosporium
1.11.1.14
0.179
-
2,4-Dichlorophenol
mutant enzyme S274L/L275F/A292
Phanerochaete chrysosporium
1.11.1.14
0.19
-
2,4-Dichlorophenol
mutant enzyme P106R/S119R/N120T/S228Y/A272G/L275V/A315G/A317T
Phanerochaete chrysosporium
1.11.1.14
0.359
-
2,4-Dichlorophenol
wild-type enzyme
Phanerochaete chrysosporium
1.11.1.14
4.09
-
H2O2
mutant enzyme P106R/S119R/N120T/S228Y/A272G/L275V/A315G/A317T
Phanerochaete chrysosporium
1.11.1.14
4.32
-
H2O2
mutant enzyme S274L/L275F/A292
Phanerochaete chrysosporium
1.11.1.14
4.47
-
H2O2
mutant enzyme A140G/S190P/P193A/S196F/E208Q
Phanerochaete chrysosporium
1.11.1.14
23.6
-
H2O2
wild-type enzyme
Phanerochaete chrysosporium
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.11.1.14
Phanerochaete chrysosporium
-
-
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.11.1.14
2,4-dichlorophenol + H2O2
-
687889
Phanerochaete chrysosporium
?
-
-
-
?
Turnover Number [1/s]
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
1.11.1.14
10.83
-
H2O2
wild-type enzyme
Phanerochaete chrysosporium
1.11.1.14
37.1
-
2,4-Dichlorophenol
wild-type enzyme
Phanerochaete chrysosporium
1.11.1.14
58.7
-
2,4-Dichlorophenol
mutant enzyme A140G/S190P/P193A/S196F/E208Q
Phanerochaete chrysosporium
1.11.1.14
59.7
-
2,4-Dichlorophenol
mutant enzyme S274L/L275F/A292
Phanerochaete chrysosporium
1.11.1.14
66
-
2,4-Dichlorophenol
mutant enzyme P106R/S119R/N120T/S228Y/A272G/L275V/A315G/A317T
Phanerochaete chrysosporium
1.11.1.14
68.2
-
H2O2
mutant enzyme P106R/S119R/N120T/S228Y/A272G/L275V/A315G/A317T
Phanerochaete chrysosporium
1.11.1.14
72
-
H2O2
mutant enzyme S274L/L275F/A292
Phanerochaete chrysosporium
1.11.1.14
74.5
-
H2O2
mutant enzyme A140G/S190P/P193A/S196F/E208Q
Phanerochaete chrysosporium
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
1.11.1.14
expression in Saccharomyces cerevisiae
Phanerochaete chrysosporium
Engineering (protein specific)
EC Number
Amino acid exchange
Commentary
Organism
1.11.1.14
A140G/S190P/P193A/S196F/E208Q
the variant shows increased 2,4-dichlorophenol degradation activity (ca. 1.6fold) and stability against H2O2. Kcat for H2O2 increases over the wild type value by about 6.5fold, the Km values for H2O2 is lower than wild type value
Phanerochaete chrysosporium
1.11.1.14
P106R/S119R/N120T/S228Y/A272G/L275V/A315G/A317T
the variant shows increased 2,4-dichlorophenol degradation activity (ca. 1.6fold) and stability against H2O2. Kcat for H2O2 increases over the wild type value by about 6.5fold, the Km values for H2O2 is lower than wild type value
Phanerochaete chrysosporium
1.11.1.14
S274L/L275F/A292
the variant shows increased 2,4-dichlorophenol degradation activity (ca. 1.6fold) and stability against H2O2. Kcat for H2O2 increases over the wild type value by about 6.5fold, the Km values for H2O2 is lower than wild type value
Phanerochaete chrysosporium
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.11.1.14
0.163
-
2,4-Dichlorophenol
mutant enzyme A140G/S190P/P193A/S196F/E208Q
Phanerochaete chrysosporium
1.11.1.14
0.179
-
2,4-Dichlorophenol
mutant enzyme S274L/L275F/A292
Phanerochaete chrysosporium
1.11.1.14
0.19
-
2,4-Dichlorophenol
mutant enzyme P106R/S119R/N120T/S228Y/A272G/L275V/A315G/A317T
Phanerochaete chrysosporium
1.11.1.14
0.359
-
2,4-Dichlorophenol
wild-type enzyme
Phanerochaete chrysosporium
1.11.1.14
4.09
-
H2O2
mutant enzyme P106R/S119R/N120T/S228Y/A272G/L275V/A315G/A317T
Phanerochaete chrysosporium
1.11.1.14
4.32
-
H2O2
mutant enzyme S274L/L275F/A292
Phanerochaete chrysosporium
1.11.1.14
4.47
-
H2O2
mutant enzyme A140G/S190P/P193A/S196F/E208Q
Phanerochaete chrysosporium
1.11.1.14
23.6
-
H2O2
wild-type enzyme
Phanerochaete chrysosporium
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.11.1.14
2,4-dichlorophenol + H2O2
-
687889
Phanerochaete chrysosporium
?
-
-
-
?
Turnover Number [1/s] (protein specific)
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
1.11.1.14
10.83
-
H2O2
wild-type enzyme
Phanerochaete chrysosporium
1.11.1.14
37.1
-
2,4-Dichlorophenol
wild-type enzyme
Phanerochaete chrysosporium
1.11.1.14
58.7
-
2,4-Dichlorophenol
mutant enzyme A140G/S190P/P193A/S196F/E208Q
Phanerochaete chrysosporium
1.11.1.14
59.7
-
2,4-Dichlorophenol
mutant enzyme S274L/L275F/A292
Phanerochaete chrysosporium
1.11.1.14
66
-
2,4-Dichlorophenol
mutant enzyme P106R/S119R/N120T/S228Y/A272G/L275V/A315G/A317T
Phanerochaete chrysosporium
1.11.1.14
68.2
-
H2O2
mutant enzyme P106R/S119R/N120T/S228Y/A272G/L275V/A315G/A317T
Phanerochaete chrysosporium
1.11.1.14
72
-
H2O2
mutant enzyme S274L/L275F/A292
Phanerochaete chrysosporium
1.11.1.14
74.5
-
H2O2
mutant enzyme A140G/S190P/P193A/S196F/E208Q
Phanerochaete chrysosporium