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Literature summary extracted from

  • Ryu, K.; Kang, J.H.; Wang, L.; Lee, E.K.
    Expression in yeast of secreted lignin peroxidase with improved 2,4-dichlorophenol degradability by DNA shuffling (2008), J. Biotechnol., 135, 241-246.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.11.1.14 expression in Saccharomyces cerevisiae Phanerodontia chrysosporium

Protein Variants

EC Number Protein Variants Comment Organism
1.11.1.14 A140G/S190P/P193A/S196F/E208Q the variant shows increased 2,4-dichlorophenol degradation activity (ca. 1.6fold) and stability against H2O2. Kcat for H2O2 increases over the wild type value by about 6.5fold, the Km values for H2O2 is lower than wild type value Phanerodontia chrysosporium
1.11.1.14 P106R/S119R/N120T/S228Y/A272G/L275V/A315G/A317T the variant shows increased 2,4-dichlorophenol degradation activity (ca. 1.6fold) and stability against H2O2. Kcat for H2O2 increases over the wild type value by about 6.5fold, the Km values for H2O2 is lower than wild type value Phanerodontia chrysosporium
1.11.1.14 S274L/L275F/A292 the variant shows increased 2,4-dichlorophenol degradation activity (ca. 1.6fold) and stability against H2O2. Kcat for H2O2 increases over the wild type value by about 6.5fold, the Km values for H2O2 is lower than wild type value Phanerodontia chrysosporium

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.11.1.14 0.163
-
 2,4-Dichlorophenol mutant enzyme A140G/S190P/P193A/S196F/E208Q Phanerodontia chrysosporium
1.11.1.14 0.179
-
 2,4-Dichlorophenol mutant enzyme S274L/L275F/A292 Phanerodontia chrysosporium
1.11.1.14 0.19
-
 2,4-Dichlorophenol mutant enzyme P106R/S119R/N120T/S228Y/A272G/L275V/A315G/A317T Phanerodontia chrysosporium
1.11.1.14 0.359
-
 2,4-Dichlorophenol wild-type enzyme Phanerodontia chrysosporium
1.11.1.14 4.09
-
 H2O2 mutant enzyme P106R/S119R/N120T/S228Y/A272G/L275V/A315G/A317T Phanerodontia chrysosporium
1.11.1.14 4.32
-
 H2O2 mutant enzyme S274L/L275F/A292 Phanerodontia chrysosporium
1.11.1.14 4.47
-
 H2O2 mutant enzyme A140G/S190P/P193A/S196F/E208Q Phanerodontia chrysosporium
1.11.1.14 23.6
-
 H2O2 wild-type enzyme Phanerodontia chrysosporium

Organism

EC Number Organism UniProt Comment Textmining
1.11.1.14 Phanerodontia chrysosporium
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.11.1.14 2,4-dichlorophenol + H2O2
-
 Phanerodontia chrysosporium ?
-
 ?

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.11.1.14 10.83
-
 H2O2 wild-type enzyme Phanerodontia chrysosporium
1.11.1.14 37.1
-
 2,4-Dichlorophenol wild-type enzyme Phanerodontia chrysosporium
1.11.1.14 58.7
-
 2,4-Dichlorophenol mutant enzyme A140G/S190P/P193A/S196F/E208Q Phanerodontia chrysosporium
1.11.1.14 59.7
-
 2,4-Dichlorophenol mutant enzyme S274L/L275F/A292 Phanerodontia chrysosporium
1.11.1.14 66
-
 2,4-Dichlorophenol mutant enzyme P106R/S119R/N120T/S228Y/A272G/L275V/A315G/A317T Phanerodontia chrysosporium
1.11.1.14 68.2
-
 H2O2 mutant enzyme P106R/S119R/N120T/S228Y/A272G/L275V/A315G/A317T Phanerodontia chrysosporium
1.11.1.14 72
-
 H2O2 mutant enzyme S274L/L275F/A292 Phanerodontia chrysosporium
1.11.1.14 74.5
-
 H2O2 mutant enzyme A140G/S190P/P193A/S196F/E208Q Phanerodontia chrysosporium