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Literature summary extracted from

  • Grey, C.E.; Rundbaeck, F.; Adlercreutz, P.
    Improved operational stability of chloroperoxidase through use of antioxidants (2008), J. Biotechnol., 135, 196-201.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.11.1.10 ascorbic acid enhances total turnover number compared to reaction without antioxidant Leptoxyphium fumago
1.11.1.10 caffeic acid the total turnover number is almost 5fold higher than that obtained for the reference reaction Leptoxyphium fumago
1.11.1.10 citraconic anhydride the catalytic efficiency of the modified enzyme for sulfoxidation in aqueous buffer is increased by 26.2% Leptoxyphium fumago
1.11.1.10 ferulic acid enhances total turnover number compared to reaction without antioxidant Leptoxyphium fumago
1.11.1.10 Maleic anhydride the catalytic efficiency of the modified enzyme for sulfoxidation in aqueous buffer is increased by 22.6% Leptoxyphium fumago
1.11.1.10 phthalic anhydride the catalytic efficiency of the modified enzyme for sulfoxidation in aqueous buffer is increased by 12.9% Leptoxyphium fumago

General Stability

EC Number General Stability Organism
1.11.1.10 the thermostability of peroxidase of CPO is increased about 2fold upon these chemical modification by citraconic anhydride, phthalic anhydride or maleic anhydride. The thermostability of sulfoxidation activity of CPO is increased about 1.2fold upon the chemical modification by citraconic anhydride, phthalic anhydride or maleic anhydride Leptoxyphium fumago

Organism

EC Number Organism UniProt Comment Textmining
1.11.1.10 Leptoxyphium fumago
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.11.1.10 indole + H2O2
-
 Leptoxyphium fumago 2-oxoindole + H2O
-
 ?
1.11.1.10 indole + tert-butyl hydroperoxide
-
 Leptoxyphium fumago 2-oxoindole + ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.11.1.10 chloroperoxidase
-
 Leptoxyphium fumago
1.11.1.10 CPO
-
 Leptoxyphium fumago

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.11.1.10 additional information
-
 the thermostability of peroxidase of CPO is increased about 2fold upon these chemical modification by citraconic anhydride, phthalic anhydride or maleic anhydride. The thermostability of sulfoxidation activity of CPO is increased about 1.2fold upon these chemical modification by citraconic anhydride, phthalic anhydride or maleic anhydride Leptoxyphium fumago
1.11.1.10 50
-
 6 h, native CPO retains about 10% peroxidase activity and 20% sulfoxidation activity in aqueous buffer. CPOs modified by citraconic anhydride, phthalic anhydride or maleic anhydride, retain about 30% peroxidase activity and 30% sulfoxidation activity Leptoxyphium fumago