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Literature summary extracted from

  • Sygmund, C.; Kittl, R.; Volc, J.; Halada, P.; Kubatova, E.; Haltrich, D.; Peterbauer, C.K.
    Characterization of pyranose dehydrogenase from Agaricus meleagris and its application in the C-2 specific conversion of D-galactose (2008), J. Biotechnol., 133, 334-342.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.1.99.29 NH4+ at 0.1-1 M in 20 mM sodium phosphate buffer pH 7.0, not due to increased ionic strenth Leucoagaricus meleagris

Application

EC Number Application Comment Organism
1.1.99.29 degradation lignocellulose degradation Leucoagaricus meleagris

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.99.29 alpha,alpha-dipyridyl no activity Leucoagaricus meleagris
1.1.99.29 CN- no activity Leucoagaricus meleagris
1.1.99.29 EDTA no activity Leucoagaricus meleagris
1.1.99.29 F- no activity Leucoagaricus meleagris
1.1.99.29 MoO42- no activity Leucoagaricus meleagris
1.1.99.29 NaN3 no activity Leucoagaricus meleagris
1.1.99.29 NH4+ at 2 M in 20 mM sodium phosphate buffer pH 7.0 Leucoagaricus meleagris
1.1.99.29 p-hydroxymercuribenzoate no activity Leucoagaricus meleagris
1.1.99.29 Phenanthroline no activity Leucoagaricus meleagris

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.99.29 0.13
-
Fe3+ at 30°C with 50 mM glucose as susbtrate Leucoagaricus meleagris
1.1.99.29 0.18
-
methyl-1,4-benzoquinone at 30°C with 50 mM glucose as susbtrate Leucoagaricus meleagris
1.1.99.29 0.22
-
3,5-di-tert-butyl-1,4-benzoquinone at 30°C with 50 mM glucose as susbtrate Leucoagaricus meleagris
1.1.99.29 0.54
-
L-arabinose Fe3+ as electron acceptor Leucoagaricus meleagris
1.1.99.29 0.55
-
2-chloro-1,4-benzoquinone at 30°C with 50 mM glucose as susbtrate Leucoagaricus meleagris
1.1.99.29 0.82
-
D-glucose Fe3+ as electron acceptor Leucoagaricus meleagris
1.1.99.29 0.92
-
2,5-chloro-1,4-benzoquinone at 30°C with 50 mM glucose as susbtrate Leucoagaricus meleagris
1.1.99.29 1.05
-
D-galactose Fe3+ as electron acceptor Leucoagaricus meleagris
1.1.99.29 1.82
-
1,4-benzoquinone at 30°C with 50 mM glucose as susbtrate Leucoagaricus meleagris
1.1.99.29 1.93
-
D-xylose Fe3+ as electron acceptor Leucoagaricus meleagris
1.1.99.29 6.82
-
cellobiose Fe3+ as electron acceptor Leucoagaricus meleagris
1.1.99.29 8.61
-
D-maltose Fe3+ as electron acceptor Leucoagaricus meleagris
1.1.99.29 10.8
-
D-xylobiose Fe3+ as electron acceptor Leucoagaricus meleagris
1.1.99.29 59.7
-
D-ribose Fe3+ as electron acceptor Leucoagaricus meleagris
1.1.99.29 79.1
-
D-talose Fe3+ as electron acceptor Leucoagaricus meleagris
1.1.99.29 108
-
D-mannose Fe3+ as electron acceptor Leucoagaricus meleagris
1.1.99.29 134
-
lactose Fe3+ as electron acceptor Leucoagaricus meleagris
1.1.99.29 137
-
L-sorbose Fe3+ as electron acceptor Leucoagaricus meleagris
1.1.99.29 153
-
D-fructose Fe3+ as electron acceptor Leucoagaricus meleagris
1.1.99.29 156
-
maltotriose Fe3+ as electron acceptor Leucoagaricus meleagris

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.1.99.29 extracellular
-
Leucoagaricus meleagris
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.1.99.29 Ag+ 37% inhibition of activity Leucoagaricus meleagris
1.1.99.29 Al3+ no activity Leucoagaricus meleagris
1.1.99.29 Ca2+ no activity Leucoagaricus meleagris
1.1.99.29 Cu2+ no activity Leucoagaricus meleagris
1.1.99.29 Fe2+ no activity Leucoagaricus meleagris
1.1.99.29 Fe3+ 39% inhibition of activity Leucoagaricus meleagris
1.1.99.29 Hg2+ 25% inhibition of activity Leucoagaricus meleagris
1.1.99.29 Mg2+ no activity Leucoagaricus meleagris
1.1.99.29 Mn2+ no activity Leucoagaricus meleagris
1.1.99.29 Ni2+ no activity Leucoagaricus meleagris
1.1.99.29 Zn2+ no activity Leucoagaricus meleagris

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.99.29 46000
-
SDS-PAGE, PDHf, second band Leucoagaricus meleagris
1.1.99.29 66500
-
SDS-PAGE, gel filtration, MALDI MS Leucoagaricus meleagris

Organism

EC Number Organism UniProt Comment Textmining
1.1.99.29 Leucoagaricus meleagris
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
1.1.99.29 glycoprotein carbohydrate content of 7% Leucoagaricus meleagris

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.99.29 using DEAE-Sephacel anion exchange chromatography and FPLC on phenyl-superose HR 10/10, Mono Q HR 5/5 and superose 12 HR 30/10 chromatography Leucoagaricus meleagris

Source Tissue

EC Number Source Tissue Comment Organism Textmining

Storage Stability

EC Number Storage Stability Organism
1.1.99.29 stable for 3 months at 4°C, in sodium phosphate buffer pH 7.0 Leucoagaricus meleagris

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.99.29 cellobiose + Fe3+
-
Leucoagaricus meleagris ?
-
?
1.1.99.29 D-fructose + Fe3+
-
Leucoagaricus meleagris ?
-
?
1.1.99.29 D-galactose + benzoquinone
-
Leucoagaricus meleagris 2-dehydro-D-galactose + hydroquinone no 2,3-didehydro-d-galactose is detectable ?
1.1.99.29 D-galactose + Fe3+
-
Leucoagaricus meleagris 2-dehydro-D-galactose + Fe2+ + H+
-
?
1.1.99.29 D-glucose pH 8.5 Leucoagaricus meleagris 3-dehydro-D-glucose + Fe2+ + H+
-
?
1.1.99.29 D-glucose + 2,5-chloro-1,4-benzoquinone pH 5 Leucoagaricus meleagris ?
-
?
1.1.99.29 D-glucose + 2,6-dichloroindophenol pH 4 Leucoagaricus meleagris ?
-
?
1.1.99.29 D-glucose + 2-chloro-1,4-benzoquinone pH 5 Leucoagaricus meleagris ?
-
?
1.1.99.29 D-glucose + 3,5-di-tert-butyl-1,4-benzoquinone pH 6 Leucoagaricus meleagris ?
-
?
1.1.99.29 D-glucose + benzoquinone pH 3 Leucoagaricus meleagris 2,3-didehydro-D-glucose + hydroquinone
-
?
1.1.99.29 D-glucose + methyl-1,4-benzoquinone pH 8 Leucoagaricus meleagris ?
-
?
1.1.99.29 D-maltose + Fe3+
-
Leucoagaricus meleagris ?
-
?
1.1.99.29 D-maltotriose + Fe3+
-
Leucoagaricus meleagris ?
-
?
1.1.99.29 D-mannose + Fe3+
-
Leucoagaricus meleagris ?
-
?
1.1.99.29 D-ribose + Fe3+
-
Leucoagaricus meleagris ?
-
?
1.1.99.29 D-talose + Fe3+
-
Leucoagaricus meleagris ?
-
?
1.1.99.29 D-xylobiose + ferricenium ion
-
Leucoagaricus meleagris ?
-
r
1.1.99.29 D-xylose + Fe3+
-
Leucoagaricus meleagris ?
-
?
1.1.99.29 L-arabinose + Fe3+
-
Leucoagaricus meleagris ?
-
?
1.1.99.29 L-sorbose + Fe3+
-
Leucoagaricus meleagris ?
-
?
1.1.99.29 L-xylobiose + Fe3+
-
Leucoagaricus meleagris ?
-
?
1.1.99.29 lactose + Fe3+
-
Leucoagaricus meleagris ?
-
?
1.1.99.29 maltotriose + ferricenium ion
-
Leucoagaricus meleagris ?
-
r

Subunits

EC Number Subunits Comment Organism
1.1.99.29 monomer gel filtration Leucoagaricus meleagris

Synonyms

EC Number Synonyms Comment Organism
1.1.99.29 PDH
-
Leucoagaricus meleagris
1.1.99.29 pyranose dehydrogenase
-
Leucoagaricus meleagris
1.1.99.29 pyranose:acceptor oxidoreductase
-
Leucoagaricus meleagris

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.99.29 63
-
-
Leucoagaricus meleagris

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.1.99.29 50
-
50% activity loss after incubation at 50°C for 50 h Leucoagaricus meleagris

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.99.29 2
-
for glucose and 1,4-benzoquinone as substrates Leucoagaricus meleagris
1.1.99.29 9
-
Fe3+ as electron acceptor, with bisTris propane buffer Leucoagaricus meleagris

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.1.99.29 2 10 depending on the electron acceptor and with d-glucose as electron donor, has 2 pH optima for some substrates Leucoagaricus meleagris

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.1.99.29 7
-
80% maximum stability at pH 7 Leucoagaricus meleagris

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.99.29 FAD 5 FAD covalently bound Leucoagaricus meleagris

pI Value

EC Number Organism Comment pI Value Maximum pI Value
1.1.99.29 Leucoagaricus meleagris isoelectric focusing, 4 isoforms
-
4.2
1.1.99.29 Leucoagaricus meleagris isoelectric focusing, 4 isoforms
-
4.25
1.1.99.29 Leucoagaricus meleagris isoelectric focusing, 4 isoforms
-
4.35
1.1.99.29 Leucoagaricus meleagris isoelectric focusing, 4 isoforms
-
4.45