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Functionality improvement of fungal lignin peroxidase by DNA shuffling for 2,4-dichlorophenol degradability and H2O2 stability

Ryu, K.; Hwang, S.Y.; Kim, K.H.; Kang, J.H.; Lee, E.K.; J. Biotechnol. 133, 110-115 (2008)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Commentary
Organism
1.11.1.14
expression in Saccharomyces cerevisiae
Phanerochaete chrysosporium
Engineering
EC Number
Amino acid exchange
Commentary
Organism
1.11.1.14
A140G/A243R/A317P
kcat/Km for 2,4-dichlorophenol is 4fold higher than wild-type value, kcat/Km for H2O2 is 89fold higher than wild-type value
Phanerochaete chrysosporium
1.11.1.14
H102T/S119R/N120T/Q126K/A243R/A315G
kcat/Km for 2,4-dichlorophenol is fold higher than wild-type value, kcat/Km for H2O2 is 89fold higher than wild-type value
Phanerochaete chrysosporium
1.11.1.14
P106R/Q210H/L211V/A243R/F255L
kcat/Km for 2,4-dichlorophenol is 4fold higher than wild-type value, kcat/Km for H2O2 is 89fold higher than wild-type value
Phanerochaete chrysosporium
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.11.1.14
0.359
-
2,4-Dichlorophenol
wild-type enzyme
Phanerochaete chrysosporium
1.11.1.14
0.74
-
2,4-Dichlorophenol
mutant enzyme H102T/S119R/N120T/Q126K/A243R/A315G
Phanerochaete chrysosporium
1.11.1.14
0.755
-
2,4-Dichlorophenol
mutant enzyme P106R/Q210H/L211V/A243R/F255L
Phanerochaete chrysosporium
1.11.1.14
0.79
-
2,4-Dichlorophenol
mutant enzyme A140G/A243R/A317P
Phanerochaete chrysosporium
1.11.1.14
4.08
-
H2O2
mutant enzyme P106R/Q210H/L211V/A243R/F255L
Phanerochaete chrysosporium
1.11.1.14
4.21
-
H2O2
mutant enzyme A140G/A243R/A317P
Phanerochaete chrysosporium
1.11.1.14
4.24
-
H2O2
mutant enzyme H102T/S119R/N120T/Q126K/A243R/A315G
Phanerochaete chrysosporium
1.11.1.14
23.6
-
H2O2
wild-type enzyme
Phanerochaete chrysosporium
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.11.1.14
Phanerochaete chrysosporium
-
-
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.11.1.14
2,4-dichlorophenol + H2O2
-
687879
Phanerochaete chrysosporium
?
-
-
-
?
Turnover Number [1/s]
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
1.11.1.14
10.83
-
H2O2
wild-type enzyme
Phanerochaete chrysosporium
1.11.1.14
37.2
-
2,4-Dichlorophenol
wild-type enzyme
Phanerochaete chrysosporium
1.11.1.14
171.7
-
H2O2
mutant enzyme P106R/Q210H/L211V/A243R/F255L
Phanerochaete chrysosporium
1.11.1.14
175
-
H2O2
mutant enzyme A140G/A243R/A317P
Phanerochaete chrysosporium
1.11.1.14
176.7
-
H2O2
mutant enzyme H102T/S119R/N120T/Q126K/A243R/A315G
Phanerochaete chrysosporium
1.11.1.14
313.3
-
2,4-Dichlorophenol
mutant enzyme H102T/S119R/N120T/Q126K/A243R/A315G
Phanerochaete chrysosporium
1.11.1.14
316.7
-
2,4-Dichlorophenol
mutant enzyme P106R/Q210H/L211V/A243R/F255L
Phanerochaete chrysosporium
1.11.1.14
333.3
-
2,4-Dichlorophenol
mutant enzyme A140G/A243R/A317P
Phanerochaete chrysosporium
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
1.11.1.14
expression in Saccharomyces cerevisiae
Phanerochaete chrysosporium
Engineering (protein specific)
EC Number
Amino acid exchange
Commentary
Organism
1.11.1.14
A140G/A243R/A317P
kcat/Km for 2,4-dichlorophenol is 4fold higher than wild-type value, kcat/Km for H2O2 is 89fold higher than wild-type value
Phanerochaete chrysosporium
1.11.1.14
H102T/S119R/N120T/Q126K/A243R/A315G
kcat/Km for 2,4-dichlorophenol is fold higher than wild-type value, kcat/Km for H2O2 is 89fold higher than wild-type value
Phanerochaete chrysosporium
1.11.1.14
P106R/Q210H/L211V/A243R/F255L
kcat/Km for 2,4-dichlorophenol is 4fold higher than wild-type value, kcat/Km for H2O2 is 89fold higher than wild-type value
Phanerochaete chrysosporium
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.11.1.14
0.359
-
2,4-Dichlorophenol
wild-type enzyme
Phanerochaete chrysosporium
1.11.1.14
0.74
-
2,4-Dichlorophenol
mutant enzyme H102T/S119R/N120T/Q126K/A243R/A315G
Phanerochaete chrysosporium
1.11.1.14
0.755
-
2,4-Dichlorophenol
mutant enzyme P106R/Q210H/L211V/A243R/F255L
Phanerochaete chrysosporium
1.11.1.14
0.79
-
2,4-Dichlorophenol
mutant enzyme A140G/A243R/A317P
Phanerochaete chrysosporium
1.11.1.14
4.08
-
H2O2
mutant enzyme P106R/Q210H/L211V/A243R/F255L
Phanerochaete chrysosporium
1.11.1.14
4.21
-
H2O2
mutant enzyme A140G/A243R/A317P
Phanerochaete chrysosporium
1.11.1.14
4.24
-
H2O2
mutant enzyme H102T/S119R/N120T/Q126K/A243R/A315G
Phanerochaete chrysosporium
1.11.1.14
23.6
-
H2O2
wild-type enzyme
Phanerochaete chrysosporium
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.11.1.14
2,4-dichlorophenol + H2O2
-
687879
Phanerochaete chrysosporium
?
-
-
-
?
Turnover Number [1/s] (protein specific)
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
1.11.1.14
10.83
-
H2O2
wild-type enzyme
Phanerochaete chrysosporium
1.11.1.14
37.2
-
2,4-Dichlorophenol
wild-type enzyme
Phanerochaete chrysosporium
1.11.1.14
171.7
-
H2O2
mutant enzyme P106R/Q210H/L211V/A243R/F255L
Phanerochaete chrysosporium
1.11.1.14
175
-
H2O2
mutant enzyme A140G/A243R/A317P
Phanerochaete chrysosporium
1.11.1.14
176.7
-
H2O2
mutant enzyme H102T/S119R/N120T/Q126K/A243R/A315G
Phanerochaete chrysosporium
1.11.1.14
313.3
-
2,4-Dichlorophenol
mutant enzyme H102T/S119R/N120T/Q126K/A243R/A315G
Phanerochaete chrysosporium
1.11.1.14
316.7
-
2,4-Dichlorophenol
mutant enzyme P106R/Q210H/L211V/A243R/F255L
Phanerochaete chrysosporium
1.11.1.14
333.3
-
2,4-Dichlorophenol
mutant enzyme A140G/A243R/A317P
Phanerochaete chrysosporium