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Literature summary extracted from

  • Kristan, K.; Stojan, J.; Adamski, J.; Lanisnik Rizner, T.
    Rational design of novel mutants of fungal 17beta-hydroxysteroid dehydrogenase (2007), J. Biotechnol., 129, 123-130.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.62 expressed as GST-fusion proteins in Escherichia coli JM107 cells Curvularia lunata

Protein Variants

EC Number Protein Variants Comment Organism
1.1.1.62 H164G mutation results in a conformation of the enzyme that possesses a higher catalytic activity Curvularia lunata
1.1.1.62 H164W mutation results in a conformation of the enzyme that possesses a higher catalytic activity Curvularia lunata
1.1.1.62 V161G mutation increases the initial rates for the conversion of androstenedione to testosterone Curvularia lunata
1.1.1.62 Y212A mutation increases the initial rates for the conversion of androstenedione to testosterone Curvularia lunata
1.1.1.62 Y49D mutation changes the coenzyme specificity of the enzyme. The mutant can convert the steroids not only in the presence of NADP+ and NADPH, but also in the presence of NADH and NAD+ Curvularia lunata

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.62 0.000088
-
NADP+ 25°C, pH 8.0, wild-type enzyme, cosubstrate: 4-estrene-17beta-ol-3-one Curvularia lunata
1.1.1.62 0.00019
-
NADP+ 25°C, pH 8.0, mutant enzyme H164W, cosubstrate: 4-estrene-17beta-ol-3-one Curvularia lunata
1.1.1.62 0.00028
-
NADP+ 25°C, pH 8.0, mutant enzyme H164G, cosubstrate: 4-estrene-17beta-ol-3-one Curvularia lunata
1.1.1.62 0.00035
-
NADPH 25°C, pH 8.0, mutant enzyme H164G, cosubstrate: 4-estrene-3,17-dione Curvularia lunata
1.1.1.62 0.0004
-
NADPH 25°C, pH 8.0, mutant enzyme H164W, cosubstrate: 4-estrene-3,17-dione Curvularia lunata
1.1.1.62 0.0065
-
NADPH 25°C, pH 8.0, wild-type enzyme, cosubstrate: 4-estrene-3,17-dione Curvularia lunata
1.1.1.62 0.16
-
NADPH 25°C, pH 8.0, mutant enzyme Y49D, cosubstrate: 4-estrene-3,17-dione Curvularia lunata
1.1.1.62 0.5
-
NAD+ 25°C, pH 8.0, mutant enzyme Y49D, cosubstrate: 4-estrene-17beta-ol-3-one Curvularia lunata
1.1.1.62 0.86
-
NADH 25°C, pH 8.0, mutant enzyme Y49D, cosubstrate: 4-estrene-3,17-dione Curvularia lunata
1.1.1.62 9
-
NADP+ 25°C, pH 8.0, mutant enzyme Y49D, cosubstrate: 4-estrene-17beta-ol-3-one Curvularia lunata

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.62 Curvularia lunata O93874
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.62 4-estrene-3,17-dione + NADH + H+
-
Curvularia lunata 4-estrene-17beta-ol-3-one + NAD+
-
r
1.1.1.62 4-estrene-3,17-dione + NADPH + H+
-
Curvularia lunata 4-estrene-17beta-ol-3-one + NADP+
-
r
1.1.1.62 5alpha-androstane-3,17-dione + NADH + H+
-
Curvularia lunata 5alpha-androstane-17beta-ol-3-one + NAD+
-
r
1.1.1.62 androst-4-ene-3,17-dione + NADH + H+
-
Curvularia lunata testosterone + NAD+
-
r
1.1.1.62 estrone + NADH + H+
-
Curvularia lunata estradiol + NAD+
-
r

Synonyms

EC Number Synonyms Comment Organism
1.1.1.62 17beta-HSDcl
-
Curvularia lunata
1.1.1.62 17beta-hydroxysteroid dehydrogenase
-
Curvularia lunata

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.1.1.62 0.065
-
NADPH 25°C, pH 8.0, mutant enzyme Y49D, cosubstrate: 4-estrene-3,17-dione Curvularia lunata
1.1.1.62 0.24
-
NAD+ 25°C, pH 8.0, mutant enzyme Y49D, cosubstrate: 4-estrene-17beta-ol-3-one Curvularia lunata
1.1.1.62 0.27
-
NADH 25°C, pH 8.0, mutant enzyme Y49D, cosubstrate: 4-estrene-3,17-dione Curvularia lunata
1.1.1.62 0.54
-
NADP+ 25°C, pH 8.0, mutant enzyme Y49D, cosubstrate: 4-estrene-17beta-ol-3-one Curvularia lunata
1.1.1.62 0.65
-
NADP+ 25°C, pH 8.0, wild-type enzyme, cosubstrate: 4-estrene-17beta-ol-3-one Curvularia lunata
1.1.1.62 1.8
-
NADPH 25°C, pH 8.0, mutant enzyme H164W, cosubstrate: 4-estrene-3,17-dione Curvularia lunata
1.1.1.62 1.9
-
NADPH 25°C, pH 8.0, mutant enzyme H164G, cosubstrate: 4-estrene-3,17-dione Curvularia lunata
1.1.1.62 3.1
-
NADP+ 25°C, pH 8.0, mutant enzyme H164W, cosubstrate: 4-estrene-17beta-ol-3-one Curvularia lunata
1.1.1.62 3.8
-
NADPH 25°C, pH 8.0, wild-type enzyme, cosubstrate: 4-estrene-3,17-dione Curvularia lunata
1.1.1.62 4.7
-
NADP+ 25°C, pH 8.0, mutant enzyme H164G, cosubstrate: 4-estrene-17beta-ol-3-one Curvularia lunata

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.62 NAD+ wild-type enzyme shows no activity with NADP+ Curvularia lunata
1.1.1.62 NADH wild-type enzyme shows no activity with NADPH Curvularia lunata
1.1.1.62 NADP+ mutant enzyme Y49D show activity with NADP+, wild-type enzyme shows no activity Curvularia lunata
1.1.1.62 NADPH mutant enzyme Y49D show activity with NADPH, wild-type enzyme shows no activity Curvularia lunata