EC Number | Cloned (Comment) | Organism |
---|---|---|
1.1.1.62 | expressed as GST-fusion proteins in Escherichia coli JM107 cells | Curvularia lunata |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
1.1.1.62 | H164G | mutation results in a conformation of the enzyme that possesses a higher catalytic activity | Curvularia lunata |
1.1.1.62 | H164W | mutation results in a conformation of the enzyme that possesses a higher catalytic activity | Curvularia lunata |
1.1.1.62 | V161G | mutation increases the initial rates for the conversion of androstenedione to testosterone | Curvularia lunata |
1.1.1.62 | Y212A | mutation increases the initial rates for the conversion of androstenedione to testosterone | Curvularia lunata |
1.1.1.62 | Y49D | mutation changes the coenzyme specificity of the enzyme. The mutant can convert the steroids not only in the presence of NADP+ and NADPH, but also in the presence of NADH and NAD+ | Curvularia lunata |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.1.1.62 | 0.000088 | - |
NADP+ | 25°C, pH 8.0, wild-type enzyme, cosubstrate: 4-estrene-17beta-ol-3-one | Curvularia lunata | |
1.1.1.62 | 0.00019 | - |
NADP+ | 25°C, pH 8.0, mutant enzyme H164W, cosubstrate: 4-estrene-17beta-ol-3-one | Curvularia lunata | |
1.1.1.62 | 0.00028 | - |
NADP+ | 25°C, pH 8.0, mutant enzyme H164G, cosubstrate: 4-estrene-17beta-ol-3-one | Curvularia lunata | |
1.1.1.62 | 0.00035 | - |
NADPH | 25°C, pH 8.0, mutant enzyme H164G, cosubstrate: 4-estrene-3,17-dione | Curvularia lunata | |
1.1.1.62 | 0.0004 | - |
NADPH | 25°C, pH 8.0, mutant enzyme H164W, cosubstrate: 4-estrene-3,17-dione | Curvularia lunata | |
1.1.1.62 | 0.0065 | - |
NADPH | 25°C, pH 8.0, wild-type enzyme, cosubstrate: 4-estrene-3,17-dione | Curvularia lunata | |
1.1.1.62 | 0.16 | - |
NADPH | 25°C, pH 8.0, mutant enzyme Y49D, cosubstrate: 4-estrene-3,17-dione | Curvularia lunata | |
1.1.1.62 | 0.5 | - |
NAD+ | 25°C, pH 8.0, mutant enzyme Y49D, cosubstrate: 4-estrene-17beta-ol-3-one | Curvularia lunata | |
1.1.1.62 | 0.86 | - |
NADH | 25°C, pH 8.0, mutant enzyme Y49D, cosubstrate: 4-estrene-3,17-dione | Curvularia lunata | |
1.1.1.62 | 9 | - |
NADP+ | 25°C, pH 8.0, mutant enzyme Y49D, cosubstrate: 4-estrene-17beta-ol-3-one | Curvularia lunata |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.1.1.62 | Curvularia lunata | O93874 | - |
- |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.1.1.62 | 4-estrene-3,17-dione + NADH + H+ | - |
Curvularia lunata | 4-estrene-17beta-ol-3-one + NAD+ | - |
r | |
1.1.1.62 | 4-estrene-3,17-dione + NADPH + H+ | - |
Curvularia lunata | 4-estrene-17beta-ol-3-one + NADP+ | - |
r | |
1.1.1.62 | 5alpha-androstane-3,17-dione + NADH + H+ | - |
Curvularia lunata | 5alpha-androstane-17beta-ol-3-one + NAD+ | - |
r | |
1.1.1.62 | androst-4-ene-3,17-dione + NADH + H+ | - |
Curvularia lunata | testosterone + NAD+ | - |
r | |
1.1.1.62 | estrone + NADH + H+ | - |
Curvularia lunata | estradiol + NAD+ | - |
r |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.1.1.62 | 17beta-HSDcl | - |
Curvularia lunata |
1.1.1.62 | 17beta-hydroxysteroid dehydrogenase | - |
Curvularia lunata |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.1.1.62 | 0.065 | - |
NADPH | 25°C, pH 8.0, mutant enzyme Y49D, cosubstrate: 4-estrene-3,17-dione | Curvularia lunata | |
1.1.1.62 | 0.24 | - |
NAD+ | 25°C, pH 8.0, mutant enzyme Y49D, cosubstrate: 4-estrene-17beta-ol-3-one | Curvularia lunata | |
1.1.1.62 | 0.27 | - |
NADH | 25°C, pH 8.0, mutant enzyme Y49D, cosubstrate: 4-estrene-3,17-dione | Curvularia lunata | |
1.1.1.62 | 0.54 | - |
NADP+ | 25°C, pH 8.0, mutant enzyme Y49D, cosubstrate: 4-estrene-17beta-ol-3-one | Curvularia lunata | |
1.1.1.62 | 0.65 | - |
NADP+ | 25°C, pH 8.0, wild-type enzyme, cosubstrate: 4-estrene-17beta-ol-3-one | Curvularia lunata | |
1.1.1.62 | 1.8 | - |
NADPH | 25°C, pH 8.0, mutant enzyme H164W, cosubstrate: 4-estrene-3,17-dione | Curvularia lunata | |
1.1.1.62 | 1.9 | - |
NADPH | 25°C, pH 8.0, mutant enzyme H164G, cosubstrate: 4-estrene-3,17-dione | Curvularia lunata | |
1.1.1.62 | 3.1 | - |
NADP+ | 25°C, pH 8.0, mutant enzyme H164W, cosubstrate: 4-estrene-17beta-ol-3-one | Curvularia lunata | |
1.1.1.62 | 3.8 | - |
NADPH | 25°C, pH 8.0, wild-type enzyme, cosubstrate: 4-estrene-3,17-dione | Curvularia lunata | |
1.1.1.62 | 4.7 | - |
NADP+ | 25°C, pH 8.0, mutant enzyme H164G, cosubstrate: 4-estrene-17beta-ol-3-one | Curvularia lunata |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.1.1.62 | NAD+ | wild-type enzyme shows no activity with NADP+ | Curvularia lunata | |
1.1.1.62 | NADH | wild-type enzyme shows no activity with NADPH | Curvularia lunata | |
1.1.1.62 | NADP+ | mutant enzyme Y49D show activity with NADP+, wild-type enzyme shows no activity | Curvularia lunata | |
1.1.1.62 | NADPH | mutant enzyme Y49D show activity with NADPH, wild-type enzyme shows no activity | Curvularia lunata |