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Literature summary extracted from
- Thermostable NAD+-dependent (R)-specific secondary alcohol dehydrogenase from cholesterol-utilizing Burkholderia sp. AIU 652 (2003), J. Biosci. Bioeng., 96, 387-393.
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.1.1.B4 | 8-hydroxyquinoline | 1 mM, 55% residual activity | Burkholderia sp. |  |
| 1.1.1.B4 | alpha,alpha'-dipyridyl | 1 71, 43% residual activity | Burkholderia sp. | |
| 1.1.1.B4 | FeCl2 | 1 mM, 81% residual activity | Burkholderia sp. | |
| 1.1.1.B4 | o-phenanthroline | 1 mM, 43% residual activity | Burkholderia sp. | |
| 1.1.1.B4 | p-chloromercuribenzoate | 1 mM, 4% residual activity | Burkholderia sp. | |
| 1.1.1.B4 | ZnCl2 | 1 mM, 73% residual activity | Burkholderia sp. | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.1.1.B4 | 0.01 | - |
NADH | pH 6.0, 30°C | Burkholderia sp. | |
| 1.1.1.B4 | 0.04 | - |
2-butanone | pH 6.0, 30°C | Burkholderia sp. | |
| 1.1.1.B4 | 0.045 | - |
(R)-2-butanol | pH 8.5, 30°C | Burkholderia sp. | |
| 1.1.1.B4 | 0.065 | - |
2-butanol | pH 8.5, 30°C | Burkholderia sp. | |
| 1.1.1.B4 | 0.065 | - |
acetone | pH 6.0, 30°C | Burkholderia sp. | |
| 1.1.1.B4 | 0.11 | - |
2-propanol | pH 8.5, 30°C | Burkholderia sp. | |
| 1.1.1.B4 | 0.15 | - |
NAD+ | pH 8.5, 30°C | Burkholderia sp. | |
| 1.1.1.B4 | 0.35 | - |
(S)-2-butanol | pH 8.5, 30°C | Burkholderia sp. | |
| 1.1.1.B4 | 0.76 | - |
3-Pentanol | pH 8.5, 30°C | Burkholderia sp. | |
| 1.1.1.B4 | 0.84 | - |
2-Pentanol | pH 8.5, 30°C | Burkholderia sp. | |
| 1.1.1.B4 | 4 | - |
1-propanol | pH 8.5, 30°C | Burkholderia sp. | |
| 1.1.1.B4 | 5.4 | - |
(R)-1,3-butanediol | pH 8.5, 30°C | Burkholderia sp. | |
| 1.1.1.B4 | 6.5 | - |
ethanol | pH 8.5, 30°C | Burkholderia sp. | |
| 1.1.1.B4 | 10 | - |
1-butanol | pH 8.5, 30°C | Burkholderia sp. | |
| 1.1.1.B4 | 52 | - |
(S)-1,3-butanediol | pH 8.5, 30°C | Burkholderia sp. | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 1.1.1.B4 | 37000 | - |
4 * 37000, SDS-PAGE | Burkholderia sp. |
| 1.1.1.B4 | 150000 | - |
gel filtration | Burkholderia sp. |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.1.1.B4 | Burkholderia sp. | - |
growth on cholesterol or propanol as sole carbon source | - |
| 1.1.1.B4 | Burkholderia sp. AIU 652 | - |
growth on cholesterol or propanol as sole carbon source | - |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 1.1.1.B4 | - |
Burkholderia sp. |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 1.1.1.B4 | 104 | - |
pH 8.5, 30°C, substrate 2-propanol | Burkholderia sp. |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.1.1.B4 | (R)-1,3-butanediol + NAD+ | - |
Burkholderia sp. | ? | - |
r | |
| 1.1.1.B4 | (R)-2-butanol + NAD+ | - |
Burkholderia sp. | 2-butanone + NADH + H+ | - |
r | |
| 1.1.1.B4 | (S)-1,3-butanediol + NAD+ | - |
Burkholderia sp. | ? | - |
r | |
| 1.1.1.B4 | 1,2-butanediol + NAD+ | 20% of the activity with 2-propanol | Burkholderia sp. | ? + NADH | - |
r | |
| 1.1.1.B4 | 1,2-propanediol + NAD+ | 45% of the activity with 2-propanol | Burkholderia sp. | ? + NADH | - |
r | |
| 1.1.1.B4 | 1,3-butanediol + NAD+ | 126% of the activity with 2-propanol | Burkholderia sp. | ? + NADH | - |
r | |
| 1.1.1.B4 | 1-butanol + NAD+ | 11% of the activity with 2-propanol | Burkholderia sp. | butanal + NADH | - |
r | |
| 1.1.1.B4 | 1-butanol + NAD+ | 11% of the activity with 2-propanol | Burkholderia sp. AIU 652 | butanal + NADH | - |
r | |
| 1.1.1.B4 | 1-propanol + NAD+ | 9% of the activity with 2-propanol | Burkholderia sp. | propanal + NADH | - |
r | |
| 1.1.1.B4 | 2,3-butanediol + NAD+ | 73% of the activity with 2-propanol | Burkholderia sp. | ? + NADH | - |
r | |
| 1.1.1.B4 | 2,3-butanediol + NAD+ | 73% of the activity with 2-propanol | Burkholderia sp. AIU 652 | ? + NADH | - |
r | |
| 1.1.1.B4 | 2-butanol + NAD+ | (R)-isomer is preferred over (S)-isomer | Burkholderia sp. | 2-butanone + NADH | - |
r | |
| 1.1.1.B4 | 2-butanone + NADH | - |
Burkholderia sp. | (S)-2-hydroxybutane + NAD+ | - |
? | |
| 1.1.1.B4 | 2-butanone + NADH | 83% of the activity with acetone | Burkholderia sp. | 2-butanol + NAD+ | - |
r | |
| 1.1.1.B4 | 2-pentanol + NAD+ | 46% of the activity with 2-propanol | Burkholderia sp. | 2-pentanone + NADH | - |
r | |
| 1.1.1.B4 | 2-pentanol + NAD+ | 46% of the activity with 2-propanol | Burkholderia sp. AIU 652 | 2-pentanone + NADH | - |
r | |
| 1.1.1.B4 | 2-pentanone + NADH + H+ | 44% of the activity with acetone | Burkholderia sp. | 2-pentanol + NAD+ | - |
r | |
| 1.1.1.B4 | 2-propanol + NAD+ | (R)-isomer is preferred over (S)-isomer | Burkholderia sp. | acetone + NADH | - |
r | |
| 1.1.1.B4 | 2-propanol + NAD+ | (R)-isomer is preferred over (S)-isomer | Burkholderia sp. AIU 652 | acetone + NADH | - |
r | |
| 1.1.1.B4 | 3-pentanol + NAD+ | 23% of the activity with 2-propanol | Burkholderia sp. | 3-pentanone + NADH | - |
r | |
| 1.1.1.B4 | 3-pentanone + NADH | 16% of the activity with acetone | Burkholderia sp. | 3-pentanol + NAD+ | - |
r | |
| 1.1.1.B4 | 4-hydroxy-2-butanone + NADH | 95% of the activity with acetone | Burkholderia sp. | ? + NAD+ | - |
r | |
| 1.1.1.B4 | acetone + NADH | - |
Burkholderia sp. | 2-propanol + NAD+ | - |
r | |
| 1.1.1.B4 | ethanol + NAD+ | 4% of the activity with 2-propanol | Burkholderia sp. | ethanal + NADH | - |
r | |
| 1.1.1.B4 | ethanol + NAD+ | 4% of the activity with 2-propanol | Burkholderia sp. AIU 652 | ethanal + NADH | - |
r | |
| 1.1.1.B4 | hydroxyacetone + NADH | 92% of the activity with acetone | Burkholderia sp. | ? + NAD+ | - |
r | |
| 1.1.1.B4 | methylpyruvate + NADH | 64% of the activity with acetone | Burkholderia sp. | ? + NAD+ | - |
r | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 1.1.1.B4 | tetramer | 4 * 37000, SDS-PAGE | Burkholderia sp. |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 1.1.1.B4 | carbonyl reductase (NADH, specific for (S)-configuration of alcohol) | - |
Burkholderia sp. |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.1.1.B4 | 70 | - |
- |
Burkholderia sp. |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.1.1.B4 | 70 | - |
60 min, pH 7.0, 60% residual activity | Burkholderia sp. |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.1.1.B4 | 8 | - |
reduction | Burkholderia sp. |
| 1.1.1.B4 | 8.5 | - |
oxidation, phosphate buffer | Burkholderia sp. |
| 1.1.1.B4 | 9 | - |
and above, oxidation, Tris buffer | Burkholderia sp. |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.1.1.B4 | additional information | no cofactor: NADP+ | Burkholderia sp. | |
| 1.1.1.B4 | NAD+ | - |
Burkholderia sp. | |
| 1.1.1.B4 | NADH | - |
Burkholderia sp. | |
pI Value
| EC Number | Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|---|
| 1.1.1.B4 | Burkholderia sp. | isoelectric focusing | - |
5.4 |
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