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Literature summary extracted from

  • Zheng, Y.; Zhao, L.; Zhang, J.; Zhang, H.; Ma, X.; Wei, D.
    Production of glycerol from glucose by coexpressing glycerol-3-phosphate dehydrogenase and glycerol-3-phosphatase in Klebsiella pneumoniae (2008), J. Biosci. Bioeng., 105, 508-512.
    View publication on PubMed

Application

EC Number Application Comment Organism
1.1.1.8 synthesis successful introduction of a glycerol production pathway into Klebseiella pneumoniae by coexpression of genes encoding glycerol-3-phosphate dehydrogenase and glycerol-3-phosphatase (EC 3.1.3.21) organized into the plasmid pUC18K under control of the respective lac promoter. An engineered Klebsiella pneumoniae that can produce glycerol from glucose is achieved. It is still difficult to efficiently produce 1,3-propanediol from glucose. Only 0.58 g/l 1,3-propanediol is produced Saccharomyces cerevisiae
3.1.3.21 biotechnology production of 1,3-propanediol from glucose in engineered Klebsiella pneumoniae strain Saccharomyces cerevisiae

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.3.21 gpp2 gene from Saccharomyces cerevisiae, transformed into KG1 strain of Klebsiella pneumoniae, puc18K vector Saccharomyces cerevisiae

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.3.21 29000
-
 x * 29000, SDS-PAGE Saccharomyces cerevisiae

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.8 Saccharomyces cerevisiae
-
-
-
1.1.1.8 Saccharomyces cerevisiae Y47
-
-
-
3.1.3.21 Saccharomyces cerevisiae P40106 GPP2; strain Y47 as donor strain for gpp2 gene
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.3.21 SDS-PAGE, recombinant protein Saccharomyces cerevisiae

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.3.21 additional information
-
 changes in glucose, glycerol and 2,3-butanediol determined in engineered bacteria, new metabolic pathway of glucose via glycerol to 1,3-propanediol constructed, effect of pH on production of 1,3-propanediol, effect of dihydroxyacetone and pH, comparison of enzyme activities growing on different substrates, successful introduction of a glycerol pathway of yeast into Klebsiella pneumoniae Saccharomyces cerevisiae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.3.21 glycerol 3-phosphate + H2O enzyme activity of GPP2 of Saccharomyces cerevisiae used for production of glycerol and 1,3-propanediol in engineered Klebsiella pneumonia Saccharomyces cerevisiae glycerol + phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.3.21 ? x * 29000, SDS-PAGE Saccharomyces cerevisiae

Synonyms

EC Number Synonyms Comment Organism
3.1.3.21 Gpp2
-
 Saccharomyces cerevisiae