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Literature summary extracted from

  • Takeo, M.; Nishimura, M.; Takahashi, H.; Kitamura, C.; Kato, D.; Negoro, S.
    Purification and characterization of alkylcatechol 2,3-dioxygenase from butylphenol degradation pathway of Pseudomonas putida MT4 (2007), J. Biosci. Bioeng., 104, 309-314.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.13.11.2 0.0065
-
 4-n-butylcatechol 25°C, pH 7.2 Pseudomonas putida
1.13.11.2 0.0084
-
 4-methylcatechol 25°C, pH 7.2 Pseudomonas putida
1.13.11.2 0.0234
-
 catechol 25°C, pH 7.2 Pseudomonas putida

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.13.11.2 31000
-
 x * 31000, SDS-PAGE Pseudomonas putida

Organism

EC Number Organism UniProt Comment Textmining
1.13.11.2 Pseudomonas putida Q842G7 recombinantly expressed in Escherichia coli
-
1.13.11.2 Pseudomonas putida MT4 Q842G7 recombinantly expressed in Escherichia coli
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.13.11.2
-
 Pseudomonas putida

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.13.11.2 9.7
-
-
 Pseudomonas putida

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.13.11.2 2,3-dihydroxybiphenyl + O2 1.3% of the activity with 2,3-dihydroxybiphenyl Pseudomonas putida ?
-
 ?
1.13.11.2 2,3-dihydroxybiphenyl + O2 1.3% of the activity with 2,3-dihydroxybiphenyl Pseudomonas putida MT4 ?
-
 ?
1.13.11.2 3-methylcatechol + O2 33% of the activity with 4-chlorocatechol Pseudomonas putida ?
-
 ?
1.13.11.2 3-methylcatechol + O2 33% of the activity with 4-chlorocatechol Pseudomonas putida MT4 ?
-
 ?
1.13.11.2 4-chlorocatechol + O2 39% of the activity with 4-chlorocatechol Pseudomonas putida ?
-
 ?
1.13.11.2 4-chlorocatechol + O2 39% of the activity with 4-chlorocatechol Pseudomonas putida MT4 ?
-
 ?
1.13.11.2 4-methylcatechol + O2 activity is 5.72fold higher than with catechol Pseudomonas putida ?
-
 ?
1.13.11.2 4-n-butylcatechol + O2 activity is 1.85fold higher than with catechol Pseudomonas putida ?
-
 ?
1.13.11.2 4-n-heptylcatechol + O2 45% of the activity with catechol Pseudomonas putida ?
-
 ?
1.13.11.2 4-n-hexylcatechol + O2 53% of the activity with catechol Pseudomonas putida ?
-
 ?
1.13.11.2 4-n-nonylcatechol + O2 10% of the activity with catechol Pseudomonas putida ?
-
 ?
1.13.11.2 catechol + O2
-
 Pseudomonas putida 2-hydroxymuconate semialdehyde
-
 ?
1.13.11.2 catechol + O2
-
 Pseudomonas putida MT4 2-hydroxymuconate semialdehyde
-
 ?
1.13.11.2 additional information no activity with 4-tert-butylcatechol Pseudomonas putida ?
-
 ?
1.13.11.2 additional information no activity with 4-tert-butylcatechol Pseudomonas putida MT4 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.13.11.2 ? x * 31000, SDS-PAGE Pseudomonas putida

Synonyms

EC Number Synonyms Comment Organism
1.13.11.2 A23O
-
 Pseudomonas putida
1.13.11.2 BupB
-
 Pseudomonas putida