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Literature summary extracted from

  • Mulyono, R.; Takenaka, S.; Sasano, Y.; Murakami, S.; Aoki, K.
    Bacillus cereus strain 10-L-2 produces two arylamine N-acetyltransferases that transform 4-phenylenediamine into 4-aminoacetanilide (2007), J. Biosci. Bioeng., 103, 147-154.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.3.1.5 Ag+ 26% remaining activity isoenzyme Nat-b; 30% remaining activity isoenzyme NAT-a Bacillus cereus
2.3.1.5 Co2+ 39% remaining activity isoenzyme NAT-a Bacillus cereus
2.3.1.5 Cu2+ 30% remaining activity isoenzyme Nat-b Bacillus cereus
2.3.1.5 Hg2+ 11% remaining activity isoenzyme Nat-b; 36% remaining activity isoenzyme NAT-a Bacillus cereus
2.3.1.5 iodoacetic acid 13% remaining activity Bacillus cereus
2.3.1.5 Zn2+ 39% remaining activity isoenzyme Nat-b; 41% remaining activity isoenzyme NAT-a Bacillus cereus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.1.5 3.2
-
 4-phenylenediamine
-
 Bacillus cereus
2.3.1.5 3.2
-
 N,N-1,4-phenylenediamine Vmax: 0.17 micromol/min/mg Bacillus cereus
2.3.1.5 5.4
-
 4-phenylenediamine
-
 Bacillus cereus
2.3.1.5 5.4
-
 N,N-1,4-phenylenediamine Vmax: 2.1 micromol/min/mg Bacillus cereus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.3.1.5 27500
-
 NAT-b, determined by SDS-PAGE Bacillus cereus
2.3.1.5 27500
-
 SDS-PAGE, isoenzyme Nat-b Bacillus cereus
2.3.1.5 31000
-
 NAT-a, determined by SDS-PAGE Bacillus cereus
2.3.1.5 31000
-
 SDS-PAGE, isoenzyme NAT-a Bacillus cereus
2.3.1.5 35000
-
 gel filtration, isoenzyme NAT-a Bacillus cereus
2.3.1.5 35000
-
 gel filtration, isoenzyme Nat-b Bacillus cereus
2.3.1.5 35000
-
 NAT-a, determined by gel filtration Bacillus cereus
2.3.1.5 35000
-
 NAT-b, determined by gel filtration Bacillus cereus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.5 acetyl-CoA + an N-hydroxyarylamine Bacillus cereus
-
 CoA + an N-acetoxyarylamine
-
 ?
2.3.1.5 acetyl-CoA + an N-hydroxyarylamine Bacillus cereus 10-L-2
-
 CoA + an N-acetoxyarylamine
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.5 Bacillus cereus
-
-
-
2.3.1.5 Bacillus cereus 10-L-2
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.1.5 NAT is purified from the extract of Bacillus cereus cultures using ammonium sulfate precipitation, a DEAE-cellulose, a DEAE-Toyopearl and a hydrophobic POROS 20PE column Bacillus cereus
2.3.1.5 using DE52 column chromatography, DEAE-Toypearl columns and hydrophobic POROS 20PE columns Bacillus cereus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.3.1.5 additional information
-
 relative activity isoenzyme NAT-a (substrate): 12% (aniline), 90% (2-phenylendiamine), 0% (3-phenylendiamine), 100% (4-phenylendiamine), 72% (2-aminophenol), 33% (3-aminophenol), 94% (4-aminophenol), 0% (2-aminobenzoic acid), 122% (3-aminobenzoic acid), 19% (4-aminobenzoic acid), 0% (2-nitroaniline), 2% (3-nitroaniline), 0% (4-nitroaniline), 18% (2-chloraniline), 48% (3-chloraniline), 18% (4-chloraniline), 0% (2-toluidine), 21% (3-toluidine), 80% (4-toluidine), 46% (sulfamethazine), 90% (4-aminosalicylic acid), 550% (5-aminosalicylic acid), 0% (4-aminoacetanilide) Bacillus cereus
2.3.1.5 additional information
-
 relative activity isoenzyme Nat-b (substrate): 24% (aniline), 80% (2-phenylendiamine), 0% (3-phenylendiamine), 100% (4-phenylendiamine), 36% (2-aminophenol), 56% (3-aminophenol), 48% (4-aminophenol), 0% (2-aminobenzoic acid), 105% (3-aminobenzoic acid), 26% (4-aminobenzoic acid), 3% (2-nitroaniline), 6% (3-nitroaniline), 0% (4-nitroaniline), 12% (2-chloraniline), 48% (3-chloraniline), 21% (4-chloraniline), 0% (2-toluidine), 28% (3-toluidine), 40% (4-toluidine), 0% (sulfamethazine), 100% (4-aminosalicylic acid), 510% (5-aminosalicylic acid), 0% (4-aminoacetanilide) Bacillus cereus
2.3.1.5 0.19
-
 after streptomycin sulfate treatment Bacillus cereus
2.3.1.5 0.25
-
 cell extract Bacillus cereus
2.3.1.5 0.38
-
 purification step ammonium sulfate precipitation Bacillus cereus
2.3.1.5 7
-
 purification step POROS 20PE column Bacillus cereus
2.3.1.5 7.3
-
 purification step DE52-cellulose column Bacillus cereus
2.3.1.5 9
-
 purification step DE52-cellulose column Bacillus cereus
2.3.1.5 9
-
 purification step POROS 20PE column Bacillus cereus
2.3.1.5 26
-
 purification step DEAE Toyopearl column Bacillus cereus
2.3.1.5 27
-
 purification step DEAE Toyopearl column Bacillus cereus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.5 acetyl-CoA + 2-aminobenzoic acid relative activity: 0% Bacillus cereus CoA + N-acetyl-2-aminobenzoic acid
-
 ?
2.3.1.5 acetyl-CoA + 2-aminophenol relative activity: 36% Bacillus cereus CoA + N-acetyl-2-aminophenol
-
 ?
2.3.1.5 acetyl-CoA + 2-aminophenol relative activity: 72% Bacillus cereus CoA + N-acetyl-2-aminophenol
-
 ?
2.3.1.5 acetyl-CoA + 2-chloroaniline relative activity: 12% Bacillus cereus CoA + N-acetyl-2-chloroaniline
-
 ?
2.3.1.5 acetyl-CoA + 2-chloroaniline relative activity: 18% Bacillus cereus CoA + N-acetyl-2-chloroaniline
-
 ?
2.3.1.5 acetyl-CoA + 2-nitroaniline relative activity: 0% Bacillus cereus CoA + N-acetyl-2-nitroaniline
-
 ?
2.3.1.5 acetyl-CoA + 2-nitroaniline relative activity: 3% Bacillus cereus CoA + N-acetyl-2-nitroaniline
-
 ?
2.3.1.5 acetyl-CoA + 2-phenylendiamine relative activity: 80% Bacillus cereus CoA + ?
-
 ?
2.3.1.5 acetyl-CoA + 2-phenylendiamine relative activity: 90% Bacillus cereus CoA + ?
-
 ?
2.3.1.5 acetyl-CoA + 2-toluidine relative activity: 0% Bacillus cereus CoA + N-acetyl-2-toluidine
-
 ?
2.3.1.5 acetyl-CoA + 3-aminobenzoic acid relative activity: 105% Bacillus cereus CoA + N-acetyl-3-aminobenzoic acid
-
 ?
2.3.1.5 acetyl-CoA + 3-aminobenzoic acid relative activity: 122% Bacillus cereus CoA + N-acetyl-3-aminobenzoic acid
-
 ?
2.3.1.5 acetyl-CoA + 3-aminobenzoic acid relative activity: 105% Bacillus cereus 10-L-2 CoA + N-acetyl-3-aminobenzoic acid
-
 ?
2.3.1.5 acetyl-CoA + 3-aminobenzoic acid relative activity: 122% Bacillus cereus 10-L-2 CoA + N-acetyl-3-aminobenzoic acid
-
 ?
2.3.1.5 acetyl-CoA + 3-aminophenol relative activity: 33% Bacillus cereus CoA + N-acetyl-3-aminophenol
-
 ?
2.3.1.5 acetyl-CoA + 3-aminophenol relative activity: 56% Bacillus cereus CoA + N-acetyl-3-aminophenol
-
 ?
2.3.1.5 acetyl-CoA + 3-chloraniline relative activity: 48% Bacillus cereus CoA + N-acetyl-3-chloroaniline
-
 ?
2.3.1.5 acetyl-CoA + 3-nitroaniline relative activity: 2% Bacillus cereus CoA + N-acetyl-3-nitroaniline
-
 ?
2.3.1.5 acetyl-CoA + 3-nitroaniline relative activity: 6% Bacillus cereus CoA + N-acetyl-3-nitroaniline
-
 ?
2.3.1.5 acetyl-CoA + 3-phenylendiamine relative activity: 0% Bacillus cereus CoA + ?
-
 ?
2.3.1.5 acetyl-CoA + 3-toluidine relative activity: 21% Bacillus cereus CoA + N-acetyl-3-toluidine
-
 ?
2.3.1.5 acetyl-CoA + 3-toluidine relative activity: 28% Bacillus cereus CoA + N-acetyl-3-toluidine
-
 ?
2.3.1.5 acetyl-CoA + 4-aminoacetanilide relative activity: 0% Bacillus cereus CoA + N-acetyl-4-aminoacetanilide
-
 ?
2.3.1.5 acetyl-CoA + 4-aminobenzoic acid relative activity: 19% Bacillus cereus CoA + N-acetyl-4-aminobenzoic acid
-
 ?
2.3.1.5 acetyl-CoA + 4-aminobenzoic acid relative activity: 26% Bacillus cereus CoA + N-acetyl-4-aminobenzoic acid
-
 ?
2.3.1.5 acetyl-CoA + 4-aminophenol relative activity: 48% Bacillus cereus CoA + N-acetyl-4-aminophenol
-
 ?
2.3.1.5 acetyl-CoA + 4-aminophenol relative activity: 94% Bacillus cereus CoA + N-acetyl-4-aminophenol
-
 ?
2.3.1.5 acetyl-CoA + 4-aminosalicylic acid relative activity: 100% Bacillus cereus CoA + N-acetyl-4-aminosalicylic acid
-
 ?
2.3.1.5 acetyl-CoA + 4-aminosalicylic acid relative activity: 90% Bacillus cereus CoA + N-acetyl-4-aminosalicylic acid
-
 ?
2.3.1.5 acetyl-CoA + 4-chloroaniline relative activity: 18% Bacillus cereus CoA + N-acetyl-4-chloroaniline
-
 ?
2.3.1.5 acetyl-CoA + 4-chloroaniline relative activity: 21% Bacillus cereus CoA + N-acetyl-4-chloroaniline
-
 ?
2.3.1.5 acetyl-CoA + 4-nitroaniline relative activity: 0% Bacillus cereus CoA + N-acetyl-4-nitroaniline
-
 ?
2.3.1.5 acetyl-CoA + 4-nitroaniline relative activity: 0% Bacillus cereus 10-L-2 CoA + N-acetyl-4-nitroaniline
-
 ?
2.3.1.5 acetyl-CoA + 4-phenylenediamine
-
 Bacillus cereus CoA + 4-aminoacetanilide
-
 ?
2.3.1.5 acetyl-CoA + 4-toluidine relative activity: 40% Bacillus cereus CoA + N-acetyl-4-toluidine
-
 ?
2.3.1.5 acetyl-CoA + 4-toluidine relative activity: 80% Bacillus cereus CoA + N-acetyl-4-toluidine
-
 ?
2.3.1.5 acetyl-CoA + 5-aminosalicylic acid
-
 Bacillus cereus CoA + N-acetyl-5-aminosalicylic acid
-
 ?
2.3.1.5 acetyl-CoA + 5-aminosalicylic acid relative activity: 510% Bacillus cereus CoA + N-acetyl-5-aminosalicylic acid
-
 ?
2.3.1.5 acetyl-CoA + 5-aminosalicylic acid relative activity: 550% Bacillus cereus CoA + N-acetyl-5-aminosalicylic acid
-
 ?
2.3.1.5 acetyl-CoA + an N-hydroxyarylamine
-
 Bacillus cereus CoA + an N-acetoxyarylamine
-
 ?
2.3.1.5 acetyl-CoA + an N-hydroxyarylamine
-
 Bacillus cereus 10-L-2 CoA + an N-acetoxyarylamine
-
 ?
2.3.1.5 acetyl-CoA + aniline relative activity: 12% Bacillus cereus CoA + N-acetyl-aniline
-
 ?
2.3.1.5 acetyl-CoA + aniline relative activity: 24% Bacillus cereus CoA + N-acetyl-aniline
-
 ?
2.3.1.5 acetyl-CoA + N,N-1,4-phenylenediamine relative activity: 100% Bacillus cereus CoA + ?
-
 ?
2.3.1.5 acetyl-CoA + sulfamethazine
-
 Bacillus cereus CoA + N-4-acetylsulfamethazine
-
 ?
2.3.1.5 acetyl-CoA + sulfamethazine
-
 Bacillus cereus 10-L-2 CoA + N-4-acetylsulfamethazine
-
 ?
2.3.1.5 acetyl-CoA + sulfamethazine relative activity: 0% Bacillus cereus CoA + N-acetyl-sulfamethazine
-
 ?
2.3.1.5 acetyl-CoA + sulfamethazine relative activity: 46% Bacillus cereus CoA + N-acetyl-sulfamethazine
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.3.1.5 arylamine N-acetyltransferase
-
 Bacillus cereus
2.3.1.5 N-acetyltransferase a
-
 Bacillus cereus
2.3.1.5 N-acetyltransferase b
-
 Bacillus cereus
2.3.1.5 NAT-a
-
 Bacillus cereus
2.3.1.5 NAT-b
-
 Bacillus cereus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.3.1.5 30
-
 assay at Bacillus cereus
2.3.1.5 30
-
 activity assay Bacillus cereus
2.3.1.5 37
-
 enzyme assayed at various temperatures Bacillus cereus
2.3.1.5 37
-
 optimum temperature Bacillus cereus

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
2.3.1.5 42
-
 30% activity Bacillus cereus
2.3.1.5 42
-
 50% activity Bacillus cereus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.3.1.5 additional information
-
 incubation at 42°C for 10 min: 30% remaining activity isoenzyme NAT-a Bacillus cereus
2.3.1.5 additional information
-
 incubation at 42°C for 10 min: 50% remaining activity isoenzyme Nat-b Bacillus cereus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.3.1.5 7.1
-
 assay at Bacillus cereus
2.3.1.5 7.1
-
 activity assay Bacillus cereus
2.3.1.5 7.5
-
 pH-optimum Bacillus cereus
2.3.1.5 7.5
-
 enzyme assayed at various pHs at 30°C Bacillus cereus
2.3.1.5 8
-
 pH-optimum Bacillus cereus
2.3.1.5 8
-
 enzyme assayed at various pHs at 30°C Bacillus cereus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.3.1.5 6.5 9.5 80% activity Bacillus cereus
2.3.1.5 7 9.5 80% activity Bacillus cereus

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
2.3.1.5 6.5 9.5 incubation overnight at pH 6.5-9.5: 80% remaining activity isoenzyme Nat-b Bacillus cereus
2.3.1.5 7 9.5 incubation overnight at pH 7-9.5: 80% remaining activity isoenzyme NAT-a Bacillus cereus

Cofactor

EC Number Cofactor Comment Organism Structure
2.3.1.5 acetyl-CoA
-
 Bacillus cereus