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Literature summary extracted from
- Molecular cloning and characterization of gene encoding novel puromycin-inactivating enzyme from blasticidin S-producing Streptomyces morookaensis (2006), J. Biosci. Bioeng., 101, 63-69.
Activating Compound
| EC Number | Activating Compound | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.4.19.1 | dithiothreitol | maintains catalytic activity of PMH | Streptomyces morookaense |  |
Application
| EC Number | Application | Comment | Organism |
|---|---|---|---|
| 3.4.19.1 | additional information | amino acid sequence of PMH displays high similarity to that of the Streptomyces acyl-peptide hydrolase. PMH is an aminopeptidase carrying a putative catalytic triad, Ser511-Asp593-His625, with broad substrate specificity | Streptomyces morookaense |
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 3.4.19.1 | pTrc-PMH overexpressed in Escherichia coli strain JM105 | Streptomyces morookaense |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.4.19.1 | 0.013 | - |
Pro-beta-naphthylamide | - |
Streptomyces morookaense | |
| 3.4.19.1 | 0.028 | - |
Phe-beta-naphthylamide | - |
Streptomyces morookaense | |
| 3.4.19.1 | 0.067 | - |
Tyr-beta-naphthylamide | - |
Streptomyces morookaense | |
| 3.4.19.1 | 0.12 | - |
Leu-beta-naphthylamide | - |
Streptomyces morookaense | |
| 3.4.19.1 | 0.39 | - |
Ala-beta-naphthylamide | - |
Streptomyces morookaense | |
| 3.4.19.1 | 1.91 | - |
puromycin | - |
Streptomyces morookaense | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.4.19.1 | Streptomyces morookaense | - |
strain JCM4673 | - |
| 3.4.19.1 | Streptomyces morookaense JCM 4673 | - |
strain JCM4673 | - |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.4.19.1 | by gel filtration, to homogeneity | Streptomyces morookaense |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 3.4.19.1 | 0.124 | - |
recombinant enzyme, after the induction with IPTG, at 8 h of culture in LB medium | Streptomyces morookaense |
| 3.4.19.1 | 0.172 | - |
recombinant enzyme, after the induction with IPTG, at 18 h of culture in TB medium | Streptomyces morookaense |
| 3.4.19.1 | 13 | - |
with Tyr-Leu as substrate | Streptomyces morookaense |
| 3.4.19.1 | 19 | - |
with Tyr-Phe as substrate | Streptomyces morookaense |
| 3.4.19.1 | 23 | - |
with Ala-p-nitroanilide as substrate | Streptomyces morookaense |
| 3.4.19.1 | 154 | - |
with Phe-p-nitroanilide as substrate | Streptomyces morookaense |
| 3.4.19.1 | 186 | - |
with Ala-beta-naphthylamide as substrate | Streptomyces morookaense |
Storage Stability
| EC Number | Storage Stability | Organism |
|---|---|---|
| 3.4.19.1 | -30°C, 20% glycerol | Streptomyces morookaense |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.4.19.1 | Ala-beta-naphthylamide + H2O | - |
Streptomyces morookaense | Ala + 2-naphthylamine | - |
? | |
| 3.4.19.1 | Ala-beta-naphthylamide + H2O | - |
Streptomyces morookaense JCM 4673 | Ala + 2-naphthylamine | - |
? | |
| 3.4.19.1 | Ala-p-nitroanilide + H2O | prefered substrate for PMH | Streptomyces morookaense | Ala + p-nitroaniline | - |
? | |
| 3.4.19.1 | Ala-p-nitroanilide + H2O | prefered substrate for PMH | Streptomyces morookaense JCM 4673 | Ala + p-nitroaniline | - |
? | |
| 3.4.19.1 | Leu-beta-naphthylamide + H2O | - |
Streptomyces morookaense | Leu + 2-naphthylamine | - |
? | |
| 3.4.19.1 | Leu-beta-naphthylamide + H2O | - |
Streptomyces morookaense JCM 4673 | Leu + 2-naphthylamine | - |
? | |
| 3.4.19.1 | Phe-beta-naphthylamide + H2O | - |
Streptomyces morookaense | Phe + 2-naphthylamine | - |
? | |
| 3.4.19.1 | Phe-p-nitroanilide + H2O | prefered substrate for PMH | Streptomyces morookaense | Phe + p-nitroaniline | - |
? | |
| 3.4.19.1 | Pro-beta-naphthylamide + H2O | - |
Streptomyces morookaense | Pro + 2-naphthylamine | - |
? | |
| 3.4.19.1 | puromycin + H2O | - |
Streptomyces morookaense | ? | - |
? | |
| 3.4.19.1 | Tyr-beta-naphthylamide + H2O | - |
Streptomyces morookaense | Tyr + 2-naphthylamine | - |
? | |
| 3.4.19.1 | Tyr-Leu + H2O | - |
Streptomyces morookaense | Tyr + Leu | - |
? | |
| 3.4.19.1 | Tyr-Leu + H2O | - |
Streptomyces morookaense JCM 4673 | Tyr + Leu | - |
? | |
| 3.4.19.1 | Tyr-Phe + H2O | - |
Streptomyces morookaense | Tyr + Phe | - |
? | |
| 3.4.19.1 | Tyr-Phe + H2O | - |
Streptomyces morookaense JCM 4673 | Tyr + Phe | - |
? | |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 3.4.19.1 | PM hydrolase | - |
Streptomyces morookaense |
| 3.4.19.1 | PMH | - |
Streptomyces morookaense |
Turnover Number [1/s]
| EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.4.19.1 | 0.98 | - |
puromycin | - |
Streptomyces morookaense | |
| 3.4.19.1 | 1.61 | - |
Pro-beta-naphthylamide | - |
Streptomyces morookaense | |
| 3.4.19.1 | 1.69 | - |
Phe-beta-naphthylamide | - |
Streptomyces morookaense | |
| 3.4.19.1 | 1.85 | - |
Tyr-beta-naphthylamide | - |
Streptomyces morookaense | |
| 3.4.19.1 | 4.07 | - |
Leu-beta-naphthylamide | - |
Streptomyces morookaense | |
| 3.4.19.1 | 8.79 | - |
Ala-beta-naphthylamide | - |
Streptomyces morookaense | |
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