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Literature summary extracted from

  • Ghosh, A.K.; Ramakrishnan, G.; Rajasekharan, R.
    YLR099C (ICT1) encodes a soluble Acyl-CoA-dependent lysophosphatidic acid acyltransferase responsible for enhanced phospholipid synthesis on organic solvent stress in Saccharomyces cerevisiae (2008), J. Biol. Chem., 283, 9768-9775.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.1.51 expressed in Escherichia coli, overexpression in Saccharomyces cerevisiae, expression plasmid pPS189 Saccharomyces cerevisiae

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.51 acyl-CoA + 1-acyl-sn-glycerol 3-phosphate Saccharomyces cerevisiae
-
 CoA + 1,2-diacyl-sn-glycerol 3-phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.51 Saccharomyces cerevisiae
-
 BY4741 and deletion mutant strain of ICT1 protein in BY4741-background
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.1.51 gel filtration and SDS-PAGE, recombinant protein Saccharomyces cerevisiae

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.3.1.51 cell culture
-
 Saccharomyces cerevisiae
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.3.1.51 additional information
-
 absence of transmembrane domains, presence of hydrolase/acyltransferase domain with a distinct lipid-binding motif and a lysophospholipase domain, reduced phosphatidic acid in mutants suggests a role in phosphatidic acid biosynthesis, overexpression shows increase in phosphatidic acid and other phospholipids on organic solvent exposure, recombinant protein acylates lysophosphatidic acid, substrate preference analyzed, nearly no activity with lysophospholipids such as lysophosphatidylcholine (LPC), lysophosphatidylethanolamine (LPE), and lysophosphatidylserine (LPS) Saccharomyces cerevisiae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.51 acyl-CoA + 1-acyl-sn-glycerol 3-phosphate
-
 Saccharomyces cerevisiae CoA + 1,2-diacyl-sn-glycerol 3-phosphate
-
 ?
2.3.1.51 oleoyl-CoA + 1-oleoyl-sn-glycerol-3-phosphate substrate preference higher for oleoyl-CoA than for palmitoyl-CoA and stearoyl-CoA Saccharomyces cerevisiae CoA + 1,2-dioleoyl-sn-glycerol 3-phosphate
-
 ?
2.3.1.51 palmitoyl-CoA + 1-acyl-sn-glycerol 3-phosphate substrate preference lower than for oleoyl-CoA Saccharomyces cerevisiae CoA + 1-acyl-2-palmitoyl-sn-glycerol 3-phosphate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.3.1.51 ICT1 protein
-
 Saccharomyces cerevisiae
2.3.1.51 lysophosphatidic acid ayltransferase
-
 Saccharomyces cerevisiae
2.3.1.51 oleoyl-CoA lysophosphatidic acid acyltransferase
-
 Saccharomyces cerevisiae

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.3.1.51 30
-
 activity assay at Saccharomyces cerevisiae