BRENDA - Enzyme Database

Betaine-homocysteine S-methyltransferase-2 is an S-methylmethionine-homocysteine methyltransferase

Szegedi, S.S.; Castro, C.C.; Koutmos, M.; Garrow, T.A.; J. Biol. Chem. 283, 8939-8945 (2008)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Commentary
Organism
2.1.1.10
expressed in Escherichia coli, recombinant protein, intein/chitin-binding fusion constructs, pTBY3 vector
Homo sapiens
General Stability
EC Number
General Stability
Organism
2.1.1.10
high concentrations of glycerol (40%, v/v) greatly stabilized activity
Homo sapiens
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
2.1.1.5
AdoMet
weak inhibitor, at 2 mM: 24% inhibition
Homo sapiens
2.1.1.5
AdoMet
weak inhibitor, at 2.5 mM: 15% inhibition
Mus musculus
2.1.1.5
dimethylglycine
at 0.2 mM: 80% inhibition, at 2 mM: 97% inhibition; weak inhibitor, at 2 mM: 11% inhibition
Homo sapiens
2.1.1.5
dimethylglycine
at 0.25 mM: 64% inhibition, at 2.5 mM: 95% inhibition; weak inhibitor, at 2.5 mM: 19% inhibition
Mus musculus
2.1.1.5
dimethylsulfonioacetate
at 2 mM: 79% inhibition; weak inhibitor, at 2 mM: 20% inhibition
Homo sapiens
2.1.1.5
dimethylsulfonioacetate
at 0.25 mM: 29% inhibition, at 2.5 mM: 82% inhibition
Mus musculus
2.1.1.5
dimethylsulfoniopropionate
weak inhibitor, at 2 mM: 29% inhibition
Homo sapiens
2.1.1.5
dimethylsulfoniopropionate
weak inhibitor, at 2.5 mM: 11% inhibition; weak inhibitor, at 2.5 mM: 17% inhibition
Mus musculus
2.1.1.5
methionine
at 2 mM: 60% inhibition; weak inhibitor at 2 mM: 15% inhibition
Homo sapiens
2.1.1.5
methionine
at 2.5 mM: 38% inhibition; at 2.5 mM: 48% inhibition
Mus musculus
2.1.1.5
S-(delta-carboxybutyl)-L-homocysteine
at 0.05 mM: 97% inhibition, at 0.5 mM: total inhibition; weak inhibitor, at 0.5 mM: 26% inhibition
Homo sapiens
2.1.1.5
S-(delta-carboxybutyl)-L-homocysteine
at 0.025 mM: 22% inhibition, at 0.0625 mM: 36% inhibition, at 0.125 mM: 49% inhibition, at 0.5 mM: 81% inhibition; total inhibition
Mus musculus
2.1.1.10
L-methionine
stronger inhibitor of BHMT-2 than of BHMT (EC 2.1.1.5)
Homo sapiens
2.1.1.10
additional information
relative inhibition of human BHMT (EC 2.1.1.5) and human BHMT-2 activities by methyl donor substrates determined
Homo sapiens
2.1.1.10
S-adenosyl-L-methionine
weak inhibitor of, does not inhibit BHMT (EC 2.1.1.5), not inhibited by betaine
Homo sapiens
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.1.1.5
0.76
-
S-methyl-L-methionine
-
Mus musculus
2.1.1.5
0.94
-
S-methyl-L-methionine
-
Homo sapiens
2.1.1.5
2.2
-
betaine
-
Homo sapiens
2.1.1.5
3
-
S-methyl-L-methionine
-
Homo sapiens
2.1.1.10
0.94
-
S-methyl-L-methionine
final concentrations of S-methyl-L-methionine ranging from 0.1 mM to 9 mM, lower value than obtained for BHMT (EC 2.1.1.5)
Homo sapiens
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
2.1.1.5
Zn
BHMT-2 is a zinc metalloenzyme
Homo sapiens
2.1.1.5
Zn
BHMT-2 is a zinc metalloenzyme
Mus musculus
2.1.1.5
Zn2+
BHMT is a zinc metalloenzyme
Homo sapiens
2.1.1.5
Zn2+
BHMT-2 is a zinc metalloenzyme
Mus musculus
2.1.1.10
Zn2+
required for activity
Homo sapiens
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.1.1.10
40000
-
SDS-PAGE, 73% sequence identity with the 45 kDa BHMT protein (EC 2.1.1.5)
Homo sapiens
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.1.1.5
Homo sapiens
-
-
-
2.1.1.5
Mus musculus
-
-
-
2.1.1.10
Homo sapiens
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
2.1.1.10
gel filtration, recombinant protein
Homo sapiens
Reaction
EC Number
Reaction
Commentary
Organism
2.1.1.10
S-methyl-L-methionine + L-homocysteine = 2 L-methionine
high homology to betaine-homocysteine methyltransferase (BHMT, EC 2.1.1.5), betaine is not a substrate for BHMT-2 but high activity with S-methyl-L-methionine
Homo sapiens
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
2.1.1.5
kidney
-
Mus musculus
-
2.1.1.5
liver
-
Mus musculus
-
2.1.1.10
kidney
tissue extracts, activities measured in
Homo sapiens
-
2.1.1.10
liver
tissue extracts, activities measured in
Homo sapiens
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
2.1.1.10
additional information
-
structural comparison and enzymatic properties of purified human betaine-homocysteine methyltransferase (BHMT, EC 2.1.1.5) and human betaine-homocysteine methyltransferase-2 (BHMT-2) determined, methylation capacities of homocysteine analyzed, S-adenosyl-L-methionine-dependent methylation of homocysteine predicted mostly occurs via BHMT-2 in vivo
Homo sapiens
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.1.1.5
L-homocysteine + betaine
-
687808
Mus musculus
L-methionine + dimethylglycine
-
-
-
?
2.1.1.5
L-homocysteine + betaine
-
687808
Homo sapiens
L-methionine + dimethylglycine
-
-
-
?
2.1.1.5
L-homocysteine + S-methyl-L-methionine
-
687808
Mus musculus
?
-
-
-
?
2.1.1.5
L-homocysteine + S-methyl-L-methionine
-
687808
Homo sapiens
?
-
-
-
?
2.1.1.5
L-homocysteine + S-methyl-L-methionine
BHMT-2 uses S-methylmethionine as a methyl donor for the methylation of homocysteine. Unlike BHMT, BHMT-2 can not use betaine
687808
Homo sapiens
?
-
-
-
?
2.1.1.5
L-homocysteine + S-methylmethionine
BHMT-2 uses S-methylmethionine as a methyl donor for the methylation of homocysteine. Unlike BHMT, BHMT-2 can not use betaine
687808
Mus musculus
?
-
-
-
?
2.1.1.10
S-adenosyl-L-methionine + L-homocysteine
low activity
687808
Homo sapiens
?
-
-
-
?
2.1.1.10
S-methyl-L-methionine + L-homocysteine
high homology to betaine-homocysteine methyltransferase (BHMT, EC 2.1.1.5), betaine is not a substrate for BHMT-2 but high activity with S-methyl-L-methionine
687808
Homo sapiens
?
-
-
-
?
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
2.1.1.10
7.5
-
activity assay at
Homo sapiens
Turnover Number [1/s]
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
2.1.1.5
24
-
S-methyl-L-methionine
-
Homo sapiens
2.1.1.5
38
-
S-methyl-L-methionine
-
Homo sapiens
2.1.1.5
88
-
betaine
-
Homo sapiens
2.1.1.10
additional information
-
additional information
similar turnover number like betaine-homocysteine methyltransferase (BHMT, EC 2.1.1.5)
Homo sapiens
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.1.1.5
7.5
-
assay at
Mus musculus
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
2.1.1.10
expressed in Escherichia coli, recombinant protein, intein/chitin-binding fusion constructs, pTBY3 vector
Homo sapiens
General Stability (protein specific)
EC Number
General Stability
Organism
2.1.1.10
high concentrations of glycerol (40%, v/v) greatly stabilized activity
Homo sapiens
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
2.1.1.5
AdoMet
weak inhibitor, at 2 mM: 24% inhibition
Homo sapiens
2.1.1.5
AdoMet
weak inhibitor, at 2.5 mM: 15% inhibition
Mus musculus
2.1.1.5
dimethylglycine
at 0.2 mM: 80% inhibition, at 2 mM: 97% inhibition; weak inhibitor, at 2 mM: 11% inhibition
Homo sapiens
2.1.1.5
dimethylglycine
at 0.25 mM: 64% inhibition, at 2.5 mM: 95% inhibition; weak inhibitor, at 2.5 mM: 19% inhibition
Mus musculus
2.1.1.5
dimethylsulfonioacetate
at 2 mM: 79% inhibition; weak inhibitor, at 2 mM: 20% inhibition
Homo sapiens
2.1.1.5
dimethylsulfonioacetate
at 0.25 mM: 29% inhibition, at 2.5 mM: 82% inhibition
Mus musculus
2.1.1.5
dimethylsulfoniopropionate
weak inhibitor, at 2 mM: 29% inhibition
Homo sapiens
2.1.1.5
dimethylsulfoniopropionate
weak inhibitor, at 2.5 mM: 11% inhibition; weak inhibitor, at 2.5 mM: 17% inhibition
Mus musculus
2.1.1.5
methionine
at 2 mM: 60% inhibition; weak inhibitor at 2 mM: 15% inhibition
Homo sapiens
2.1.1.5
methionine
at 2.5 mM: 38% inhibition; at 2.5 mM: 48% inhibition
Mus musculus
2.1.1.5
S-(delta-carboxybutyl)-L-homocysteine
at 0.05 mM: 97% inhibition, at 0.5 mM: total inhibition; weak inhibitor, at 0.5 mM: 26% inhibition
Homo sapiens
2.1.1.5
S-(delta-carboxybutyl)-L-homocysteine
at 0.025 mM: 22% inhibition, at 0.0625 mM: 36% inhibition, at 0.125 mM: 49% inhibition, at 0.5 mM: 81% inhibition; total inhibition
Mus musculus
2.1.1.10
L-methionine
stronger inhibitor of BHMT-2 than of BHMT (EC 2.1.1.5)
Homo sapiens
2.1.1.10
additional information
relative inhibition of human BHMT (EC 2.1.1.5) and human BHMT-2 activities by methyl donor substrates determined
Homo sapiens
2.1.1.10
S-adenosyl-L-methionine
weak inhibitor of, does not inhibit BHMT (EC 2.1.1.5), not inhibited by betaine
Homo sapiens
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.1.1.5
0.76
-
S-methyl-L-methionine
-
Mus musculus
2.1.1.5
0.94
-
S-methyl-L-methionine
-
Homo sapiens
2.1.1.5
2.2
-
betaine
-
Homo sapiens
2.1.1.5
3
-
S-methyl-L-methionine
-
Homo sapiens
2.1.1.10
0.94
-
S-methyl-L-methionine
final concentrations of S-methyl-L-methionine ranging from 0.1 mM to 9 mM, lower value than obtained for BHMT (EC 2.1.1.5)
Homo sapiens
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
2.1.1.5
Zn
BHMT-2 is a zinc metalloenzyme
Homo sapiens
2.1.1.5
Zn
BHMT-2 is a zinc metalloenzyme
Mus musculus
2.1.1.5
Zn2+
BHMT is a zinc metalloenzyme
Homo sapiens
2.1.1.5
Zn2+
BHMT-2 is a zinc metalloenzyme
Mus musculus
2.1.1.10
Zn2+
required for activity
Homo sapiens
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.1.1.10
40000
-
SDS-PAGE, 73% sequence identity with the 45 kDa BHMT protein (EC 2.1.1.5)
Homo sapiens
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
2.1.1.10
gel filtration, recombinant protein
Homo sapiens
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
2.1.1.5
kidney
-
Mus musculus
-
2.1.1.5
liver
-
Mus musculus
-
2.1.1.10
kidney
tissue extracts, activities measured in
Homo sapiens
-
2.1.1.10
liver
tissue extracts, activities measured in
Homo sapiens
-
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
2.1.1.10
additional information
-
structural comparison and enzymatic properties of purified human betaine-homocysteine methyltransferase (BHMT, EC 2.1.1.5) and human betaine-homocysteine methyltransferase-2 (BHMT-2) determined, methylation capacities of homocysteine analyzed, S-adenosyl-L-methionine-dependent methylation of homocysteine predicted mostly occurs via BHMT-2 in vivo
Homo sapiens
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.1.1.5
L-homocysteine + betaine
-
687808
Mus musculus
L-methionine + dimethylglycine
-
-
-
?
2.1.1.5
L-homocysteine + betaine
-
687808
Homo sapiens
L-methionine + dimethylglycine
-
-
-
?
2.1.1.5
L-homocysteine + S-methyl-L-methionine
-
687808
Mus musculus
?
-
-
-
?
2.1.1.5
L-homocysteine + S-methyl-L-methionine
-
687808
Homo sapiens
?
-
-
-
?
2.1.1.5
L-homocysteine + S-methyl-L-methionine
BHMT-2 uses S-methylmethionine as a methyl donor for the methylation of homocysteine. Unlike BHMT, BHMT-2 can not use betaine
687808
Homo sapiens
?
-
-
-
?
2.1.1.5
L-homocysteine + S-methylmethionine
BHMT-2 uses S-methylmethionine as a methyl donor for the methylation of homocysteine. Unlike BHMT, BHMT-2 can not use betaine
687808
Mus musculus
?
-
-
-
?
2.1.1.10
S-adenosyl-L-methionine + L-homocysteine
low activity
687808
Homo sapiens
?
-
-
-
?
2.1.1.10
S-methyl-L-methionine + L-homocysteine
high homology to betaine-homocysteine methyltransferase (BHMT, EC 2.1.1.5), betaine is not a substrate for BHMT-2 but high activity with S-methyl-L-methionine
687808
Homo sapiens
?
-
-
-
?
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
2.1.1.10
7.5
-
activity assay at
Homo sapiens
Turnover Number [1/s] (protein specific)
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
2.1.1.5
24
-
S-methyl-L-methionine
-
Homo sapiens
2.1.1.5
38
-
S-methyl-L-methionine
-
Homo sapiens
2.1.1.5
88
-
betaine
-
Homo sapiens
2.1.1.10
additional information
-
additional information
similar turnover number like betaine-homocysteine methyltransferase (BHMT, EC 2.1.1.5)
Homo sapiens
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.1.1.5
7.5
-
assay at
Mus musculus