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Literature summary extracted from

  • Cottevieille, M.; Larquet, E.; Jonic, S.; Petoukhov, M.V.; Caprini, G.; Paravisi, S.; Svergun, D.I.; Vanoni, M.A.; Boisset, N.
    The subnanometer resolution structure of the glutamate synthase 1.2-MDa hexamer by cryoelectron microscopy and its oligomerization behavior in solution: functional implications (2008), J. Biol. Chem., 283, 8237-8249.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.4.1.13 expressed in Escherichia coli BL21 (DE3) cells Azospirillum brasilense

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.4.1.13 0.175
-
 NADPH dimeric enzyme, at 25°C in 50 mM HEPES/KOH buffer, pH 7.5, 1 M NaCl, in the presence of 10 mM L-glutamine, and 5 mM 2-oxoglutarate Azospirillum brasilense
1.4.1.13 0.23
-
 2-oxoglutarate dimeric enzyme, at 25°C in 50 mM HEPES/KOH buffer, pH 7.5, 1 M NaCl, in the presence of 10 mM L-glutamine, and 0.1 mM NADPH Azospirillum brasilense
1.4.1.13 0.245
-
 NADPH hexameric enzyme, at 25°C in 50 mM HEPES/KOH buffer, pH 7.5, 1 M NaCl, in the presence of 10 mM L-glutamine, and 5 mM 2-oxoglutarate Azospirillum brasilense
1.4.1.13 0.69
-
 L-glutamine dimeric enzyme, at 25°C in 50 mM HEPES/KOH buffer, pH 7.5, 1 M NaCl, in the presence of 2.5 mM 2-oxoglutarate, and 0.1 mM NADPH Azospirillum brasilense
1.4.1.13 0.75
-
 2-oxoglutarate hexameric enzyme, at 25°C in 50 mM HEPES/KOH buffer, pH 7.5, 1 M NaCl, in the presence of 10 mM L-glutamine, and 0.1 mM NADPH Azospirillum brasilense
1.4.1.13 2.1
-
 L-glutamine hexameric enzyme, at 25°C in 50 mM HEPES/KOH buffer, pH 7.5, 1 M NaCl, in the presence of 2.5 mM 2-oxoglutarate, and 0.1 mM NADPH Azospirillum brasilense

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.4.1.13 200000
-
 2 * 200000, cryoelectron microscopy and small angle X-ray scattering Azospirillum brasilense
1.4.1.13 200000
-
 6 * 200000, cryoelectron microscopy and small angle X-ray scattering, the hexamer exhibits a concentration-dependent equilibrium with monomers and dimers, in solution the hexamer is destabilized by high ionic strength and to a lower extent by the reaction product NADP+ Azospirillum brasilense

Organism

EC Number Organism UniProt Comment Textmining
1.4.1.13 Azospirillum brasilense Q05756 beta subunit
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.4.1.13
-
 Azospirillum brasilense

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.4.1.13 L-glutamine + 2-oxoglutarate + NADPH + H+
-
 Azospirillum brasilense L-glutamate + NADP+
-
 ?

Subunits

EC Number Subunits Comment Organism
1.4.1.13 homodimer 2 * 200000, cryoelectron microscopy and small angle X-ray scattering Azospirillum brasilense
1.4.1.13 homohexamer 6 * 200000, cryoelectron microscopy and small angle X-ray scattering, the hexamer exhibits a concentration-dependent equilibrium with monomers and dimers, in solution the hexamer is destabilized by high ionic strength and to a lower extent by the reaction product NADP+ Azospirillum brasilense

Synonyms

EC Number Synonyms Comment Organism
1.4.1.13 GltS
-
 Azospirillum brasilense