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Literature summary extracted from

  • Zhao, B.; Lin, X.; Lei, L.; Lamb, D.C.; Kelly, S.L.; Waterman, M.R.; Cane, D.E.
    Biosynthesis of the sesquiterpene antibiotic albaflavenone in Streptomyces coelicolor A3(2) (2008), J. Biol. Chem., 283, 8183-8189.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.15.39 coexpression with molecular chaperones GroES/GroEL Streptomyces coelicolor

Protein Variants

EC Number Protein Variants Comment Organism
1.14.15.39 additional information a gene disruption mutant displays no synthesis of both albaflavenone and the albaflavenols, while epi-isozizaene is still produced Streptomyces coelicolor

Organism

EC Number Organism UniProt Comment Textmining
1.14.15.39 Streptomyces coelicolor
-
-
-
1.14.15.39 Streptomyces coelicolor A3(2)
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.15.39 (+)-epi-isozizaene + 2 reduced [2Fe-2S] ferredoxin + 2 H+ + O2
-
 Streptomyces coelicolor (5R,S)-albaflavenol + 2 oxidized [2Fe-2S] ferredoxin + H2O
-
 ?
1.14.15.39 (+)-epi-isozizaene + 2 reduced [2Fe-2S] ferredoxin + 2 H+ + O2
-
 Streptomyces coelicolor A3(2) (5R,S)-albaflavenol + 2 oxidized [2Fe-2S] ferredoxin + H2O
-
 ?
1.14.15.39 (5R,S)-albaflavenol + 2 reduced [2Fe-2S] ferredoxin + 2 H+ + O2
-
 Streptomyces coelicolor albaflavenone + 2 oxidized [2Fe-2S] ferredoxin + 2 H2O
-
 ?
1.14.15.39 (5R,S)-albaflavenol + 2 reduced [2Fe-2S] ferredoxin + 2 H+ + O2
-
 Streptomyces coelicolor A3(2) albaflavenone + 2 oxidized [2Fe-2S] ferredoxin + 2 H2O
-
 ?
1.14.15.39 additional information enzyme catalyses two sequential allylic oxidation reactions. The substrate epi-isozizaene is first oxidized to yield the epimeric intermediates (5R)-albaflavenol and (5S)-albaflavenol, which can be further oxidized to yield the sesquiterpenoid antibiotic albaflavenone. Proposed mechanism of biosynthesis of albaflavenone catalyzed by CYP170A1 Streptomyces coelicolor ?
-
 ?
1.14.15.39 additional information enzyme catalyses two sequential allylic oxidation reactions. The substrate epi-isozizaene is first oxidized to yield the epimeric intermediates (5R)-albaflavenol and (5S)-albaflavenol, which can be further oxidized to yield the sesquiterpenoid antibiotic albaflavenone. Proposed mechanism of biosynthesis of albaflavenone catalyzed by CYP170A1 Streptomyces coelicolor A3(2) ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.14.15.39 CYP170A1
-
 Streptomyces coelicolor

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.15.39 cytochrome P-450
-
 Streptomyces coelicolor