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Literature summary extracted from

  • Pauff, J.M.; Zhang, J.; Bell, C.E.; Hille, R.
    Substrate orientation in xanthine oxidase: crystal structure of enzyme in reaction with 2-hydroxy-6-methylpurine (2008), J. Biol. Chem., 283, 4818-4824.
    View publication on PubMed

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.17.3.2 enzyme in complex with inhibitor 2-hydroxy-6-methylpurine, 10 mg/ml purified enzyme in 40 mM Tris-HCl, pH 7.8, 20 mM diphosphate, pH 8.5, 0.2 mM EDTA and 5 mM DTT, batch method mixing 0.02 ml of protein solution and 0.01 ml of precipitant solution, the latter containing of 12-14% PEG 8000, 0.1 M potassium phosphate, and 0.2 mM EDTA, pH 7.2, for 2-3 days at 25°C, X-ray diffraction structure determination and analysis at 2.3 A resolution, molecular replacement Bos taurus

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.17.3.2 2-hydroxy-6-methylpurine interacts with Arg880 in both active sites of the enzyme dimer, binding structure, overview Bos taurus
1.17.3.2 alloxanthine
-
 Bos taurus
1.17.3.2 FYX-051
-
 Bos taurus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.17.3.2 Molybdenum active site molybdenum center structure, overview Bos taurus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.17.3.2 xanthine + H2O + O2 Bos taurus
-
 urate + H2O2
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.17.3.2 Bos taurus P80457
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.17.3.2 native enzyme partially by gel filtration from fresh unpasteurized milk Bos taurus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.17.3.2 milk
-
 Bos taurus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.17.3.2 xanthine + H2O + O2
-
 Bos taurus urate + H2O2
-
 ?
1.17.3.2 xanthine + H2O + O2 orientation of xanthine in the active site of xanthine oxidoreductase,structure, overview Bos taurus urate + H2O2
-
 ?

Subunits

EC Number Subunits Comment Organism
1.17.3.2 dimer
-
 Bos taurus