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Literature summary extracted from

  • Matsumura, T.; Okamoto, K.; Iwahara, S.; Hori, H.; Takahashi, Y.; Nishino, T.; Abe, Y.
    Dimer-oligomer interconversion of wild-type and mutant rat 2-Cys peroxiredoxin: disulfide formation at dimer-dimer interfaces is not essential for decamerization (2008), J. Biol. Chem., 283, 284-293.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.11.1.24 expressed in Escherichia coli strain JM109 Rattus norvegicus

Protein Variants

EC Number Protein Variants Comment Organism
1.11.1.24 C173S decameric mutant enzyme, cannot form an intermolecular disulfide bridge in the vicinity of the active site under oxidative conditions Rattus norvegicus
1.11.1.24 C52S decameric mutant enzyme, inactive, cannot form an intermolecular disulfide bridge in the vicinity of the active site under oxidative conditions Rattus norvegicus
1.11.1.24 C83S dimeric mutant enzyme, mutant exhibits similar peroxidase activity compared to the wild type enzyme Rattus norvegicus
1.11.1.24 C83S/C173S increased specific activity with dithiothreitol compared to the wild type enzyme, shows no activity with thioredoxin Rattus norvegicus
1.11.1.24 C83S/R128A reduced specific activity compared to the wild type enzyme Rattus norvegicus
1.11.1.24 C83S/R128E reduced specific activity compared to the wild type enzyme Rattus norvegicus
1.11.1.24 C83S/R128K reduced specific activity compared to the wild type enzyme Rattus norvegicus
1.11.1.24 C83S/R151A reduced specific activity compared to the wild type enzyme Rattus norvegicus
1.11.1.24 C83S/R151EA reduced specific activity compared to the wild type enzyme Rattus norvegicus
1.11.1.24 C83S/R151K reduced specific activity compared to the wild type enzyme Rattus norvegicus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.11.1.24 22000
-
 2 * 22000, SDS-PAGE, the protein readily interconverts between dimer and oligomeric forms Rattus norvegicus
1.11.1.24 23000
-
 10 * 23000, SDS-PAGE, the wild type enzyme exists as a mixture of various forms, favoring the homodecamer at higher protein concentration and lower ionic salt concentration and in the presence of dithiothreitol Rattus norvegicus
1.11.1.24 44000
-
 dimer, SDS-PAGE Rattus norvegicus
1.11.1.24 230000
-
 decamer, SDS-PAGE Rattus norvegicus

Organism

EC Number Organism UniProt Comment Textmining
1.11.1.24 Rattus norvegicus Q63716
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.11.1.24 DE-52 column chromatography and 2',5'-ADP-Sepharose affinity column chromatography Rattus norvegicus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.11.1.24 liver
-
 Rattus norvegicus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.11.1.24 0.03
-
 mutant enzyme C83S/R128E, using dithiothreitol as a substrate, in 50 mM HEPES-NaOH (pH 7.0), at 25°C Rattus norvegicus
1.11.1.24 0.03
-
 mutant enzyme C83S/R128E, using thioredoxin as a substrate, in 150 mM potassium phosphate buffer, pH 7.4, at 25°C Rattus norvegicus
1.11.1.24 0.04
-
 mutant enzyme C83S/R128A, using dithiothreitol as a substrate, in 50 mM HEPES-NaOH (pH 7.0), at 25°C Rattus norvegicus
1.11.1.24 0.04
-
 mutant enzyme C83S/R128A, using thioredoxin as a substrate, in 150 mM potassium phosphate buffer, pH 7.4, at 25°C Rattus norvegicus
1.11.1.24 0.04
-
 mutant enzyme C83S/R128K, using dithiothreitol as a substrate, in 50 mM HEPES-NaOH (pH 7.0), at 25°C Rattus norvegicus
1.11.1.24 0.08
-
 mutant enzyme C83S/R128K, using thioredoxin as a substrate, in 150 mM potassium phosphate buffer, pH 7.4, at 25°C Rattus norvegicus
1.11.1.24 0.1
-
 mutant enzyme C83S/R151A, using dithiothreitol as a substrate, in 50 mM HEPES-NaOH (pH 7.0), at 25°C Rattus norvegicus
1.11.1.24 0.1
-
 mutant enzyme C83S/R151E, using dithiothreitol as a substrate, in 50 mM HEPES-NaOH (pH 7.0), at 25°C Rattus norvegicus
1.11.1.24 0.12
-
 mutant enzyme C83S/R151A, using thioredoxin as a substrate, in 150 mM potassium phosphate buffer, pH 7.4, at 25°C Rattus norvegicus
1.11.1.24 0.12
-
 mutant enzyme C83S/R151K, using dithiothreitol as a substrate, in 50 mM HEPES-NaOH (pH 7.0), at 25°C Rattus norvegicus
1.11.1.24 0.18
-
 mutant enzyme C83S/R151E, using thioredoxin as a substrate, in 150 mM potassium phosphate buffer, pH 7.4, at 25°C Rattus norvegicus
1.11.1.24 0.19
-
 mutant enzyme C83S/R151K, using thioredoxin as a substrate, in 150 mM potassium phosphate buffer, pH 7.4, at 25°C Rattus norvegicus
1.11.1.24 0.9
-
 wild type enzyme, using dithiothreitol as a substrate, in 50 mM HEPES-NaOH (pH 7.0), at 25°C Rattus norvegicus
1.11.1.24 1
-
 mutant enzyme C173S, using dithiothreitol as a substrate, in 50 mM HEPES-NaOH (pH 7.0), at 25°C Rattus norvegicus
1.11.1.24 1.07
-
 mutant enzyme C83S, using dithiothreitol as a substrate, in 50 mM HEPES-NaOH (pH 7.0), at 25°C Rattus norvegicus
1.11.1.24 1.95
-
 mutant enzyme C83S/C173S, using dithiothreitol as a substrate, in 50 mM HEPES-NaOH (pH 7.0), at 25°C Rattus norvegicus
1.11.1.24 2.32
-
 mutant enzyme C83S, using thioredoxin as a substrate, in 150 mM potassium phosphate buffer, pH 7.4, at 25°C Rattus norvegicus
1.11.1.24 2.74
-
 wild type enzyme, using thioredoxin as a substrate, in 150 mM potassium phosphate buffer, pH 7.4, at 25°C Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.11.1.24 H2O2 + reduced thioredoxin
-
 Rattus norvegicus H2O + oxidized thioredoxin
-
 ?
1.11.1.24 tert-butyl hydroperoxide + reduced dithiothreitol
-
 Rattus norvegicus tert-butanol + oxidized dithiothreitol
-
 ?

Subunits

EC Number Subunits Comment Organism
1.11.1.24 dimer 2 * 22000, SDS-PAGE, the protein readily interconverts between dimer and oligomeric forms Rattus norvegicus
1.11.1.24 homodecamer 10 * 23000, SDS-PAGE, the wild type enzyme exists as a mixture of various forms, favoring the homodecamer at higher protein concentration and lower ionic salt concentration and in the presence of dithiothreitol Rattus norvegicus

Synonyms

EC Number Synonyms Comment Organism
1.11.1.24 2-Cys peroxiredoxin
-
 Rattus norvegicus
1.11.1.24 HBP23/Prx I
-
 Rattus norvegicus
1.11.1.24 heme-binding protein 23/peroxiredoxin belongs to the 2-Cys peroxiredoxin type I family and exhibits peroxidase activity coupled with reduced thioredoxin as an electron donor Rattus norvegicus
1.11.1.24 Prx I
-
 Rattus norvegicus