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Literature summary extracted from

  • Johnson, E.T.; Schmidt-Dannert, C.
    Characterization of three homologs of the large subunit of the magnesium chelatase from Chlorobaculum tepidum and interaction with the magnesium protoporphyrin IX methyltransferase (2008), J. Biol. Chem., 283, 27776-27784.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.1.1.11 expression in Escherichia coli JM109, constitutive promoter on pUCmod, overexpression improved by using BL21 cells in combination with T7 promoter, subclconing into pET-20b(+) vector, engineered Escherichia coli strain overproducing protoporphyrin for activity assays Chlorobaculum tepidum
6.6.1.1 expression in Escherichia coli Chlorobaculum tepidum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.1.1.11 0.0006
-
 magnesium protoporphyrin concentrations between 0.3 and 30 microM in the presence of 50 nM enzyme Chlorobaculum tepidum
2.1.1.11 0.053
-
 protoporphyrin concentrations between 1 and 30 microM in the presence of 200 nM enzyme Chlorobaculum tepidum
6.6.1.1 0.0006
-
 protoporphyrin IX complex of subunits BchD, BchI, BchH2, pH 8.0, 34°C Chlorobaculum tepidum
6.6.1.1 0.0012
-
 protoporphyrin IX complex of subunits BchD, BchI, BchH-1, pH 8.0, 34°C Chlorobaculum tepidum
6.6.1.1 0.0012
-
 protoporphyrin IX complex of subunits BchD, BchI, BchH1, pH 8.0, 34°C Chlorobaculum tepidum

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
6.6.1.1 Mg2+ K0.5 value 8.6 mM Chlorobaculum tepidum
6.6.1.1 Mg2+ K0.5 value 9.1 mM Chlorobaculum tepidum

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.11 Chlorobaculum tepidum
-
 formerly Chlorobium tepidum
-
6.6.1.1 Chlorobaculum tepidum Q8KB39 isoform BchH1
-
6.6.1.1 Chlorobaculum tepidum Q93SV6 isoform BchH2
-
6.6.1.1 Chlorobaculum tepidum Q93SV9 isoform BchH3
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.1.1.11 recombinant protein, gel filtration and SDS-PAGE Chlorobaculum tepidum

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.1.1.11 cell culture
-
 Chlorobaculum tepidum
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.1.1.11 additional information
-
 activity assay in protoporphyrin overproducing Escherichia coli cells, interaction of subunits of the three magnesium chelatase complexes with magnesium protoporphyrin methyltransferase, differences in subunit interactions either increase or decrease methyltransferase activity, vice versa, activity of any of the three magnesium chelatase complexes not affected Chlorobaculum tepidum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.11 S-adenosyl-L-methionine + magnesium protoporphyrin different substrate specificity, protoporphyrin with or without bound metal as a substrate Chlorobaculum tepidum S-adenosyl-L-homocysteine + magnesium protoporphyrin monomethyl ester
-
 ?
2.1.1.11 S-adenosyl-L-methionine + protoporphyrin methyl transfer possible without bound metal, slow reaction rate Chlorobaculum tepidum S-adenosyl-L-homocysteine + protoporphyrin monomethyl ester
-
 ?
6.6.1.1 ATP + protoporphyrin IX + Mg2+ + H2O
-
 Chlorobaculum tepidum ADP + phosphate + Mg-protoporphyrin IX + H+
-
 ?

Subunits

EC Number Subunits Comment Organism
2.1.1.11 More majority of the protein exist as a high-molecular-weight multimer as determined by gel filtration Chlorobaculum tepidum
6.6.1.1 More enzyme interacts with the next enzyme in the pathway, magnesium protoporphyrine IX methyltransferase BchM. Activitiy of BchM in presence of isoform BchH1 is 132% of the activity of BchM alone Chlorobaculum tepidum
6.6.1.1 More enzyme interacts with the next enzyme in the pathway, magnesium protoporphyrine IX methyltransferase BchM. Activitiy of BchM in presence of isoform BchH2 is 157% of the activity of BchM alone Chlorobaculum tepidum
6.6.1.1 More enzyme interacts with the next enzyme in the pathway, magnesium protoporphyrine IX methyltransferase BchM. Activitiy of BchM in presence of isoform BchH3 is 68% of the activity of BchM alone Chlorobaculum tepidum

Synonyms

EC Number Synonyms Comment Organism
2.1.1.11 BchM
-
 Chlorobaculum tepidum

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.1.1.11 30
-
 assay at Chlorobaculum tepidum

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.1.1.11 0.0002
-
 protoporphyrin concentrations between 1 and 30 microM in the presence of 200 nM enzyme Chlorobaculum tepidum
2.1.1.11 0.14
-
 magnesium protoporphyrin concentrations between 0.3 and 30 microM in the presence of 50 nM enzyme Chlorobaculum tepidum
6.6.1.1 0.0003
-
 protoporphyrin IX complex of subunits BchD, BchI, BchH1, pH 8.0, 34°C Chlorobaculum tepidum
6.6.1.1 0.0003
-
 protoporphyrin IX complex of subunits BchD, BchI, BchH3, pH 8.0, 34°C Chlorobaculum tepidum
6.6.1.1 0.03
-
 protoporphyrin IX complex of subunits BchD, BchI, BchH2, pH 8.0, 34°C Chlorobaculum tepidum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.1.1.11 7.5
-
 assay at Chlorobaculum tepidum