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Literature summary extracted from

  • Oakley, A.J.; Yamada, T.; Liu, D.; Coggan, M.; Clark, A.G.; Board, P.G.
    The identification and structural characterization of C7orf24 as gamma-glutamyl cyclotransferase. An essential enzyme in the gamma-glutamyl cycle (2008), J. Biol. Chem., 283, 22031-22042.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.3.2.9
-
 Homo sapiens

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
4.3.2.9 structure is determined at 1.7 A resolution Homo sapiens

Protein Variants

EC Number Protein Variants Comment Organism
4.3.2.9 E98A inactive mutant enzyme Homo sapiens
4.3.2.9 E98Q inactive mutant enzyme Homo sapiens
4.3.2.9 G23A kcat/KM for gamma-glutamyl-L-alanine is 8.4fold lower than wild-type value Homo sapiens
4.3.2.9 Y105F kcat/KM for gamma-glutamyl-L-alanine is 650fold lower than wild-type value Homo sapiens
4.3.2.9 Y125F kcat/KM for gamma-glutamyl-L-alanine is 3.7fold lower than wild-type value Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.3.2.9 2
-
 gamma-glutamyl-L-alanine recombinant wild type enzyme Homo sapiens
4.3.2.9 5.6
-
 gamma-glutamyl-L-alanine mutant enzyme Y105F Homo sapiens
4.3.2.9 5.8
-
 gamma-glutamyl-L-alanine mutant enzyme Y125F Homo sapiens
4.3.2.9 8
-
 gamma-glutamyl-L-alanine mutant enzyme G23A Homo sapiens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.3.2.9 20994
-
 x * 20994, calculated from sequence Homo sapiens
4.3.2.9 21000
-
 x * 21000, recombinant enzyme, SDS-PAGE Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
4.3.2.9 Homo sapiens O75223
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.3.2.9
-
 Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
4.3.2.9 bladder high concentration of GGCT transcripts Homo sapiens
-
4.3.2.9 blood
-
 Homo sapiens
-
4.3.2.9 bone marrow
-
 Homo sapiens
-
4.3.2.9 brain
-
 Homo sapiens
-
4.3.2.9 breast cancer cell
-
 Homo sapiens
-
4.3.2.9 colon
-
 Homo sapiens
-
4.3.2.9 colonic cancer cell
-
 Homo sapiens
-
4.3.2.9 erythrocyte
-
 Homo sapiens
-
4.3.2.9 esophagus
-
 Homo sapiens
-
4.3.2.9 heart
-
 Homo sapiens
-
4.3.2.9 kidney
-
 Homo sapiens
-
4.3.2.9 liver
-
 Homo sapiens
-
4.3.2.9 lung
-
 Homo sapiens
-
4.3.2.9 mammary gland
-
 Homo sapiens
-
4.3.2.9 muscle
-
 Homo sapiens
-
4.3.2.9 ovary
-
 Homo sapiens
-
4.3.2.9 pancreas
-
 Homo sapiens
-
4.3.2.9 placenta
-
 Homo sapiens
-
4.3.2.9 prostate cancer cell
-
 Homo sapiens
-
4.3.2.9 prostate gland
-
 Homo sapiens
-
4.3.2.9 renal cancer cell
-
 Homo sapiens
-
4.3.2.9 salivary gland high concentration of GGCT transcripts Homo sapiens
-
4.3.2.9 skin
-
 Homo sapiens
-
4.3.2.9 small intestine
-
 Homo sapiens
-
4.3.2.9 spleen
-
 Homo sapiens
-
4.3.2.9 stomach
-
 Homo sapiens
-
4.3.2.9 testis
-
 Homo sapiens
-
4.3.2.9 tonsil
-
 Homo sapiens
-
4.3.2.9 urinary bladder cancer cell high concentration of GGCT transcripts Homo sapiens
-
4.3.2.9 uterus
-
 Homo sapiens
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.3.2.9 0.0503
-
 recombinant enzyme Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.3.2.9 gamma-glutamyl-L-alanine
-
 Homo sapiens 5-oxoproline + alanine
-
 ?

Subunits

EC Number Subunits Comment Organism
4.3.2.9 ? x * 21000, recombinant enzyme, SDS-PAGE Homo sapiens
4.3.2.9 ? x * 20994, calculated from sequence Homo sapiens

Synonyms

EC Number Synonyms Comment Organism
4.3.2.9 C7orf24
-
 Homo sapiens
4.3.2.9 GGCT
-
 Homo sapiens

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4.3.2.9 0.08
-
 gamma-glutamyl-L-alanine mutant enzyme Y105F Homo sapiens
4.3.2.9 6.6
-
 gamma-glutamyl-L-alanine mutant enzyme G23A Homo sapiens
4.3.2.9 14.7
-
 gamma-glutamyl-L-alanine mutant enzyme Y125F Homo sapiens
4.3.2.9 18.3
-
 gamma-glutamyl-L-alanine recombinant wild type enzyme Homo sapiens