EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.14.13.39 | additional information | - |
additional information | kinetics of cytcochrome c reduction and of reaction with FMN and cytochrome c, overview, eNOS and nNOS differ slightly in their NADP(H) interaction and flavin thermodynamics, and show distinct electron transfer activities, two-state conformational equilibrium of the FMN subdomain, overview | Bos taurus |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.14.13.39 | 2 L-arginine + 3 NADPH + 3 H+ + 4 O2 | Bos taurus | - |
2 citrulline + 2 nitric oxide + 3 NADP+ + 4 H2O | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.14.13.39 | Bos taurus | - |
isozymes eNOS and nNOS | - |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
1.14.13.39 | endothelium | - |
Bos taurus | - |
1.14.13.39 | neuron | - |
Bos taurus | - |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
1.14.13.39 | additional information | - |
low eNOS activity might involve an altered interaction with NADP(H) | Bos taurus |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.14.13.39 | 2 L-arginine + 3 NADPH + 3 H+ + 4 O2 | - |
Bos taurus | 2 citrulline + 2 nitric oxide + 3 NADP+ + 4 H2O | - |
? | |
1.14.13.39 | 2 L-arginine + 3 NADPH + 3 H+ + 4 O2 | the electron transfer between cofactors FMN and FAD is reversible | Bos taurus | 2 citrulline + 2 nitric oxide + 3 NADP+ + 4 H2O | - |
? | |
1.14.13.39 | additional information | endothelial NOS has a 6fold lower NO synthesis activity and 6-16fold lower cytochrome c reductase activity than neuronal NOS due to a significantly different electron transfer capacities, substrate specificity and mechanism, oveview | Bos taurus | ? | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.14.13.39 | endothelial nitric-oxide synthase | - |
Bos taurus |
1.14.13.39 | endothelial NOS | - |
Bos taurus |
1.14.13.39 | eNOS | - |
Bos taurus |
1.14.13.39 | neuronal nitric-oxide synthase | - |
Bos taurus |
1.14.13.39 | neuronal NOS | - |
Bos taurus |
1.14.13.39 | nNOS | - |
Bos taurus |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.14.13.39 | 10 | 25 | assay at | Bos taurus |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.14.13.39 | 7.6 | - |
assay at | Bos taurus |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.14.13.39 | cytochrome c | - |
Bos taurus | |
1.14.13.39 | FAD | - |
Bos taurus | |
1.14.13.39 | FMN | - |
Bos taurus | |
1.14.13.39 | NADP+ | binding mechanism | Bos taurus | |
1.14.13.39 | NADPH | binding mechanism | Bos taurus |