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Literature summary extracted from

  • Di Costanzo, L.; Drury, J.E.; Penning, T.M.; Christianson, D.W.
    Crystal structure of human liver DELTA4-3-ketosteroid 5beta-reductase (AKR1D1) and implications for substrate binding and catalysis (2008), J. Biol. Chem., 283, 16830-16839.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.3.1.3 expressed in Escherichia coli strain C41(DE3) Homo sapiens

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.3.1.3 hanging drop vapour diffusion method, in 0.1 M Tris/HEPES (pH 7.5), 10-20% (w/v) polyethylene glycol 4000, and 10% isopropyl alcohol Homo sapiens

Protein Variants

EC Number Protein Variants Comment Organism
1.3.1.3 E120A mutant is devoid of activity Homo sapiens
1.3.1.3 Y58F mutant is devoid of activity Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.3.1.3 testosterone
-
 Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.3.1.3 0.0027
-
 testosterone in 100 mM potassium phosphate buffer (pH 6.0), at 37°C Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
1.3.1.3 Homo sapiens
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.3.1.3 Ni2+-Sepharose column chromatography Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.3.1.3 liver
-
 Homo sapiens
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.3.1.3 cortisone + NADPH + H+
-
 Homo sapiens 5beta-17,21dihydroxy-pregnan-3,11,20-trione + NADP+
-
 ?
1.3.1.3 progesterone + NADPH + H+
-
 Homo sapiens 5beta-pregnane-3,20-dione + NADP+
-
 ?
1.3.1.3 testosterone + NADPH + H+
-
 Homo sapiens (5beta,17beta)-17-hydroxyandrostan-3-one + NADP+
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.3.1.3 AKR1D1
-
 Homo sapiens
1.3.1.3 DELTA4-3-ketosteroid 5beta-reductase
-
 Homo sapiens
1.3.1.3 steroid 5beta-reductase
-
 Homo sapiens

Cofactor

EC Number Cofactor Comment Organism Structure
1.3.1.3 NADPH
-
 Homo sapiens

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.3.1.3 0.0145
-
 testosterone in 100 mM potassium phosphate buffer (pH 6.0), at 37°C Homo sapiens