BRENDA - Enzyme Database

Substrate specificity and membrane topology of Escherichia coli PgpB, an undecaprenyl pyrophosphate phosphatase

Touze, T.; Blanot, D.; Mengin-Lecreulx, D.; J. Biol. Chem. 283, 16573-16583 (2008)

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
3.6.1.27
cardiolipin
-
Escherichia coli
3.6.1.27
diacylglycerol diphosphate
increases the activity with undecaprenyl diphosphate by about 400%
Escherichia coli
3.6.1.27
phosphatidylglycerol
-
Escherichia coli
3.6.1.27
Phospholipids
-
Escherichia coli
Cloned(Commentary)
EC Number
Commentary
Organism
3.6.1.27
gene pgpB, overexpression of His-tagged enzyme in strain DH5alpha and C43
Escherichia coli
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.6.1.27
0.08
-
diacylglycerol diphosphate
pH 7.5, 37°C, recombinant enzyme
Escherichia coli
3.6.1.27
0.096
-
farnesyl diphosphate
pH 7.5, 37°C, recombinant enzyme
Escherichia coli
3.6.1.27
0.53
-
undecaprenyl diphosphate
pH 7.5, 37°C, recombinant enzyme
Escherichia coli
3.6.1.27
1.7
-
phosphatidic acid
pH 7.5, 37°C, recombinant enzyme
Escherichia coli
3.6.1.27
3.6
-
isopentenyl diphosphate
pH 7.5, 37°C, recombinant enzyme
Escherichia coli
3.6.1.27
3.9
-
diphosphate
pH 7.5, 37°C, recombinant enzyme
Escherichia coli
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
3.6.1.27
membrane
integral, PgpB contains six transmembrane segments, a large periplasmic loop, and the type 2 phosphatidic acid phosphatase signature residues at a periplasmic location
Escherichia coli
16020
-
3.6.1.27
additional information
topological mapping, modelling, overview
Escherichia coli
-
-
3.6.1.27
periplasm
PgpB contains six transmembrane segments, a large periplasmic loop, and the type 2 phosphatidic acid phosphatase signature residues at a periplasmic location
Escherichia coli
-
-
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.6.1.27
30000
-
x * 30158, mass spectrometry, x * 30000, SDS-PAGE
Escherichia coli
3.6.1.27
30158
-
x * 30158, mass spectrometry, x * 30000, SDS-PAGE
Escherichia coli
3.6.1.27
110000
-
recombinant His-tagged enzyme, gel filtration
Escherichia coli
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3.6.1.27
undecaprenyl diphosphate + H2O
Escherichia coli
the synthesis of the lipid carrier undecaprenyl phosphate, C55-P, requires the dephosphorylation of its precursor, undecaprenyl pyrophosphate, C55-PP, the dephosphorylation involves four integral membrane proteins, BacA, and three members of the type 2 phosphatidic acid phosphatase family, PgpB, YbjG, and YeiU, overview
undecaprenyl phosphate + phosphate
-
-
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
3.6.1.27
Escherichia coli
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
3.6.1.27
recombinant His-tagged enzyme from membranes to homogeneity by ultracentrifugation, nickel affinity chromatography
Escherichia coli
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
3.6.1.27
1
-
purified recombinant enzyme, substrate isopentenyl diphosphate
Escherichia coli
3.6.1.27
3
-
purified recombinant enzyme, substrate undecaprenyl diphosphate
Escherichia coli
3.6.1.27
6.7
-
purified recombinant enzyme, substrate phosphatidic acid
Escherichia coli
3.6.1.27
300
-
purified recombinant enzyme, substrate farnesyl diphosphate
Escherichia coli
3.6.1.27
310
-
purified recombinant enzyme, substrate diacylglycerol diphosphate
Escherichia coli
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.6.1.27
diacylglycerol diphosphate + H2O
-
687718
Escherichia coli
diacylglycerol phosphate + phosphate
-
-
-
?
3.6.1.27
diphosphate + H2O
low activity
687718
Escherichia coli
2 phosphate
-
-
-
?
3.6.1.27
farnesyl diphosphate + H2O
-
687718
Escherichia coli
farnesyl phosphate + phosphate
-
-
-
?
3.6.1.27
isopentenyl diphosphate + H2O
low activity
687718
Escherichia coli
isopentenyl phosphate + phosphate
-
-
-
?
3.6.1.27
additional information
substrate specificity, overview
687718
Escherichia coli
?
-
-
-
-
3.6.1.27
phosphatidic acid + H2O
low activity
687718
Escherichia coli
?
-
-
-
?
3.6.1.27
undecaprenyl diphosphate + H2O
low activity
687718
Escherichia coli
undecaprenyl phosphate + phosphate
-
-
-
?
3.6.1.27
undecaprenyl diphosphate + H2O
the synthesis of the lipid carrier undecaprenyl phosphate, C55-P, requires the dephosphorylation of its precursor, undecaprenyl pyrophosphate, C55-PP, the dephosphorylation involves four integral membrane proteins, BacA, and three members of the type 2 phosphatidic acid phosphatase family, PgpB, YbjG, and YeiU, overview
687718
Escherichia coli
undecaprenyl phosphate + phosphate
-
-
-
?
Subunits
EC Number
Subunits
Commentary
Organism
3.6.1.27
More
the enzyme exists as a monomer in n-dodecyl-beta-D-maltoside solution, topological mapping, overview
Escherichia coli
3.6.1.27
oligomer
x * 30158, mass spectrometry, x * 30000, SDS-PAGE
Escherichia coli
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
3.6.1.27
37
-
assay at
Escherichia coli
Turnover Number [1/s]
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
3.6.1.27
9
-
undecaprenyl diphosphate
pH 7.5, 37°C, recombinant enzyme
Escherichia coli
3.6.1.27
19
-
isopentenyl diphosphate
pH 7.5, 37°C, recombinant enzyme
Escherichia coli
3.6.1.27
48
-
diphosphate
pH 7.5, 37°C, recombinant enzyme
Escherichia coli
3.6.1.27
61
-
phosphatidic acid
pH 7.5, 37°C, recombinant enzyme
Escherichia coli
3.6.1.27
280
-
diacylglycerol diphosphate
pH 7.5, 37°C, recombinant enzyme
Escherichia coli
3.6.1.27
290
-
farnesyl diphosphate
pH 7.5, 37°C, recombinant enzyme
Escherichia coli
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.6.1.27
6.5
7.5
-
Escherichia coli
pH Range
EC Number
pH Minimum
pH Maximum
Commentary
Organism
3.6.1.27
5
9
50% of maximal activity at pH values other than pH 6.5-pH 7.5
Escherichia coli
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
3.6.1.27
cardiolipin
-
Escherichia coli
3.6.1.27
diacylglycerol diphosphate
increases the activity with undecaprenyl diphosphate by about 400%
Escherichia coli
3.6.1.27
phosphatidylglycerol
-
Escherichia coli
3.6.1.27
Phospholipids
-
Escherichia coli
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
3.6.1.27
gene pgpB, overexpression of His-tagged enzyme in strain DH5alpha and C43
Escherichia coli
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.6.1.27
0.08
-
diacylglycerol diphosphate
pH 7.5, 37°C, recombinant enzyme
Escherichia coli
3.6.1.27
0.096
-
farnesyl diphosphate
pH 7.5, 37°C, recombinant enzyme
Escherichia coli
3.6.1.27
0.53
-
undecaprenyl diphosphate
pH 7.5, 37°C, recombinant enzyme
Escherichia coli
3.6.1.27
1.7
-
phosphatidic acid
pH 7.5, 37°C, recombinant enzyme
Escherichia coli
3.6.1.27
3.6
-
isopentenyl diphosphate
pH 7.5, 37°C, recombinant enzyme
Escherichia coli
3.6.1.27
3.9
-
diphosphate
pH 7.5, 37°C, recombinant enzyme
Escherichia coli
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
3.6.1.27
membrane
integral, PgpB contains six transmembrane segments, a large periplasmic loop, and the type 2 phosphatidic acid phosphatase signature residues at a periplasmic location
Escherichia coli
16020
-
3.6.1.27
additional information
topological mapping, modelling, overview
Escherichia coli
-
-
3.6.1.27
periplasm
PgpB contains six transmembrane segments, a large periplasmic loop, and the type 2 phosphatidic acid phosphatase signature residues at a periplasmic location
Escherichia coli
-
-
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.6.1.27
30000
-
x * 30158, mass spectrometry, x * 30000, SDS-PAGE
Escherichia coli
3.6.1.27
30158
-
x * 30158, mass spectrometry, x * 30000, SDS-PAGE
Escherichia coli
3.6.1.27
110000
-
recombinant His-tagged enzyme, gel filtration
Escherichia coli
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3.6.1.27
undecaprenyl diphosphate + H2O
Escherichia coli
the synthesis of the lipid carrier undecaprenyl phosphate, C55-P, requires the dephosphorylation of its precursor, undecaprenyl pyrophosphate, C55-PP, the dephosphorylation involves four integral membrane proteins, BacA, and three members of the type 2 phosphatidic acid phosphatase family, PgpB, YbjG, and YeiU, overview
undecaprenyl phosphate + phosphate
-
-
?
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
3.6.1.27
recombinant His-tagged enzyme from membranes to homogeneity by ultracentrifugation, nickel affinity chromatography
Escherichia coli
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
3.6.1.27
1
-
purified recombinant enzyme, substrate isopentenyl diphosphate
Escherichia coli
3.6.1.27
3
-
purified recombinant enzyme, substrate undecaprenyl diphosphate
Escherichia coli
3.6.1.27
6.7
-
purified recombinant enzyme, substrate phosphatidic acid
Escherichia coli
3.6.1.27
300
-
purified recombinant enzyme, substrate farnesyl diphosphate
Escherichia coli
3.6.1.27
310
-
purified recombinant enzyme, substrate diacylglycerol diphosphate
Escherichia coli
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.6.1.27
diacylglycerol diphosphate + H2O
-
687718
Escherichia coli
diacylglycerol phosphate + phosphate
-
-
-
?
3.6.1.27
diphosphate + H2O
low activity
687718
Escherichia coli
2 phosphate
-
-
-
?
3.6.1.27
farnesyl diphosphate + H2O
-
687718
Escherichia coli
farnesyl phosphate + phosphate
-
-
-
?
3.6.1.27
isopentenyl diphosphate + H2O
low activity
687718
Escherichia coli
isopentenyl phosphate + phosphate
-
-
-
?
3.6.1.27
additional information
substrate specificity, overview
687718
Escherichia coli
?
-
-
-
-
3.6.1.27
phosphatidic acid + H2O
low activity
687718
Escherichia coli
?
-
-
-
?
3.6.1.27
undecaprenyl diphosphate + H2O
low activity
687718
Escherichia coli
undecaprenyl phosphate + phosphate
-
-
-
?
3.6.1.27
undecaprenyl diphosphate + H2O
the synthesis of the lipid carrier undecaprenyl phosphate, C55-P, requires the dephosphorylation of its precursor, undecaprenyl pyrophosphate, C55-PP, the dephosphorylation involves four integral membrane proteins, BacA, and three members of the type 2 phosphatidic acid phosphatase family, PgpB, YbjG, and YeiU, overview
687718
Escherichia coli
undecaprenyl phosphate + phosphate
-
-
-
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
3.6.1.27
More
the enzyme exists as a monomer in n-dodecyl-beta-D-maltoside solution, topological mapping, overview
Escherichia coli
3.6.1.27
oligomer
x * 30158, mass spectrometry, x * 30000, SDS-PAGE
Escherichia coli
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
3.6.1.27
37
-
assay at
Escherichia coli
Turnover Number [1/s] (protein specific)
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
3.6.1.27
9
-
undecaprenyl diphosphate
pH 7.5, 37°C, recombinant enzyme
Escherichia coli
3.6.1.27
19
-
isopentenyl diphosphate
pH 7.5, 37°C, recombinant enzyme
Escherichia coli
3.6.1.27
48
-
diphosphate
pH 7.5, 37°C, recombinant enzyme
Escherichia coli
3.6.1.27
61
-
phosphatidic acid
pH 7.5, 37°C, recombinant enzyme
Escherichia coli
3.6.1.27
280
-
diacylglycerol diphosphate
pH 7.5, 37°C, recombinant enzyme
Escherichia coli
3.6.1.27
290
-
farnesyl diphosphate
pH 7.5, 37°C, recombinant enzyme
Escherichia coli
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.6.1.27
6.5
7.5
-
Escherichia coli
pH Range (protein specific)
EC Number
pH Minimum
pH Maximum
Commentary
Organism
3.6.1.27
5
9
50% of maximal activity at pH values other than pH 6.5-pH 7.5
Escherichia coli