Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Touze, T.; Blanot, D.; Mengin-Lecreulx, D.
    Substrate specificity and membrane topology of Escherichia coli PgpB, an undecaprenyl pyrophosphate phosphatase (2008), J. Biol. Chem., 283, 16573-16583.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.6.1.27 cardiolipin
-
 Escherichia coli
3.6.1.27 diacylglycerol diphosphate increases the activity with undecaprenyl diphosphate by about 400% Escherichia coli
3.6.1.27 phosphatidylglycerol
-
 Escherichia coli
3.6.1.27 Phospholipids
-
 Escherichia coli

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.6.1.27 gene pgpB, overexpression of His-tagged enzyme in strain DH5alpha and C43 Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.6.1.27 0.08
-
 diacylglycerol diphosphate pH 7.5, 37°C, recombinant enzyme Escherichia coli
3.6.1.27 0.096
-
 farnesyl diphosphate pH 7.5, 37°C, recombinant enzyme Escherichia coli
3.6.1.27 0.53
-
 undecaprenyl diphosphate pH 7.5, 37°C, recombinant enzyme Escherichia coli
3.6.1.27 1.7
-
 phosphatidic acid pH 7.5, 37°C, recombinant enzyme Escherichia coli
3.6.1.27 3.6
-
 isopentenyl diphosphate pH 7.5, 37°C, recombinant enzyme Escherichia coli
3.6.1.27 3.9
-
 diphosphate pH 7.5, 37°C, recombinant enzyme Escherichia coli

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.6.1.27 membrane integral, PgpB contains six transmembrane segments, a large periplasmic loop, and the type 2 phosphatidic acid phosphatase signature residues at a periplasmic location Escherichia coli 16020
-
3.6.1.27 additional information topological mapping, modelling, overview Escherichia coli
-
-
3.6.1.27 periplasm PgpB contains six transmembrane segments, a large periplasmic loop, and the type 2 phosphatidic acid phosphatase signature residues at a periplasmic location Escherichia coli
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.6.1.27 30000
-
 x * 30158, mass spectrometry, x * 30000, SDS-PAGE Escherichia coli
3.6.1.27 30158
-
 x * 30158, mass spectrometry, x * 30000, SDS-PAGE Escherichia coli
3.6.1.27 110000
-
 recombinant His-tagged enzyme, gel filtration Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.6.1.27 undecaprenyl diphosphate + H2O Escherichia coli the synthesis of the lipid carrier undecaprenyl phosphate, C55-P, requires the dephosphorylation of its precursor, undecaprenyl pyrophosphate, C55-PP, the dephosphorylation involves four integral membrane proteins, BacA, and three members of the type 2 phosphatidic acid phosphatase family, PgpB, YbjG, and YeiU, overview undecaprenyl phosphate + phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.6.1.27 Escherichia coli
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.6.1.27 recombinant His-tagged enzyme from membranes to homogeneity by ultracentrifugation, nickel affinity chromatography Escherichia coli

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.6.1.27 1
-
 purified recombinant enzyme, substrate isopentenyl diphosphate Escherichia coli
3.6.1.27 3
-
 purified recombinant enzyme, substrate undecaprenyl diphosphate Escherichia coli
3.6.1.27 6.7
-
 purified recombinant enzyme, substrate phosphatidic acid Escherichia coli
3.6.1.27 300
-
 purified recombinant enzyme, substrate farnesyl diphosphate Escherichia coli
3.6.1.27 310
-
 purified recombinant enzyme, substrate diacylglycerol diphosphate Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.6.1.27 diacylglycerol diphosphate + H2O
-
 Escherichia coli diacylglycerol phosphate + phosphate
-
 ?
3.6.1.27 diphosphate + H2O low activity Escherichia coli 2 phosphate
-
 ?
3.6.1.27 farnesyl diphosphate + H2O
-
 Escherichia coli farnesyl phosphate + phosphate
-
 ?
3.6.1.27 isopentenyl diphosphate + H2O low activity Escherichia coli isopentenyl phosphate + phosphate
-
 ?
3.6.1.27 additional information substrate specificity, overview Escherichia coli ?
-
 ?
3.6.1.27 phosphatidic acid + H2O low activity Escherichia coli ?
-
 ?
3.6.1.27 undecaprenyl diphosphate + H2O low activity Escherichia coli undecaprenyl phosphate + phosphate
-
 ?
3.6.1.27 undecaprenyl diphosphate + H2O the synthesis of the lipid carrier undecaprenyl phosphate, C55-P, requires the dephosphorylation of its precursor, undecaprenyl pyrophosphate, C55-PP, the dephosphorylation involves four integral membrane proteins, BacA, and three members of the type 2 phosphatidic acid phosphatase family, PgpB, YbjG, and YeiU, overview Escherichia coli undecaprenyl phosphate + phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
3.6.1.27 More the enzyme exists as a monomer in n-dodecyl-beta-D-maltoside solution, topological mapping, overview Escherichia coli
3.6.1.27 oligomer x * 30158, mass spectrometry, x * 30000, SDS-PAGE Escherichia coli

Synonyms

EC Number Synonyms Comment Organism
3.6.1.27 More the enzyme is a member of the type 2 phosphatidic acid phosphatase family Escherichia coli
3.6.1.27 PgpB
-
 Escherichia coli
3.6.1.27 undecaprenyl pyrophosphate phosphatase
-
 Escherichia coli

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.6.1.27 37
-
 assay at Escherichia coli

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.6.1.27 9
-
 undecaprenyl diphosphate pH 7.5, 37°C, recombinant enzyme Escherichia coli
3.6.1.27 19
-
 isopentenyl diphosphate pH 7.5, 37°C, recombinant enzyme Escherichia coli
3.6.1.27 48
-
 diphosphate pH 7.5, 37°C, recombinant enzyme Escherichia coli
3.6.1.27 61
-
 phosphatidic acid pH 7.5, 37°C, recombinant enzyme Escherichia coli
3.6.1.27 280
-
 diacylglycerol diphosphate pH 7.5, 37°C, recombinant enzyme Escherichia coli
3.6.1.27 290
-
 farnesyl diphosphate pH 7.5, 37°C, recombinant enzyme Escherichia coli

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.6.1.27 6.5 7.5
-
 Escherichia coli

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.6.1.27 5 9 50% of maximal activity at pH values other than pH 6.5-pH 7.5 Escherichia coli