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Literature summary extracted from

  • Massengo-Tiasse, R.P.; Cronan, J.E.
    Vibrio cholerae FabV defines a new class of enoyl-acyl carrier protein reductase (2008), J. Biol. Chem., 283, 1308-1316.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.3.1.9 expressed in Escherichia coli BL21 Star (DE3) cells Vibrio cholerae serotype O1

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.3.1.9 additional information the enzyme is very resistant to triclosan Vibrio cholerae serotype O1

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.3.1.9 0.053
-
 NADH native enzyme, in 0.1 M sodium phosphate buffer (pH 7) Vibrio cholerae serotype O1
1.3.1.9 0.195
-
 crotonyl-[acyl-carrier protein] native enzyme, in 0.1 M sodium phosphate buffer (pH 7) Vibrio cholerae serotype O1
1.3.1.9 1.178
-
 crotonyl-CoA native enzyme, in 0.1 M sodium phosphate buffer (pH 7) Vibrio cholerae serotype O1
1.3.1.9 3.043
-
 NADPH native enzyme, in 0.1 M sodium phosphate buffer (pH 7) Vibrio cholerae serotype O1

Organism

EC Number Organism UniProt Comment Textmining
1.3.1.9 Vibrio cholerae serotype O1
-
 strain ATCC 14547
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.3.1.9 Ni-NTA column chromatography, Vivapure D maxi H spin column chromatography, and Blue Sepharose column chromatography Vibrio cholerae serotype O1

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.3.1.9 crotonyl-CoA + NADH + H+
-
 Vibrio cholerae serotype O1 butyryl-CoA + NAD+
-
 ?
1.3.1.9 crotonyl-CoA + NADPH + H+ the native enzyme shows very weak activity with NADPH Vibrio cholerae serotype O1 butyryl-CoA + NADP+
-
 ?
1.3.1.9 crotonyl-[acyl-carrier protein] + NADH
-
 Vibrio cholerae serotype O1 butyryl-[acyl-carrier protein] + NAD+
-
 ?

Subunits

EC Number Subunits Comment Organism
1.3.1.9 monomer gel filtration Vibrio cholerae serotype O1

Synonyms

EC Number Synonyms Comment Organism
1.3.1.9 enoyl-ACP reductase
-
 Vibrio cholerae serotype O1
1.3.1.9 enoyl-acyl carrier protein reductase
-
 Vibrio cholerae serotype O1
1.3.1.9 FabV
-
 Vibrio cholerae serotype O1

Cofactor

EC Number Cofactor Comment Organism Structure
1.3.1.9 NADH shows a very strong preference for NADH over NADPH Vibrio cholerae serotype O1