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Literature summary extracted from

  • Wei, C.C.; Wang, Z.Q.; Tejero, J.; Yang, Y.P.; Hemann, C.; Hille, R.; Stuehr, D.J.
    Catalytic reduction of a tetrahydrobiopterin radical within nitric-oxide synthase (2008), J. Biol. Chem., 283, 11734-11742.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.13.39 expression of nNOS in Escherichia coli Rattus norvegicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.14.13.39 additional information
-
 additional information kinetics, rapid-freeze method, overview Rattus norvegicus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.14.13.39 Ca2+ dependent on Rattus norvegicus
1.14.13.39 Fe2+ a heme enzyme Rattus norvegicus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.13.39 2 L-arginine + 3 NADPH + 3 H+ + 4 O2 Rattus norvegicus
-
 2 citrulline + 2 nitric oxide + 3 NADP+ + 4 H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.14.13.39 Rattus norvegicus P29476
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.14.13.39 recombinant nNOS from Escherichia coli in absence of tetrahydrobiopterin Rattus norvegicus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.14.13.39 neuron
-
 Rattus norvegicus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.13.39 2 L-arginine + 2 NADPH + 2 H+ + 2 O2 first half reaction via intermediate Nomega-hydroxy-L-arginine Rattus norvegicus 2 Nomega-hydroxy-L-arginine + 2 NADP+ + 2 H2O
-
 ?
1.14.13.39 2 L-arginine + 3 NADPH + 3 H+ + 4 O2
-
 Rattus norvegicus 2 citrulline + 2 nitric oxide + 3 NADP+ + 4 H2O
-
 ?
1.14.13.39 2 L-arginine + 3 NADPH + 3 H+ + 4 O2 complete reaction, during Arg hydroxylation, H4B acts as a one-electron donor and is then presumed to redox cycle, i.e. be reduced back to H4B, within NOS before further catalysis can proceed. Calmodulin-dependent reduction of a tetrahydrobiopterin radical, mechanism involving the NOS flavoprotein domain, reaction scheme, overview Rattus norvegicus 2 citrulline + 2 nitric oxide + 3 NADP+ + 4 H2O
-
 ?
1.14.13.39 additional information nitric-oxide synthases are catalytically self-sufficient flavo-heme enzymes that generate NO from L-arginine and display an utilization of their tetrahydrobiopterin cofactor, overview Rattus norvegicus ?
-
 ?
1.14.13.39 Nomega-hydroxy-L-arginine + NADPH + H+ + O2 second half reaction via intermediate Nomega-hydroxy-L-arginine Rattus norvegicus citrulline + nitric oxide + NADP+ + H2O
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.14.13.39 neuronal NOS
-
 Rattus norvegicus
1.14.13.39 NOS
-
 Rattus norvegicus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.14.13.39 7.5
-
 assay at Rattus norvegicus

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.13.39 Calmodulin dependent on Rattus norvegicus
1.14.13.39 FMNH2
-
 Rattus norvegicus
1.14.13.39 heme
-
 Rattus norvegicus
1.14.13.39 NADPH
-
 Rattus norvegicus
1.14.13.39 tetrahydrobiopterin
-
 Rattus norvegicus