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Literature summary extracted from

  • Keskiaho, K.; Kukkola, L.; Page, A.P.; Winter, A.D.; Vuoristo, J.; Sormunen, R.; Nissi, R.; Riihimaa, P.; Myllyharju, J.
    Characterization of a novel Caenorhabditis elegans prolyl 4-hydroxylase with a unique substrate specificity and restricted expression in the pharynx and excretory duct (2008), J. Biol. Chem., 283, 10679-10689.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.11.2 co-expression of PHY-4.1, product of alpha subunit-like gene Y43F8B.4, and beta-subunit protein-disulfide isomerase PDI2, in Sf9 cell Caenorhabditis elegans

Protein Variants

EC Number Protein Variants Comment Organism
1.14.11.2 additional information co-expression of PHY-4.1, product of alpha subunit-like gene Y43F8B.4, and beta-subunit protein-disulfide isomerase PDI2 results in active (PHY-4.1)2(PDI2)2 tetramers and in PHY-4.1/PDI2 dimers. Contrary to alpha subunits PHY-1, PHY-2, PHY-3, tetramers containing PHY-4.1 are able to hydroxylate poly(L-Pro) and other proline-rich peptides Caenorhabditis elegans

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.14.11.2 0.03
-
 2-oxoglutarate complex of recombinant subunits PHY-4.1 and PDI2 Caenorhabditis elegans
1.14.11.2 0.11
-
 poly(L-Pro) Mr 30000-40000 da, complex of recombinant subunits PHY-4.1 and PDI2 Caenorhabditis elegans
1.14.11.2 0.22
-
 poly(L-Pro) Mr 5000-10000 Da, complex of recombinant subunits PHY-4.1 and PDI2 Caenorhabditis elegans
1.14.11.2 0.25
-
 (L-Pro-L-Glu-L-Pro-L-Pro-L-Ala)5 L-proline complex of recombinant subunits PHY-4.1 and PDI2 Caenorhabditis elegans
1.14.11.2 2.5
-
 (L-Pro-L-Pro-Gly)10 L-proline complex of recombinant subunits PHY-4.1 and PDI2 Caenorhabditis elegans

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.14.11.2 Fe2+ complex of recombinant subunits PHY-4.1 and PDI2, Km value 0.01 mM Caenorhabditis elegans

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.14.11.2 90000
-
 PAGE Caenorhabditis elegans
1.14.11.2 180000
-
 PAGE Caenorhabditis elegans

Organism

EC Number Organism UniProt Comment Textmining
1.14.11.2 Caenorhabditis elegans
-
 PHY-4.1, product of alpha subunit-like gene Y43F8B.4
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
1.14.11.2 glycoprotein recombinant alpha subunit PHY4.1 is partly N-glycosylated Caenorhabditis elegans
1.14.11.2 proteolytic modification sequence contains a GP67 signal peptide Caenorhabditis elegans

Source Tissue

EC Number Source Tissue Comment Organism Textmining

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.11.2 (L-Pro-L-Glu-L-Pro-L-Pro-L-Ala)5 L-proline + 2-oxoglutarate + O2
-
 Caenorhabditis elegans (L-Pro-L-Glu-L-Pro-L-Pro-L-Ala)5 trans-4-hydroxy-L-proline + succinate + CO2
-
 ?
1.14.11.2 (L-Pro-L-Pro-Gly)10 L-proline + 2-oxoglutarate + O2 44.8% of the activity with (L-Pro-L-Glu-L-Pro-L-Pro-L-Ala)5 L-proline Caenorhabditis elegans (L-Pro-L-Pro-Gly)10 trans-4-hydroxy-L-proline + succinate + CO2
-
 ?
1.14.11.2 L-Ala-(L-Pro)4-Gly-L-Ile-L-Pro-Gly-L-Tyr-(L-Pro)2-L-Ala-(L-Pro)4-Gly-L-Val-Gly-(L-Pro)4-L-Gln-Gly L-proline + 2-oxoglutarate + O2
-
 Caenorhabditis elegans L-Ala-(L-Pro)4-Gly-L-Ile-L-Pro-Gly-L-Tyr-(L-Pro)2-L-Ala-(L-Pro)4-Gly-L-Val-Gly-(L-Pro)4-L-Gln-Gly trans-4-hydroxy-L-proline + succinate + CO2 peptide substrate representing proline-rich proteins expressed in the pharyngeal gland cells. 380% of the activity with (L-Pro-L-Glu-L-Pro-L-Pro-L-Ala)5 L-proline ?
1.14.11.2 L-Pro-L-Ile-(L-Pro)7-L-Leu-L-Pro-L-Gln-L-Asn-L-Leu-L-Ser-Gly-L-Ala-L-Pro L-proline + 2-oxoglutarate + O2
-
 Caenorhabditis elegans L-Pro-L-Ile-(L-Pro)7-L-Leu-L-Pro-L-Gln-L-Asn-L-Leu-L-Ser-Gly-L-Ala-L-Pro trans-4-hydroxy-L-proline + succinate + CO2 peptide substrate representing proline-rich proteins expressed in the pharyngeal gland cells. 610% of the activity with (L-Pro-L-Glu-L-Pro-L-Pro-L-Ala)5 L-proline ?
1.14.11.2 poly(L-Pro) + 2-oxoglutarate + O2 Mr 30000-40000 Da, 15% of the activity with (L-Pro-L-Glu-L-Pro-L-Pro-L-Ala)5 L-proline Caenorhabditis elegans poly(trans-4-hydroxy-L-proline) + succinate + CO2
-
 ?
1.14.11.2 poly(L-Pro) + 2-oxoglutarate + O2 Mr 5000-10000 Da, 5, 24% of the activity with (L-Pro-L-Glu-L-Pro-L-Pro-L-Ala)5 L-proline Caenorhabditis elegans poly(trans-4-hydroxy-L-proline) + succinate + CO2
-
 ?

Subunits

EC Number Subunits Comment Organism
1.14.11.2 dimer and tetramer, 1 * alpha subunit PHY4.1 + 1 * beta subunit PDI2, SDS-PAGE of recombinant protein Caenorhabditis elegans
1.14.11.2 tetramer and dimer, 2 * alpha subunit PHY4.1 + 2 * beta subunit PDI2, SDS-PAGE of recombinant protein Caenorhabditis elegans

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.11.2 ascorbate complex of recombinant subunits PHY-4.1 and PDI2, Km value 0.04 mM Caenorhabditis elegans