Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Broecker, M.J.; Virus, S.; Ganskow, S.; Heathcote, P.; Heinz, D.W.; Schubert, W.D.; Jahn, D.; Moser, J.
    ATP-driven reduction by dark-operative protochlorophyllide oxidoreductase from Chlorobium tepidum mechanistically resembles nitrogenase catalysis (2008), J. Biol. Chem., 283, 10559-10567.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.3.1.33 expressed in Escherichia coli BL21(DE3) Codon Plus RIL cells Chlorobaculum tepidum

Protein Variants

EC Number Protein Variants Comment Organism
1.3.1.33 C103S the BchN subunit variant shows 0.5% residual activity and is essentially inactive Chlorobaculum tepidum
1.3.1.33 C21S the BchN subunit variant shows 0.5% residual activity and is essentially inactive Chlorobaculum tepidum
1.3.1.33 C46S the BchN subunit variant shows 0.5% residual activity and is essentially inactive Chlorobaculum tepidum

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.3.1.33 cyanide
-
Chlorobaculum tepidum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.3.1.33 0.0061
-
protochlorophyllide in 100 mM HEPES-NaOH (pH 7.5), 2 mM ATP, 5 mM MgCl2 Chlorobaculum tepidum
1.3.1.33 0.0527
-
Dithionite in 100 mM HEPES-NaOH (pH 7.5), 2 mM ATP, 5 mM MgCl2 Chlorobaculum tepidum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.3.1.33 protochlorophyllide + reduced ferredoxin Chlorobaculum tepidum ferredoxin is the natural electron donor chlorophyllide + oxidized ferredoxin
-
?

Organism

EC Number Organism UniProt Comment Textmining
1.3.1.33 Chlorobaculum tepidum
-
strain ATCC 49652
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.3.1.33 glutathione-Sepharose column chromatography Chlorobaculum tepidum

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.3.1.33 0.00315
-
in 100 mM HEPES-NaOH (pH 7.5), 2 mM ATP, 5 mM MgCl2 Chlorobaculum tepidum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.3.1.33 protochlorophyllide + dithionite
-
Chlorobaculum tepidum chlorophyllide + SO2
-
?
1.3.1.33 protochlorophyllide + dithiothreitol
-
Chlorobaculum tepidum chlorophyllide + oxidized dithiothreitol
-
?
1.3.1.33 protochlorophyllide + reduced ferredoxin ferredoxin is the natural electron donor Chlorobaculum tepidum chlorophyllide + oxidized ferredoxin
-
?

Synonyms

EC Number Synonyms Comment Organism
1.3.1.33 dark-operative protochlorophyllide oxidoreductase
-
Chlorobaculum tepidum
1.3.1.33 DPOR
-
Chlorobaculum tepidum
1.3.1.33 light-independent (dark) Pchlide oxidoreductase
-
Chlorobaculum tepidum

Cofactor

EC Number Cofactor Comment Organism Structure
1.3.1.33 4Fe-4S center
-
Chlorobaculum tepidum
1.3.1.33 ATP
-
Chlorobaculum tepidum