EC Number | Cloned (Comment) | Organism |
---|---|---|
2.3.1.26 | all constructs, full-length, chimeras and mutants are cloned into the pCDNA3 vector, AC29 cells are transfected | Chlorocebus aethiops |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
2.3.1.26 | A1:1-123 | chimeric protein A1(1-123)-A2(105-526) | Chlorocebus aethiops |
2.3.1.26 | A1:1-385 | chimeric protein A1(1-385)-A2(364-440)-A1(463-550) | Chlorocebus aethiops |
2.3.1.26 | A1:1-71 | chimeric protein A1(1-71)-A2(70-562) | Chlorocebus aethiops |
2.3.1.26 | A2:1-227 | chimeric protein A2(1-227)-A1(248-489)-A2(468-526) | Chlorocebus aethiops |
2.3.1.26 | A2:1-249 | chimeric protein A2(1-249)-A1(270-362)-A2(341-526) | Chlorocebus aethiops |
2.3.1.26 | A2:1-363 | chimeric protein A2(1-363)-A1(386-462)-A2(441-526) | Chlorocebus aethiops |
2.3.1.26 | A2:1-428 | chimeric protein A2(1-428)-A1(444-550) | Chlorocebus aethiops |
2.3.1.26 | A2:1-440 | chimeric protein A2(1-440)-A1(463-502)-A2(480-526) | Chlorocebus aethiops |
2.3.1.26 | A2:1-479 | chimeric protein A2(1-479)-A1(502-550) | Chlorocebus aethiops |
2.3.1.26 | A2C497S | A2 mutant | Chlorocebus aethiops |
2.3.1.26 | A2I491V | A2 mutant | Chlorocebus aethiops |
2.3.1.26 | A2L480M | A2 mutant | Chlorocebus aethiops |
2.3.1.26 | A2L495F | A2 mutant | Chlorocebus aethiops |
2.3.1.26 | A2M484S | A2 mutant | Chlorocebus aethiops |
2.3.1.26 | A2Q489N | A2 mutant | Chlorocebus aethiops |
2.3.1.26 | A2Q492L | A2 mutant | Chlorocebus aethiops |
2.3.1.26 | A2R504Q | A2 mutant | Chlorocebus aethiops |
2.3.1.26 | A2S494C | A2 mutant | Chlorocebus aethiops |
2.3.1.26 | A2V493L | A2 mutant | Chlorocebus aethiops |
2.3.1.26 | Ch1 | chimeric protein A2(1-428)-A1(451-462)-A2(441-526) | Chlorocebus aethiops |
2.3.1.26 | CT504 | A2(1-504) | Chlorocebus aethiops |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
2.3.1.26 | pyripyropene A | - |
Chlorocebus aethiops |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.3.1.26 | 0.00000266 | - |
cis-9-octadecenoyl-CoA | pyripyropene A-insensitive chimera A2:1-479 | Chlorocebus aethiops | |
2.3.1.26 | 0.00000333 | - |
cis-9-octadecenoyl-CoA | pyripyropene A-insensitive chimera A2:1-428 | Chlorocebus aethiops | |
2.3.1.26 | 0.00000553 | - |
cis-9-octadecenoyl-CoA | wild typeACAT2 | Chlorocebus aethiops | |
2.3.1.26 | 0.00000587 | - |
cis-9-octadecenoyl-CoA | pyripyropene A-insensitive mutant A2S494C | Chlorocebus aethiops | |
2.3.1.26 | 0.000008094 | - |
cis-9-octadecenoyl-CoA | pyripyropene A-insensitive mutant A2V493L | Chlorocebus aethiops | |
2.3.1.26 | 0.00001218 | - |
cis-9-octadecenoyl-CoA | pyripyropene A-insensitive mutant A2Q492L | Chlorocebus aethiops |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.3.1.26 | acyl-CoA + cholesterol | Chlorocebus aethiops | - |
CoA + cholesterol ester | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.3.1.26 | Chlorocebus aethiops | - |
African green monkey | - |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.3.1.26 | microsomes from AC29 cells are prepared | Chlorocebus aethiops |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.3.1.26 | acyl-CoA + cholesterol | - |
Chlorocebus aethiops | CoA + cholesterol ester | - |
? | |
2.3.1.26 | oleoyl-CoA + cholesterol | - |
Chlorocebus aethiops | CoA + cholesteryl oleate | - |
? | |
2.3.1.26 | oleoyl-CoA + cholesterol | activity assay | Chlorocebus aethiops | ? | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
2.3.1.26 | A2 | - |
Chlorocebus aethiops |
2.3.1.26 | ACAT2 | - |
Chlorocebus aethiops |
2.3.1.26 | acyl-CoA:cholesterol acyltransferase 2 | - |
Chlorocebus aethiops |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.3.1.26 | 37 | - |
microsomal ACAT assay | Chlorocebus aethiops |