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Literature summary extracted from

  • Soto, A.; Carman, G.M.
    Regulation of the Saccharomyces cerevisiae CKI1-encoded choline kinase by zinc depletion (2008), J. Biol. Chem., 283, 10079-10088.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.7.1.32 Zinc intacellular zinc depletion resulted in a concentration-dependent induction of CKI1 expression Saccharomyces cerevisiae

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.1.32 plasmid maintenance and amplification are performed in Escherichia coli strain DH5 alpha Saccharomyces cerevisiae

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.1.32 choline + ATP Saccharomyces cerevisiae The induction of CKI1 expression in zinc-depleted cells translated into increased choline kinase activity in vitro and in vivo and in an increase in phosphatidylcholine synthesis via the Kennedy pathway phosphocholine + ADP
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Organism

EC Number Organism UniProt Comment Textmining
2.7.1.32 Saccharomyces cerevisiae
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-
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Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.1.32 yeast form strains W303-1A,DY1457, ZHY6, ZHY3, SH304, SH303, SH307 Saccharomyces cerevisiae
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Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.1.32 additional information
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 The specific activity of choline kinase in wild type zinc-depleted cells is 2.7fold greater when compared with the activity from cells grown with 1.5 microM ZnSO4. Choline kinase activity is measured in a reaction mixture that contains 67 mM glycine-NaOH, 5 mM (methyl-14C)choline, 5 mM ATP, 10mM MgSO4 and 1.3 mM dithiothreitol. The radiolabeled product phosphocholine, is separated from the substrate by precipitation of choline as a reineckate salt. Saccharomyces cerevisiae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.32 choline + ATP The induction of CKI1 expression in zinc-depleted cells translated into increased choline kinase activity in vitro and in vivo and in an increase in phosphatidylcholine synthesis via the Kennedy pathway Saccharomyces cerevisiae phosphocholine + ADP
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 ?

Synonyms

EC Number Synonyms Comment Organism
2.7.1.32 choline kinase
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 Saccharomyces cerevisiae
2.7.1.32 CKI1
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 Saccharomyces cerevisiae
2.7.1.32 CKI1-encoded choline kinase
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 Saccharomyces cerevisiae

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.1.32 9.5
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 assay at Saccharomyces cerevisiae

Cofactor

EC Number Cofactor Comment Organism Structure
2.7.1.32 ATP
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 Saccharomyces cerevisiae