Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Tian, E.; ten Hagen, K.G.
    A UDP-GalNAc:polypeptide N-acetylgalactosaminyltransferase is required for epithelial tube formation (2007), J. Biol. Chem., 282, 606-614.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.1.41 PGANT35A in pUAST plasmid for trachea-specific, Gal4-iduceable expression in transgenic flies under control of breathless promoter Drosophila melanogaster

Protein Variants

EC Number Protein Variants Comment Organism
2.4.1.41 additional information development of pgant35A mutant germ line clones arrests to 88% during embryogenesis, does not reach pupation Drosophila melanogaster
2.4.1.41 additional information pgant35A mutant phenotype less severe and 7% rescue of lethality upon trachea-specific expression of PGANT35A wild-type Drosophila melanogaster
2.4.1.41 additional information severity of phenotypes of transgenic flies correlates with molecular and biochemical characterization of the severity of the mutant allele Drosophila melanogaster
2.4.1.41 pgant35A-3775 transgenic fly: heterozygous with pgant35A-SF32 or pgant35A-HG8 allele, germ line clone: heterozygous with pgant35A-HG8 or pgant35A-SF32 allele, nonsense mutation results in translational stop and loss of enzymatic activity Drosophila melanogaster
2.4.1.41 pgant35A-HG8 transgenic fly: heterozygous with pgant35A-SF32 or pgant35A-3775 phenotype, germ line clone: homozygous or with pgant35A-3775 allele , nonsense mutation results in translational stop and loss of enzymatic activity Drosophila melanogaster
2.4.1.41 pgant35A-SF32 transgenic fly: heterozygous with pgant35A-3775 or pgant35A-HG8 phenotype, germ line clone: homozygous or with pgant35A-3775 allele, missense mutation: Arg to Trp, results in diminished activity Drosophila melanogaster

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.1.41 UDP-N-acetyl-D-galactosamine + polypeptide Drosophila melanogaster mucin-type O-linked glycosylation of serine or threonine residues UDP + N-acetyl-D-galactosaminyl-polypeptide
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.41 Drosophila melanogaster Q8MVS5 PGANT35A; PGANT35A is an orthologue of mammalian ppGalNTase -T11
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.4.1.41 embryonic tracheal system dorsal longitudinal tracheal trunks and tracheal transverse connectives at embryonic stages 15-17, loss of PGANT35A activity leads to tracheal defects at embryonic stages 15-17, altered intracellular trafficking, reduction in apical and luminal epithelial marker components, alterations in cell shape, polarity and zona adherens formation, mislocation and disfunction of septate junctions, disruption of paracellular diffusion barrier formation Drosophila melanogaster
-
2.4.1.41 hindgut loss of PGANT35A activity does not alter morphology or staining patterns of epithelial marker components Drosophila melanogaster
-
2.4.1.41 salivary gland loss of PGANT35A activity does not alter morphology or staining patterns of epithelial marker components Drosophila melanogaster
-
2.4.1.41 trachea loss of PGANT35A activity leads to diminished O-glycosylation in epithelial cells of tracheal tube as well as reduced amount of GalNAc alpha-S/T glycans at the apical cell surface Drosophila melanogaster
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.41 UDP-alpha-GalNAc + polypeptide
-
 Drosophila melanogaster GalNAc-alpha-polypeptide + UDP major mucin-type O-glycan in embryonic tracheal system along the apical region and luman of the tracheal tubes O-glycan, detectable by lectins Jacalin, soybean agglutinin and Vicia villosa agglutinin ?
2.4.1.41 UDP-N-acetyl-D-galactosamine + polypeptide mucin-type O-linked glycosylation of serine or threonine residues Drosophila melanogaster UDP + N-acetyl-D-galactosaminyl-polypeptide
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.4.1.41 PGANT
-
 Drosophila melanogaster
2.4.1.41 ppGalNAcT
-
 Drosophila melanogaster
2.4.1.41 ppGaNTase
-
 Drosophila melanogaster
2.4.1.41 UDP-GalNAc:polypeptide N-acetylgalactosaminyltransferase
-
 Drosophila melanogaster