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Literature summary extracted from
- Inhibition of human dimethylarginine dimethylaminohydrolase-1 by S-nitroso-L-homocysteine and hydrogen peroxide. Analysis, quantification, and implications for hyperhomocysteinemia (2007), J. Biol. Chem., 282, 34684-34692.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 3.5.3.18 | expressed in Escherichia coli BL21 (DE3) cells | Homo sapiens |
Protein Variants
| EC Number | Protein Variants | Comment | Organism |
|---|---|---|---|
| 3.5.3.18 | C274A | no detectable activity | Homo sapiens |
| 3.5.3.18 | C275A | retains a kcat value about half of wild type protein | Homo sapiens |
| 3.5.3.18 | H173A | no detectable activity | Homo sapiens |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.5.3.18 | additional information | insensitive to hydrogen peroxide | Homo sapiens | |
| 3.5.3.18 | S-nitroso-L-homocysteine | - |
Homo sapiens |  |
| 3.5.3.18 | Zn2+ | - |
Homo sapiens | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.5.3.18 | 33440 | - |
electrospray ionization mass spectrometry | Homo sapiens |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.5.3.18 | Homo sapiens | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.5.3.18 | Ni-NTA affinity resin chromatography and phenyl-Sepharose column chromatography | Homo sapiens |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.5.3.18 | additional information | L-arginine is no substrate | Homo sapiens | ? | - |
? | |
| 3.5.3.18 | NG,NG-dimethyl-L-arginine + H2O | - |
Homo sapiens | dimethylamine + L-citrulline | - |
? | |
| 3.5.3.18 | NG-monomethyl-L-arginine + H2O | - |
Homo sapiens | citrulline + methylamine | - |
? | |
| 3.5.3.18 | S-methyl-L-thiocitrulline + H2O | - |
Homo sapiens | methanethiol + L-citrulline | - |
? | |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 3.5.3.18 | DDAH-1 | isozyme | Homo sapiens |
| 3.5.3.18 | dimethylarginine dimethylaminohydrolase | - |
Homo sapiens |
pH Stability
| EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.5.3.18 | 10 | - |
activity decreases at pH values above 10 | Homo sapiens |
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Release 2023.1 (January 2023)
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