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Literature summary extracted from

  • Waditee, R.; Bhuiyan, N.H.; Hirata, E.; Hibino, T.; Tanaka, Y.; Shikata, M.; Takabe, T.
    Metabolic engineering for betaine accumulation in microbes and plants (2007), J. Biol. Chem., 282, 34185-34193.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.1.1.95 CaCl2
-
 Aphanothece halophytica
1.1.1.95 KCl
-
 Aphanothece halophytica
1.1.1.95 MgCl2
-
 Aphanothece halophytica
1.1.1.95 NaCl PGDH activity increase about 1.5-2.3fold upon the increase of salinity from 0.5 to 2.5 M NaCl Aphanothece halophytica

Application

EC Number Application Comment Organism
1.1.1.95 molecular biology results demonstrate that PGDH enhances the levels of betaine by providing the precursor serine for both choline oxidation and glycine methylation pathways Aphanothece halophytica

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.95 expressed in Escherichia coli and Arabidopsis thaliana Aphanothece halophytica

Protein Variants

EC Number Protein Variants Comment Organism
1.1.1.95 additional information expression of the Aphanothece PGDH gene in Escherichia coli causes an increase in levels of betaine as well as glycine and serine. Expression of the Aphanothece PGDH gene in Arabidopsis thaliana, in which the betaine synthetic pathway is introduced via glycine methylation, further increases betaine levels and improves the stress tolerance Aphanothece halophytica

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.95 0.15
-
 3-phosphohydroxypyruvate
-
 Aphanothece halophytica
1.1.1.95 0.9
-
 3-phospho-D-glycerate
-
 Aphanothece halophytica

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.95 56410
-
 deduced from cDNA Aphanothece halophytica
1.1.1.95 57000
-
 SDS-PAGE Aphanothece halophytica

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.95 Aphanothece halophytica
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.95 using ammonium sulphate fractionation and ion exchange chromatography Aphanothece halophytica

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.95 3-phospho-D-glycerate + NAD+
-
 Aphanothece halophytica 3-phosphohydroxypyruvate + NADH + H+
-
 r

Subunits

EC Number Subunits Comment Organism
1.1.1.95 tetramer gel filtration Aphanothece halophytica

Synonyms

EC Number Synonyms Comment Organism
1.1.1.95 3-phosphoglycerate dehydrogenase
-
 Aphanothece halophytica
1.1.1.95 ApPGDH
-
 Aphanothece halophytica

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.1.95 30
-
 assay at Aphanothece halophytica

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.1.1.95 additional information
-
 activity remains stable after incubation at 40°C for 7 min, but decreases to 50% when the enzyme is incubated at 50°C for 7 min Aphanothece halophytica

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.1.1.95 7.5
-
 3-phosphohydroxypyruvate reduction Aphanothece halophytica
1.1.1.95 9
-
 3-phospho-D-glycerate oxidation Aphanothece halophytica