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Literature summary extracted from

  • Donato, H.; Krupenko, N.I.; Tsybovsky, Y.; Krupenko, S.A.
    10-formyltetrahydrofolate dehydrogenase requires a 4-phosphopantetheine prosthetic group for catalysis (2007), J. Biol. Chem., 282, 34159-34166.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.5.1.6 expressed in Escherichia coli and Sf9 insect cells Rattus norvegicus

Protein Variants

EC Number Protein Variants Comment Organism
1.5.1.6 S354A no dehydrogenase activity Rattus norvegicus

Organism

EC Number Organism UniProt Comment Textmining
1.5.1.6 Rattus norvegicus
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
1.5.1.6 additional information FDH requires posttranslational modification to become a functional enzyme (4'-phosphopantetheine prosthetic group) Rattus norvegicus

Purification (Commentary)

EC Number Purification (Comment) Organism
1.5.1.6 5-fTHFSepharose column chromatography, Sephacryl S-300 gel filtration, and MonoQ column chromatography Rattus norvegicus

Reaction

EC Number Reaction Comment Organism Reaction ID
1.5.1.6 10-formyltetrahydrofolate + NADP+ + H2O = tetrahydrofolate + CO2 + NADPH + H+ FDH catalyzes the hydrolytic cleavage of the formyl group in the N-terminal catalytic domain, followed by NADP+-dependent oxidation of the formyl group toCO2 in the C-terminal aldehyde dehydrogenase domain Rattus norvegicus
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.5.1.6 10-formyltetrahydrofolate + NADP+ + H2O
-
 Rattus norvegicus tetrahydrofolate + CO2 + NADPH + H+
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.5.1.6 10-formyltetrahydrofolate dehydrogenase
-
 Rattus norvegicus
1.5.1.6 ALDH1L1
-
 Rattus norvegicus
1.5.1.6 FDH the enzyme has also hydrolase and aldehyde dehydrogenase activities Rattus norvegicus

Cofactor

EC Number Cofactor Comment Organism Structure
1.5.1.6 NADP+ dependent Rattus norvegicus