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Literature summary extracted from

  • Cooper, W.C.; Jin, Y.; Penning, T.M.
    Elucidation of a complete kinetic mechanism for a mammalian hydroxysteroid dehydrogenase (HSD) and identification of all enzyme forms on the reaction coordinate: the example of rat liver 3alpha-HSD (AKR1C9) (2007), J. Biol. Chem., 282, 33484-33493.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.50 additional information
-
 additional information steady-state, transient state kinetics, and kinetic isotope effects, kinetic analysis and mechanism, ordered bi-bi mechanism, detailed overview Rattus norvegicus
1.1.1.50 0.00065
-
 5alpha-androstane-3,17-dione pH 7.0, 25, reduction reaction Rattus norvegicus
1.1.1.50 0.0041
-
 androsterone pH 7.0, 25, oxidation reaction Rattus norvegicus
1.1.1.213 additional information
-
 additional information steady-state, transient state kinetics, and kinetic isotope effects, kinetic analysis and mechanism, ordered bi-bi mechanism, detailed overview Rattus norvegicus
1.1.1.213 0.00065
-
 5alpha-androstane-3,17-dione pH 7.0, 25, reduction reaction Rattus norvegicus
1.1.1.213 0.0041
-
 androsterone pH 7.0, 25, oxidation reaction Rattus norvegicus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.50 androsterone + NADP+ Rattus norvegicus
-
 5alpha-androstane-3,17-dione + NADPH
-
 r
1.1.1.213 androsterone + NADP+ Rattus norvegicus
-
 5alpha-androstane-3,17-dione + NADPH
-
 r

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.50 Rattus norvegicus
-
-
-
1.1.1.213 Rattus norvegicus
-
-
-

Reaction

EC Number Reaction Comment Organism Reaction ID
1.1.1.50 a 3alpha-hydroxysteroid + NAD(P)+ = a 3-oxosteroid + NAD(P)H + H+ bi bi ordered mechanism, kinetic analysis, overview Rattus norvegicus
a
1.1.1.213 a 3alpha-hydroxysteroid + NAD(P)+ = a 3-oxosteroid + NAD(P)H + H+ bi bi ordered mechanism, kinetic analysis, overview Rattus norvegicus
a

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.1.50 liver
-
 Rattus norvegicus
-
1.1.1.213 liver
-
 Rattus norvegicus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.50 androsterone + NADP+
-
 Rattus norvegicus 5alpha-androstane-3,17-dione + NADPH
-
 r
1.1.1.213 androsterone + NADP+
-
 Rattus norvegicus 5alpha-androstane-3,17-dione + NADPH
-
 r

Synonyms

EC Number Synonyms Comment Organism
1.1.1.50 3alpha-HSD
-
 Rattus norvegicus
1.1.1.50 AKR1C9
-
 Rattus norvegicus
1.1.1.50 More cf. EC 1.1.1.213, the enzyme is a member of the aldo-keto reductase superfamily Rattus norvegicus
1.1.1.213 3alpha-HSD
-
 Rattus norvegicus
1.1.1.213 AKR1C9
-
 Rattus norvegicus
1.1.1.213 More cf. EC 1.1.1.50, the enzyme is a member of the aldo-keto reductase superfamily Rattus norvegicus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.1.50 25
-
 assay at Rattus norvegicus
1.1.1.213 25
-
 assay at Rattus norvegicus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.1.1.50 0.42
-
 5alpha-androstane-3,17-dione pH 7.0, 25, reduction reaction Rattus norvegicus
1.1.1.50 0.82
-
 androsterone pH 7.0, 25, oxidation reaction Rattus norvegicus
1.1.1.213 0.42
-
 5alpha-androstane-3,17-dione pH 7.0, 25, reduction reaction Rattus norvegicus
1.1.1.213 0.82
-
 androsterone pH 7.0, 25, oxidation reaction Rattus norvegicus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.50 7
-
 assay at Rattus norvegicus
1.1.1.213 7
-
 assay at Rattus norvegicus

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.50 NADP+ loose association of the NADP(H) is followed by two conformational changes, which increases cofactor affinity by 86fold Rattus norvegicus
1.1.1.50 NADPH loose association of the NADP(H) is followed by two conformational changes, which increases cofactor affinity by 86fold Rattus norvegicus
1.1.1.213 NADP+ loose association of the NADP(H) is followed by two conformational changes, which increases cofactor affinity by 86fold Rattus norvegicus
1.1.1.213 NADPH loose association of the NADP(H) is followed by two conformational changes, which increases cofactor affinity by 86fold Rattus norvegicus