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Literature summary extracted from

  • Gao, Y.T.; Roman, L.J.; Martasek, P.; Panda, S.P.; Ishimura, Y.; Masters, B.S.
    Oxygen metabolism by endothelial nitric-oxide synthase (2007), J. Biol. Chem., 282, 28557-28565.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.14.13.39 Calmodulin activates O2 consumption of eNOS Rattus norvegicus
1.14.13.39 L-arginine L-arginine strongly stimulates oxygen consumption of eNOS and inhibits that of nNOS, nonhydrolyzable L-arginine analogues are not stimulatory Rattus norvegicus

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.13.39 expression of eNOS in Escherichia coli Rattus norvegicus

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.14.13.39 5,6,7,8-tetrahydrobiopterin quenches the uncoupled reactions and results in much less reactive oxygen species formation, whereas the presence of redox-incompetent 7,8-dihydrobiopterin demonstrates little quenching effect Rattus norvegicus
1.14.13.39 L-arginine L-arginine strongly stimulates oxygen consumption of eNOS and inhibits that of nNOS Rattus norvegicus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.14.13.39 Ca2+ required by eNOS, activates Rattus norvegicus
1.14.13.39 Fe2+ a cytochrome P450 enzyme Rattus norvegicus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.13.39 2 L-arginine + 3 NADPH + 3 H+ + 4 O2 Rattus norvegicus
-
 2 citrulline + 2 nitric oxide + 3 NADP+ + 4 H2O
-
 ?
1.14.13.39 additional information Rattus norvegicus eNOS uncoupling is known to be controlled by substrate/cofactor availability, and the uncoupled reactions play important roles under various physiological/pathological conditions, such as atherosclerosis and septic shock ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.14.13.39 Rattus norvegicus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.14.13.39 recombinant eNOS from Escherichia coli by 2',5'-ADP affinity chromatography Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.13.39 2 L-arginine + 2 NADPH + 2 H+ + 2 O2 first half reaction via intermediate Nomega-hydroxy-L-arginine Rattus norvegicus 2 Nomega-hydroxy-L-arginine + 2 NADP+ + 2 H2O
-
 ?
1.14.13.39 2 L-arginine + 3 NADPH + 3 H+ + 4 O2
-
 Rattus norvegicus 2 citrulline + 2 nitric oxide + 3 NADP+ + 4 H2O
-
 ?
1.14.13.39 2 L-arginine + 3 NADPH + 3 H+ + 4 O2 complete reaction, oxygen stoichiometry, effects of substrate/cofactor binding on the endothelial NOS isoform, eNOS, overview Rattus norvegicus 2 citrulline + 2 nitric oxide + 3 NADP+ + 4 H2O
-
 ?
1.14.13.39 additional information eNOS uncoupling is known to be controlled by substrate/cofactor availability, and the uncoupled reactions play important roles under various physiological/pathological conditions, such as atherosclerosis and septic shock Rattus norvegicus ?
-
 ?
1.14.13.39 Nomega-hydroxy-L-arginine + NADPH + H+ + O2 second half reaction via intermediate Nomega-hydroxy-L-arginine Rattus norvegicus citrulline + nitric oxide + NADP+ + H2O
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.14.13.39 endothelial nitric-oxide synthase
-
 Rattus norvegicus
1.14.13.39 eNOS
-
 Rattus norvegicus
1.14.13.39 neuronal nitric-oxide synthase
-
 Rattus norvegicus
1.14.13.39 nNOS
-
 Rattus norvegicus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.14.13.39 24
-
 assay at Rattus norvegicus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.14.13.39 7.4
-
 assay at Rattus norvegicus

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.13.39 cytochrome c
-
 Rattus norvegicus
1.14.13.39 NADPH
-
 Rattus norvegicus