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Literature summary extracted from

  • Wakita, M.; Masuda, S.; Motohashi, K.; Hisabori, T.; Ohta, H.; Takamiya, K.
    The significance of type II and PrxQ peroxiredoxins for antioxidative stress response in the purple bacterium Rhodobacter sphaeroides (2007), J. Biol. Chem., 282, 27792-27801.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.11.1.24 chitin-tagged protein is expressed in Escherichia coli BL21(DE3) cells Cereibacter sphaeroides

Protein Variants

EC Number Protein Variants Comment Organism
1.11.1.24 C44S inactive Cereibacter sphaeroides
1.11.1.24 C80S reduced activity Cereibacter sphaeroides

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.11.1.24 0.00475
-
 H2O2 PrxT, in 50 mM HEPES/NaOH, pH 7.3, at 25°C Cereibacter sphaeroides
1.11.1.24 0.00665
-
 cumene hydroperoxide PrxT, in 50 mM HEPES/NaOH, pH 7.3, at 25°C Cereibacter sphaeroides
1.11.1.24 0.00848
-
 cumene hydroperoxide PrxQ, in 50 mM HEPES/NaOH, pH 7.3, at 25°C Cereibacter sphaeroides
1.11.1.24 0.00856
-
 H2O2 PrxQ, in 50 mM HEPES/NaOH, pH 7.3, at 25°C Cereibacter sphaeroides

Organism

EC Number Organism UniProt Comment Textmining
1.11.1.24 Cereibacter sphaeroides
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.11.1.24 with chitin beads Cereibacter sphaeroides

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.11.1.24 cumene hydroperoxide + reduced thioredoxin
-
 Cereibacter sphaeroides 2-phenylpropan-2-ol + oxidized thioredoxin + H2O
-
 ?
1.11.1.24 H2O2 + reduced thioredoxin
-
 Cereibacter sphaeroides H2O + oxidized thioredoxin
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.11.1.24 PrxQ
-
 Cereibacter sphaeroides
1.11.1.24 PrxT
-
 Cereibacter sphaeroides
1.11.1.24 type II peroxiredoxin
-
 Cereibacter sphaeroides

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.11.1.24 0.274
-
 cumene hydroperoxide PrxT, in 50 mM HEPES/NaOH, pH 7.3, at 25°C Cereibacter sphaeroides
1.11.1.24 0.281
-
 cumene hydroperoxide PrxQ, in 50 mM HEPES/NaOH, pH 7.3, at 25°C Cereibacter sphaeroides
1.11.1.24 0.285
-
 H2O2 PrxQ, in 50 mM HEPES/NaOH, pH 7.3, at 25°C Cereibacter sphaeroides
1.11.1.24 0.285
-
 H2O2 PrxT, in 50 mM HEPES/NaOH, pH 7.3, at 25°C Cereibacter sphaeroides

Cofactor

EC Number Cofactor Comment Organism Structure
1.11.1.24 thioredoxin dependent Cereibacter sphaeroides