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Literature summary extracted from

  • Bergfeld, A.K.; Claus, H.; Vogel, U.; Muehlenhoff, M.
    Biochemical characterization of the polysialic acid-specific O-acetyltransferase NeuO of Escherichia coli K1 (2007), J. Biol. Chem., 282, 22217-22227.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.1.136 expressed in Escherichia coli as a C-terminal hexahistidine-tagged fusion protein Escherichia coli

Protein Variants

EC Number Protein Variants Comment Organism
2.3.1.136 H119A complete loss of enzymatic activity without affecting the oligomerization state Escherichia coli
2.3.1.136 NeuO + 12 hepta-nucleotide repeats a set of NeuO alleles with N-terminal 0, 12, 24, and 36 hepta-nucleotide repeats are generated: protein yield: 3.7 mg/L, Km (mM): 0.22 (acetyl-CoA), 1.1 (colomnic acid), kcat (1/sec): 0.56 (acetyl-CoA), 1.85 (colomnic acid) Escherichia coli
2.3.1.136 NeuO + 24 hepta-nucleotide repeats a set of NeuO alleles with N-terminal 0, 12, 24, and 36 hepta-nucleotide repeats are generated: protein yield: 5.0 mg/L, Km (mM): 0.27 (acetyl-CoA), 1.0 (colomnic acid), kcat (1/sec): 1.07 (acetyl-CoA), 2.57 (colomnic acid) Escherichia coli
2.3.1.136 NeuO + 36 hepta-nucleotide repeats a set of NeuO alleles with N-terminal 0, 12, 24, and 36 hepta-nucleotide repeats are generated: protein yield: 5.0 mg/L, Km (mM): 0.22 (acetyl-CoA), 0.22 (propionyl-CoA) 1.6 (colomnic acid), kcat (1/sec): 1.16 (acetyl-CoA), 0.09 (propionyl-CoA), 3.84 (colomnic acid) Escherichia coli
2.3.1.136 W143A complete loss of enzymatic activity without affecting the oligomerization state Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.1.136 0.22
-
 acetyl-CoA mutant NeuO + 12 hepta-nucleotide repeats Escherichia coli
2.3.1.136 0.22
-
 acetyl-CoA mutant NeuO + 36 hepta-nucleotide repeats Escherichia coli
2.3.1.136 0.22
-
 propionyl-CoA mutant NeuO + 36 hepta-nucleotide repeats Escherichia coli
2.3.1.136 0.27
-
 acetyl-CoA mutant NeuO + 24 hepta-nucleotide repeats Escherichia coli
2.3.1.136 0.4
-
 acetyl-CoA wild-type NeuO Escherichia coli
2.3.1.136 1
-
 colominic acid mutant NeuO + 24 hepta-nucleotide repeats Escherichia coli
2.3.1.136 1.06
-
 colominic acid mutant NeuO + 36 hepta-nucleotide repeats Escherichia coli
2.3.1.136 1.1
-
 colominic acid mutant NeuO + 12 hepta-nucleotide repeats Escherichia coli
2.3.1.136 1.17
-
 colominic acid wild-type NeuO Escherichia coli

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.3.1.136 26800
-
 SDS-PAGE Escherichia coli

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.136 Escherichia coli
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.1.136 using Ni2+-affinity chromatography Escherichia coli

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.3.1.136 additional information
-
 enzymatic activity increases linearly with increasing numbers of repeats Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.136 acetyl-CoA + (-8-Neu5Ac-alpha2)2
-
 Escherichia coli CoA + ?
-
 ?
2.3.1.136 acetyl-CoA + (-8-Neu5Ac-alpha2)3
-
 Escherichia coli CoA + ?
-
 ?
2.3.1.136 acetyl-CoA + (-8-Neu5Ac-alpha2)4
-
 Escherichia coli CoA + ?
-
 ?
2.3.1.136 acetyl-CoA + (-8-Neu5Ac-alpha2)5
-
 Escherichia coli CoA + ?
-
 ?
2.3.1.136 acetyl-CoA + CMP-Neu5Ac
-
 Escherichia coli CoA + ?
-
 ?
2.3.1.136 acetyl-CoA + colominic acid
-
 Escherichia coli CoA + O-(acetyl)-colominic acid
-
 ?
2.3.1.136 acetyl-CoA + Neu5Ac
-
 Escherichia coli CoA + O-(acetyl)-Neu5Ac
-
 ?
2.3.1.136 additional information enzyme shows a high substrate specificity toward polysialic acid with more than 14 residues Escherichia coli ?
-
 ?
2.3.1.136 propionyl-CoA + colominic acid
-
 Escherichia coli CoA + O-(propionyl)-colominic acid
-
 ?

Subunits

EC Number Subunits Comment Organism
2.3.1.136 hexamer gel filtration Escherichia coli

Synonyms

EC Number Synonyms Comment Organism
2.3.1.136 NeuO
-
 Escherichia coli
2.3.1.136 polysialic acid-specific O-acetyltransferase
-
 Escherichia coli

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.3.1.136 25
-
 assay at Escherichia coli

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.3.1.136 0.09
-
 propionyl-CoA mutant NeuO + 36 hepta-nucleotide repeats Escherichia coli
2.3.1.136 0.51
-
 acetyl-CoA wild-type NeuO Escherichia coli
2.3.1.136 0.56
-
 acetyl-CoA mutant NeuO + 12 hepta-nucleotide repeats Escherichia coli
2.3.1.136 1.07
-
 acetyl-CoA mutant NeuO + 24 hepta-nucleotide repeats Escherichia coli
2.3.1.136 1.16
-
 acetyl-CoA mutant NeuO + 36 hepta-nucleotide repeats Escherichia coli
2.3.1.136 1.85
-
 colominic acid mutant NeuO + 12 hepta-nucleotide repeats Escherichia coli
2.3.1.136 1.99
-
 colominic acid wild-type NeuO Escherichia coli
2.3.1.136 2.57
-
 colominic acid mutant NeuO + 24 hepta-nucleotide repeats Escherichia coli
2.3.1.136 3.84
-
 colominic acid mutant NeuO + 36 hepta-nucleotide repeats Escherichia coli

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.3.1.136 7.5
-
 assay at Escherichia coli