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Literature summary extracted from

  • Chartier, F.J.; Couture, M.
    Substrate-specific interactions with the heme-bound oxygen molecule of nitric-oxide synthase (2007), J. Biol. Chem., 282, 20877-20886.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.14.13.39 tetrahydrobiopterin
-
 Staphylococcus aureus
1.14.13.39 tetrahydrobiopterin
-
 Bacillus subtilis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.14.13.39 additional information
-
 additional information kinetics and kinetic modelling, stopped-flow measurements Staphylococcus aureus
1.14.13.39 additional information
-
 additional information kinetics and kinetic modelling, stopped-flow measurements Bacillus subtilis

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.14.13.39 Fe2+ a heme enzyme, frequencies of electron transfer, overview Staphylococcus aureus
1.14.13.39 Fe2+ a heme enzyme, frequencies of electron transfer, overview Bacillus subtilis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.13.39 2 L-arginine + 3 NADPH + 3 H+ + 4 O2 Staphylococcus aureus
-
 2 citrulline + 2 nitric oxide + 3 NADP+ + 4 H2O
-
 ?
1.14.13.39 2 L-arginine + 3 NADPH + 3 H+ + 4 O2 Bacillus subtilis
-
 2 citrulline + 2 nitric oxide + 3 NADP+ + 4 H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.14.13.39 Bacillus subtilis
-
-
-
1.14.13.39 Staphylococcus aureus
-
-
-

Reaction

EC Number Reaction Comment Organism Reaction ID
1.14.13.39 2 L-arginine + 3 NADPH + 3 H+ + 4 O2 = 2 L-citrulline + 2 nitric oxide + 3 NADP+ + 4 H2O reaction and kinetic mechanism Staphylococcus aureus
a
1.14.13.39 2 L-arginine + 3 NADPH + 3 H+ + 4 O2 = 2 L-citrulline + 2 nitric oxide + 3 NADP+ + 4 H2O reaction and kinetic mechanism Bacillus subtilis
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.13.39 2 L-arginine + 2 NADPH + 2 H+ + 2 O2 first half reaction via intermediate Nomega-hydroxy-L-arginine Staphylococcus aureus 2 Nomega-hydroxy-L-arginine + 2 NADP+ + 2 H2O
-
 ?
1.14.13.39 2 L-arginine + 2 NADPH + 2 H+ + 2 O2 first half reaction via intermediate Nomega-hydroxy-L-arginine Bacillus subtilis 2 Nomega-hydroxy-L-arginine + 2 NADP+ + 2 H2O
-
 ?
1.14.13.39 2 L-arginine + 3 NADPH + 3 H+ + 4 O2
-
 Staphylococcus aureus 2 citrulline + 2 nitric oxide + 3 NADP+ + 4 H2O
-
 ?
1.14.13.39 2 L-arginine + 3 NADPH + 3 H+ + 4 O2
-
 Bacillus subtilis 2 citrulline + 2 nitric oxide + 3 NADP+ + 4 H2O
-
 ?
1.14.13.39 2 L-arginine + 3 NADPH + 3 H+ + 4 O2 overall reaction, two-step oxidation of L-arginine using an O2-dependent mechanism, detailed overview Staphylococcus aureus 2 citrulline + 2 nitric oxide + 3 NADP+ + 4 H2O
-
 ?
1.14.13.39 2 L-arginine + 3 NADPH + 3 H+ + 4 O2 overall reaction, two-step oxidation of L-arginine using an O2-dependent mechanism, detailed overview Bacillus subtilis 2 citrulline + 2 nitric oxide + 3 NADP+ + 4 H2O
-
 ?
1.14.13.39 additional information mechanisms of oxygen activation by NOSs, overview Staphylococcus aureus ?
-
 ?
1.14.13.39 additional information mechanisms of oxygen activation by NOSs, overview Bacillus subtilis ?
-
 ?
1.14.13.39 Nomega-hydroxy-L-arginine + NADPH + H+ + O2 second half reaction via intermediate Nomega-hydroxy-L-arginine Staphylococcus aureus citrulline + nitric oxide + NADP+ + H2O
-
 ?
1.14.13.39 Nomega-hydroxy-L-arginine + NADPH + H+ + O2 second half reaction via intermediate Nomega-hydroxy-L-arginine Bacillus subtilis citrulline + nitric oxide + NADP+ + H2O
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.14.13.39 NOS
-
 Staphylococcus aureus
1.14.13.39 NOS
-
 Bacillus subtilis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.14.13.39 25
-
 assay at Staphylococcus aureus
1.14.13.39 25
-
 assay at Bacillus subtilis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.14.13.39 7.6
-
 assay at Staphylococcus aureus
1.14.13.39 7.6
-
 assay at Bacillus subtilis

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.13.39 heme frequencies of electron transfer, overview Staphylococcus aureus
1.14.13.39 heme frequencies of electron transfer, overview Bacillus subtilis
1.14.13.39 NADPH
-
 Staphylococcus aureus
1.14.13.39 NADPH
-
 Bacillus subtilis